Immunoexpression of Heat Shock Protein 70, Glypican 3, Glutamine Synthetase, and Beta-Catenin in Hepatocellular Carcinoma After Liver Transplantation: Association Between Positive Glypican 3 and Beta-Catenin With the Presence of Larger Nodules

Ataíde, Elaine Cristina de; Perales, Simone Reges; Silva, M. G.; Filho, Francisco Júlio Barbosa Lima; Sparapani, A.; Filho, P. F. Latuf; Stucchi, Raquel Silveira Bello; Vassallo, José; Escanhoela, C.A.F.; Boin, I.F.S.F.

doi:10.1016/j.transproceed.2017.01.048

Cited by 10 publications

(6 citation statements)

References 17 publications

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“… 38 This protein is not or only weakly expressed in healthy livers. 39 Our observations reveal that GPC3 is expressed in the pericentral zone of normal (rat) livers. In agreement, Wnt signaling is exclusively active in pericentral hepatocytes.…”

Section: Discussionmentioning

confidence: 58%

Therapeutic efficacy of a Dioscorea membranacea extract in a rat model of hepatocellular carcinoma: Histopathological aspects

Kerdput

Nilbu-Nga

Kaewnoonual

et al. 2021

Journal of Traditional and Complementary Medicine

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Hepatocellular carcinoma (HCC) is most common in adults and has a high mortality rate because of a lack of effective treatment options. We investigated the effect of a medicinal plant as a potential source of drugs against HCC. The rhizomes of Dioscorea membranacea Pierre (DM), Hua-Khao-Yen in Thai, are commonly used as ingredients for alternative treatment of cancer in Thailand. In this study, the anticancer effects of DM extract in HCC-bearing rats were evaluated with respect to gross morphology, histopathology, and leakage of liver enzymes. In untreated HCCs, typical features of liver cancer, including hepatic nodules, thick-cell cords, and pseudoglandular cell arrangements, were observed. In addition, the HCCs showed abnormal reticulin patterns and a high glypican3 expression. In HCC-bearing rats treated with DM the cancer areas and reticulin expression were significantly reduced compared to the untreated group ( p < 0.01). Sorafenib, the standard drug to treat HCC, reduced the cancer area further, but increased leakage of liver enzymes and decreased serum albumin concentration, indicating liver toxicity. These findings suggest that DM has an anticancer effect on HCCs in an animal model in vivo with potentially less severe side effects than sorafenib. Therefore, further studies of DM’s mechanism of action in HCC should be carried out.

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Section: Discussionmentioning

confidence: 58%

Therapeutic efficacy of a Dioscorea membranacea extract in a rat model of hepatocellular carcinoma: Histopathological aspects

Kerdput

Nilbu-Nga

Kaewnoonual

et al. 2021

Journal of Traditional and Complementary Medicine

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“…8 Transcatheter arterial chemoembolization (TACE) is a choice for treating HCC at intermediate stage, which improves survival in patients with HCC. 9,10 However, it is suggested that the recurrence range of HCC is from 6% to 26% and the post-surgical recurrence event of HCC was still frequent, 11,12 which made it urgent to seek for a candidate therapeutic approach for the patients with HCC.…”

Section: Introductionmentioning

confidence: 99%

Retracted: Effect of the Wnt/β‐catenin signaling pathway on apoptosis, migration, and invasion of transplanted hepatocellular carcinoma cells after transcatheter arterial chemoembolization in rats

