Immunofluorescence localization and ultrastructure of Stewart’s wilt disease bacterium Pantoea stewartii in maize leaves and in its flea beetle vector Chaetocnema pulicaria (Coleoptera: Chrysomelidae)

Ammar, El‐Desouky; Correa, Valdir R.; Hogenhout, Saskia A.; Redinbaugh, Margaret G.

doi:10.1016/j.jmau.2014.01.001

Cited by 9 publications

(10 citation statements)

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“…S. enterica was observed in the mid-and hindgut of M. quadrilineatus immediately after acquisition and up to 48 h post-AAP. Similar studies have also reported the occurrence of the enterobacteria P. stewartii and Erwinia tracheiphila in the mid-and hindguts of their vectors, the corn flea beetle and the striped cucumber beetle, Acalymma vittatum, respectively (30,31). It is noteworthy that there was a significant reduction of S. enterica inside the insect during and after acquisition, evidenced by the differences in the frequencies of fluorescent bacteria observed in the guts of M. quadrilineatus dissected after a 12-h AAP or a 24-or 48-h post-AAP.…”

Section: Discussionsupporting

confidence: 66%

Influence of prgH on the Persistence of Ingested Salmonella enterica in the Leafhopper Macrosteles quadrilineatus

Dundore‐Arias

Groves

Barak

2015

Appl Environ Microbiol

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Phytophagous insects can encounter Salmonella enterica on contaminated plant surfaces and transmit externally adhered and internalized bacteria on and among leaves. Excretion of ingested S. enterica by the leafhopper Macrosteles quadrilineatus has been previously reported; however, the sites of persistence of ingested bacteria remain undetermined. Fluorescence microscopy revealed the presence and persistence of S. enterica in various organs of M. quadrilineatus fed an inoculated diet for 12 h and then moved to two consecutive noninoculated diets for a total of 48 h. Ingested S. enterica was predominantly observed in the filter chamber, midgut, and Malpighian tubules of M. quadrilineatus dissected immediately after acquisition and at 24-and 48-h post-acquisition access periods (post-AAPs). Additionally, we examined the potential roles of the Salmonella pathogenicity island 1 (SPI-1) and SPI-2 type III secretion systems (T3SSs) in the persistence and excretion of ingested S. enterica. In competition assays, a prgH mutant lacking a functional SPI-1 T3SS was recovered at significantly lower levels than the WT in insect homogenates at 24 h post-AAP, and complementation with prgH restored S. enterica persistence in M. quadrilineatus. Moreover, expression of prgH inside M. quadrilineatus was observed up to 48 post-AAP. No differences were observed between the WT and an ssaK mutant lacking a functional SPI-2 T3SS in insect homogenates or between the WT and either mutant in insect excretions. This study provides novel insight into the presence and persistence of S. enterica inside M. quadrilineatus and demonstrates that the SPI-1 T3SS influences the persistence of the pathogen in the gut of a potential vector. Salmonella enterica is the leading cause of bacterial foodborne illness in the United States (1). In the last few decades, salmonellosis outbreaks attributed to S. enterica-contaminated fresh produce have increased, and produce is now considered the most likely vehicle of disease transmission (2). Plant contamination with S. enterica is thought to primarily occur in the field before harvest, and the pathogen has the ability to adhere, colonize, and persist in and on plant surfaces, in some cases for extended periods (3, 4). Domestic and wild animals represent one of the main entry routes for S. enterica to produce fields, where they can directly introduce the bacterial pathogens to plants or agricultural water through excretion of contaminated waste (3). Additionally, synanthropic and coprophagic insects can introduce human bacterial pathogens to produce fields by dispersing bacteria associated with their exoskeletons onto plant surfaces (5). These events increase the chances that phytophagous insects could encounter a human enteric pathogen as a result of feeding or wandering on contaminated plant surfaces. The abundance and distribution of phytophagous insects within agricultural fields suggest that their contamination could influence the dispersal of enteric pathogens in the field (6).The aster leafhopper, Macro...

