Immunofluorescence assay for diagnosis of strongyloidiasis in immunocompromised patients

Gottardi, Maiara; Paula, Fabiana Martins de; Corral, Marcelo Andreetta; Meisel, Dirce Mary Correia Lima; Costa, Sílvia Figueiredo; Abdala, Edson; Pierrotti, Lı́gia Camera; Yamashiro, Juliana; Chieffi, Pedro Paulo; Gryschek, Ronaldo Cesar Borges

doi:10.3109/23744235.2015.1028096

Cited by 8 publications

(8 citation statements)

References 25 publications

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“…The expression of miR-370-3p significantly increased (Fig. 3B ); therefore, we speculated that miR-370-3p was related to disc degeneration 26 . Furthermore, the results of RT-qPCR indicated that miR-370-3p expression increased in degenerated tissues (Fig.…”

Section: Resultsmentioning

confidence: 91%

Inhibition of intervertebral disc disease progression via the circPKNOX1–miR-370-3p–KIAA0355 axis

et al. 2021

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The molecular mechanism underlying the development of intervertebral disc disease (IVDD) is not completely understood. Circular RNAs (circRNAs) play a significant role in the occurrence and development of various diseases, and studies have shown that circPKNOX1 is involved in the compensatory response of extracellular matrix synthesis and secretion of the nucleus pulposus (NP) cells. However, the mechanism through which circRNAs regulate IVDD progression remains unclear; therefore, in this study, we explored the significance of circPKNOX1 in IVDD. The expression of circRNAs in NP cells of normal and degenerative patients was detected using microarray analysis, and the role of circPKNOX1 in IVDD was confirmed using RT-qPCR. The interaction networks of circRNAs, miRNAs, and miRNA target genes were detected using bioinformatics analysis, RNA fluorescence in situ hybridization, and immunofluorescence analysis. We found that the expression of circPKNOX1 decreased in IVDD cells. The expression of circPKNOX1 in NP cells, observed using RT-qPCR and western blotting, was consistent with that observed using array screening. Overexpression of circPKNOX1 increased the expression of collagen II, aggrecan, and SOX9 and decreased that of ADAMTS4, ADAMTS-5, MMP3, and MMP13. We further demonstrated that circPKNOX1 played the role of a sponge by competitively binding miR-370-3p to reverse the inhibition of KIAA0355 expression. Our findings indicated that circPKNOX1 affected the progression of IVDD by regulating the expression of KIAA0355 via miR-370-3p. Therefore, circPKNOX1-based therapy may serve as an effective IVDD treatment strategy.

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Section: Resultsmentioning

confidence: 91%

Inhibition of intervertebral disc disease progression via the circPKNOX1–miR-370-3p–KIAA0355 axis

et al. 2021

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“…25 In this study, we used two serological techniques, ELISA and IFA, using S. venezuelensis iL3 antigen. These were standardized in previous studies carried out by our group 10,13 with good performance in terms of sensitivity and specificity. As a result, it was possible to observe that the serological tests showed greater positivity than the parasitological methods, reinforcing their high sensitivity.…”

Section: Discussionmentioning

confidence: 99%

“…For the parasitological diagnosis, the analysis of a single fecal sample was analyzed by spontaneous sedimentation, Baermann modified, and agar plate culture techniques. 9 For serological techniques were used heterologous antigens from S. Immunofluorescence assay (IFA) was performed according to Gottardi et al 10 (sensitivity of 95.4% and specificity of 95.8%). Briefly, sections of S. venezuelensis iL3 in the Tissue-Tek OCT compound (Sakura Finetek) were obtained using cryo-microtome.…”

Section: Diagnostic Techniquesmentioning

confidence: 99%

Strongyloides infection screening in transplant candidates: What is the best strategy?

Gryschek,

Corral,

Sitta

et al. 2023

Transplant Infectious Dis

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BackgroundThe potential that Strongyloides stercoralis infection has to cause major morbidity and high mortality when the disseminated form occurs in transplant patients is of particular concern.MethodsIn this study, the objective was to observe S. stercoralis infection in patients who are candidates for transplantation by using parasitological, serological, and molecular techniques and to propose an algorithm for the detection of that infection in transplant candidates.ResultsBy parasitological techniques, 10% of fecal samples were positive. Anti‐Strongyloides antibodies immunoglobulin G were detected in 19.3% and 20.7% of patients by immunofluorescence assay and enzyme‐linked immunosorbent assay, respectively. S. stercoralis DNA was observed in 17.3% of samples by conventional polymerase chain reaction and 32.7% of samples by quantitative polymerase chain reaction (qPCR).ConclusionThe set of results allows us to reinforce that a positive result by parasitological techniques and/or qPCR indicates that the specific treatment should be applied. However, the improvement of diagnostic techniques may suggest changes in the screening for strongyloidiasis in these patients. image

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“…Increased access to newer diagnostics, such as the real‐time polymerase chain reaction, luciferase immunoprecipitation systems, and indirect immunofluorescence assay will be a welcome development . New data show improved sensitivity (95.4%) of the indirect immunofluorescence assay in immunosuppressed patients . However, until these diagnostics become more widely available, a high index of suspicion is required, and preemptive therapy in certain high‐risk patients should be considered.…”

Section: Discussionmentioning

confidence: 99%

Strongyloides hyperinfection following hematopoietic stem cell transplant in a patient with HTLV‐1‐associated T‐cell leukemia

Alpern

Arbefeville

Vercellotti

et al. 2017

Transplant Infectious Dis

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Strongyloides stercoralis has the potential to cause accelerated autoinfection in immunocompromised hosts. Screening tests for strongyloidiasis may be falsely negative in the setting of immunosuppression. We report a case of Strongyloides hyperinfection syndrome in a patient with human T-lymphotropic virus type 1-associated T-cell leukemia early after hematopoietic stem cell transplant. The diagnosis was made by stool ova and parasite examination, despite a negative screening enzyme-linked immunosorbent assay. Because of anticipated prolonged neutropenia, an extended course of treatment was utilized.

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Immunofluorescence assay for diagnosis of strongyloidiasis in immunocompromised patients

Abstract: IFA can be used as a screening method for the detection of S. stercoralis in immunocompromised patients.

Cited by 8 publications

References 25 publications

Inhibition of intervertebral disc disease progression via the circPKNOX1–miR-370-3p–KIAA0355 axis

Inhibition of intervertebral disc disease progression via the circPKNOX1–miR-370-3p–KIAA0355 axis

Strongyloides infection screening in transplant candidates: What is the best strategy?

Strongyloides hyperinfection following hematopoietic stem cell transplant in a patient with HTLV‐1‐associated T‐cell leukemia

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