Wang

2018

J of Cellular Biochemistry

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This study aims to investigate the influence of the Wnt/β-catenin signaling pathway on apoptosis, migration, and invasion of transplanted hepatocellular carcinoma (HCC) cells after transcatheter arterial chemoembolization (TACE) in rat models. A total of 80 rats were grouped into sham, TACE, Wnt-C59, and TACE + Wnt-C59 groups (n = 20). Ten days after model establishment, 10 rats in each group were executed to perform pathological examination and follow-up experiment, and the remaining 10 rats in each group were reared to observe the survival condition. RT-qPCR and Western blotting were applied to determine the expressions of Wnt1, β-catenin, cyclin D1, c-met, vimentin, E-cadherin, and vascular endothelial growth factor (VEGF). ELISA was performed to measure the serum alpha-fetoprotein (AFP) content of rats. Flow cytometry was used to evaluate cell apoptosis rate and transwell assay to examine cell migration and invasion. Compared with the TACE group, the Wnt-C59 and TACE + Wnt-C59 groups showed increased apoptosis and survival time (the TACE + Wnt-C59 group > the Wnt-C59 group). Compared with the sham group, the TACE + Wnt-C59 groups showed decreased cancer tissue weight and expressions of Wnt1, β-catenin, cyclin D1, vimentin, c-met, and VEGF, but increased E-cadherin expression. Compared with the TACE group, the Wnt-C59 and TACE + Wnt-C59 groups showed decreased AFP level, migration, and invasion (the TACE + Wnt-C59 group < the Wnt-C59 group). These findings indicate inhibition of the Wnt/β-catenin signaling pathway improves therapeutic effect on TACE via suppressing migration, invasion, and promoting apoptosis of transplanted HCC cells in rats.

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“…A study of HCC patients who underwent LT found that beta-catenin expression in the liver was associated with HCC recurrence after LT[ 142 ]. However, in other recently published study, no association was found between beta-catenin expression in the liver prior to LT and the survival of HCC patients[ 143 ]. Therefore, additional research is necessary to establish the prognostic role of beta-catenin expression in the liver in HCC patients prior to LT.…”

Section: Biomarkersmentioning

confidence: 95%

New prognostic biomarkers of mortality in patients undergoing liver transplantation for hepatocellular carcinoma

Lorente

2018

WJG

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The outcome prediction of hepatocellular carcinoma (HCC) patients undergoing liver transplantation (LT) was classically established using various macromorphological factors and serum alpha-fetoprotein levels prior to LT. However, other biomarkers have recently been reported to be associated with the prognosis of HCC patients undergoing to LT. This review summarizes clinical data on these new biomarkers. High blood levels of malondialdehyde, total antioxidant capacity, caspase-cleaved cytokeratin-18, soluble CD40 ligand, substance P, C-reactive protein, and vascular endothelial growth factor, increased neutrophil to lymphocyte ratio and platelet to lymphocyte ratio in blood, high peripheral blood expression of human telomerase reverse transcriptase messenger ribonucleic acid, and high HCC expression of dickkopf-1 have recently been associated with decreased survival rates. In addition, high blood levels of des-gamma-carboxy prothrombin, and high HCC expression of glypican-3, E-cadherin and beta-catenin have been associated with increased HCC recurrence. Additional research is necessary to establish the prognostic role of these biomarkers in HCC prior to LT. Furthermore, some of these biomarkers are also interesting because their potential modulation could help to create new research lines for improving the outcomes of those patients.

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Immunoexpression of Heat Shock Protein 70, Glypican 3, Glutamine Synthetase, and Beta-Catenin in Hepatocellular Carcinoma After Liver Transplantation: Association Between Positive Glypican 3 and Beta-Catenin With the Presence of Larger Nodules

Cited by 10 publications

References 17 publications

Therapeutic efficacy of a Dioscorea membranacea extract in a rat model of hepatocellular carcinoma: Histopathological aspects

Therapeutic efficacy of a Dioscorea membranacea extract in a rat model of hepatocellular carcinoma: Histopathological aspects

Retracted: Effect of the Wnt/β‐catenin signaling pathway on apoptosis, migration, and invasion of transplanted hepatocellular carcinoma cells after transcatheter arterial chemoembolization in rats

New prognostic biomarkers of mortality in patients undergoing liver transplantation for hepatocellular carcinoma

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