Immunoexpression of Ki67, p53 and cyclin D1 in osteosarcomas

Abstract

The main malignant tumor of the bone tissue is represented by osteosarcoma, neoplasia with a reserved prognosis and an unpredictable evolution, often aggressive. Cell cycle disruption is one of the complex biomolecular mechanisms involved in the progression of osteosarcomas. In this study, we analyzed the immunoexpression of Ki67, p53 and cyclin D1 for 18 primitive osteosarcomas in relation to the clinicopathological prognosis parameters of the lesions. The results indicated the predominance of lesions in male young patients, with femoral location, most tumors being represented by the osteoblastic type, with high grade, size <8 cm and in advanced stages. Reactions were present in all cases, the high immunoexpression being associated with osteoblastic/epithelioid types (Ki67, cyclin D1, p53), high grade (Ki67, cyclin D1) and advanced stage (Ki67, cyclin D1). The study revealed a positive linear relation of the investigated immunomarkers expression, which indicates their usefulness in identifying lesions with aggressive progression potential.

DJ-1 promotes osteosarcoma progression through activating CDK4/RB/E2F1 signaling pathway

“…It was reported that high expression of DJ-1 was involved in the activation of PI3K-Akt signaling pathway ( 15 ), but AKT inhibitor did not significantly inhibit OS cell proliferation ( Supplementary Figure 2 ). Cell cycle disruption is one of the biomolecular mechanisms of OS ( 17 ). Cell cycle analysis by flow cytometry indicated that DJ-1 overexpression significantly increased the ratio of G2-phase cells, which could accelerate cell cycle and promote cell proliferation.…”

“…Therefore, special attention should be paid to reveal the molecular mechanism of the occurrence and progression of OS for the sake of improving the individualized therapeutic effectiveness. DJ-1 is a novel oncogene with an important transforming activity, and plays vital roles in tumor occurrence, progression, metastasis, oxidative stress and apoptosis ( 17 , 21 ). However, its role in OS and potential molecular mechanism have been rarely reported and poorly understood.…”

Clinicopathological assessment of cancer/testis antigens NY‑ESO‑1 and MAGE‑A4 in osteosarcoma

“…25,26 In the current study, p53 expression was positively correlated with that of NY-ESO-1 and MAGE-A4, suggesting their potential as prognostic biomarkers for OS. Ki-67 is also a well-known marker for assessing cell proliferation and invasion, [27][28][29] and has been reported as a marker for diagnosis of malignancy, proliferation, response to chemotherapy, and prognosis in OS. 29,30 In the current study, Ki-67 expression was a positively correlated NY-ESO and MAGE-A4 suggesting their potential as markers for the diagnosis of malignancy, proliferation, response to chemotherapy, and prognosis for OS.…”

“…Ki-67 is also a well-known marker for assessing cell proliferation and invasion, [27][28][29] and has been reported as a marker for diagnosis of malignancy, proliferation, response to chemotherapy, and prognosis in OS. 29,30 In the current study, Ki-67 expression was a positively correlated NY-ESO and MAGE-A4 suggesting their potential as markers for the diagnosis of malignancy, proliferation, response to chemotherapy, and prognosis for OS. Coexistence of p53 and Ki-67 represents high-grade OS and poor prognosis.…”