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Section: Discussionsupporting

confidence: 66%

Influence of prgH on the Persistence of Ingested Salmonella enterica in the Leafhopper Macrosteles quadrilineatus

Dundore‐Arias

Groves

Barak

2015

Appl Environ Microbiol

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“…The strange objects which entered into insect bodies could be pathogenic or symbiotic (Ammar et al, 2014). They would establish defense systems when foreign objects penetrated into their bodies (Chapman, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…stewartii associated with digestive tracts of insect vectors (foregut, midgut, hindgut, and malpigian tubule). Pathogen could be observed in midgut and hindgut until 12 days after acquisition (Ammar et al, 2014). Pathogen would be brought by blood circulation and dissolved in saliva.…”

Section: Resultsmentioning

confidence: 99%

Confirmation on Status of Chaetocnema basalis (Coleoptera: Chrysomellidae) as A Vector of Stewart Wilt Disease

Widodo¹,

Wjonarko

Witjaksono

et al. 2018

JPTI

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Chaetocnema pulicaria and C. denticulata are recognized as vectors of Stewart wilt disease caused by Pantoea stewartii on maize. These insects have not been reported yet in Indonesia, but Stewart wilt disease has been reported in Java and Sumatera Islands. Genus Chaetocnema which presented in Indonesia is C.basalis. It is not cleared whether C. basalis is a vector for Stewart wilt disease like C. pulicaria and C. denticulata. This reseach was aimed to conduct the confirmation on status whether C. basalis have a role as vector of Stewart wilt disease on maize or not. C. basalis imago were collected from maize growing areas in Yogyakarta, and then starved for 24 h. Treatments were applied by placing imago of C. basalis on infected-P. stewartii plants for 72 h. Five insects were then transferred to each plot of healthy plant (1 plot consisted of 5 plants) for 72 h. For control, imago of C. basalis were put on healthy plants for 72 h and five insects were then transferred to other healthy plant (1 plot consisted of 5 plants) for 72 h. Each treatment was repeated three times. On the fifteenth days after transmission, PCR assays were carried out on leaf samples and isolates of bacteria. All sampled leaves analysis showed that there were no Stewart wilt diseases transmission based on PCR assay and bacterial isolates. This concluded that C. basalis is not a vector for Stewart wilt disease on maize.

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“…Several research results revealed that P. stewartii subsp. stewartii is transmitted by insect vectors that feed on diseased plants, which then move to feed on healthy plants (Ammar et al, 2014). Several insects are suspected of being disease vectors; C. pulicaria, Agrotis manchus, C. denticulata, Diabrotica nigricornis, D. undecimpuctata, D. versifera, Hylemya cilicrura (Ministry of Agriculture, 2020).…”

Section: Distribution Of P Stewartii Subsp Stewartii To Host Plantsmentioning

confidence: 99%

Status and Recent Developments of Pantoea stewartii subsp. stewartii Causes the Wilt Disease in Maize in Indonesia: A Review

Najamuddin

Djaya²,

Djaenuddin³

et al. 2022

Caraka Tani J. Sustain. Agric.

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Pantoea stewartii subsp. stewartii is a pathogen that causes Stewart wilt on maize. This disease is important in maize. Infection early in growth can cause yield losses of up to 100%. This review examines the history, recent developments, economic impacts and developments of detection technology of Stewart wilt diseases. This paper was created by reviewing several articles relevant to the purpose of the topic. The article's results revealed that the pathogen underwent many changes, including its name, taxonomy, physiological abilities, status as an important pathogen in many countries and the development of detection technology. Currently, P. stewartii subsp. stewartii belongs to the Erwiniaceae family and has physiological abilities that can be distinguished from bacteria of the same genus and species. This pathogen has been reported to spread to more than 82 countries, including Indonesia, with 18 host plants. Some areas have reported pathogens in Indonesia, but no vector has been written. The biggest economic threat caused by this disease is the industrial production of corn seeds which require strict phytosanitary requirements and are free from pathogens. To anticipate its spread, there are four methods of detection of pathogenic bacteria that are commonly used, but serological and molecular detection technologies are the main recommendations.

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Immunofluorescence localization and ultrastructure of Stewart’s wilt disease bacterium Pantoea stewartii in maize leaves and in its flea beetle vector Chaetocnema pulicaria (Coleoptera: Chrysomelidae)

Cited by 9 publications

References 11 publications

Influence of prgH on the Persistence of Ingested Salmonella enterica in the Leafhopper Macrosteles quadrilineatus

Influence of prgH on the Persistence of Ingested Salmonella enterica in the Leafhopper Macrosteles quadrilineatus

Confirmation on Status of Chaetocnema basalis (Coleoptera: Chrysomellidae) as A Vector of Stewart Wilt Disease

Status and Recent Developments of Pantoea stewartii subsp. stewartii Causes the Wilt Disease in Maize in Indonesia: A Review

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