2006
DOI: 10.1038/nprot.2006.265
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Immunofluorescence in brain sections: simultaneous detection of presynaptic and postsynaptic proteins in identified neurons

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Cited by 128 publications
(137 citation statements)
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“…Double and triple immunofluorescence was performed on cryostat sections as described in ref. 48. See SI Materials and Methods for antibody specification and characterization.…”
Section: Methodsmentioning
confidence: 99%
“…Double and triple immunofluorescence was performed on cryostat sections as described in ref. 48. See SI Materials and Methods for antibody specification and characterization.…”
Section: Methodsmentioning
confidence: 99%
“…Immunohistochemistry of frozen coronal sections (20 μm) of the adult mouse brain was performed as described by Schneider Gasser et al (33) by using the primary antibodies against ephrinA5 (Abcam), EphA4, EphA5 (for both, Abcam or Santa Cruz), pErk1/2 (Santa Cruz), GAD67, TH (Millipore), gephyrin (Synaptic Systems), and GFAP (Sigma Aldrich). The secondary antibodies used were Alexa488-or Alexa594-conjugated goat IgG (Molecular Probes), and specific immunoreactivity was confirmed by performing immunohistochemical analysis without addition of the primary antibody.…”
Section: Methodsmentioning
confidence: 99%
“…Brains were postfixed overnight, dehydrated in 20% sucrose in 0.1 M PBS overnight, cut in 30 m sections, and stored in cryoprotective solution. For analysis of gephyrin distribution (Schneider Gasser et al, 2006), 10 rats (LPS, n ϭ 6; vehicle, n ϭ 4) were anesthetized and decapitated. Brains were dissected and placed in ice-cold aCSF, cut in transverse 300-m-thick sections, placed in gassed aCSF for 20 min and in PFA for 10 min, rinsed in PBS, dehydrated in 30% sucrose in 0.1 M PBS, cut in 14 m sections, and stored at Ϫ20°C for at least 1 h.…”
Section: Methodsmentioning
confidence: 99%