Immunoexpression of Bmi-1, CK15, Bcl-2 in different types of basal cell carcinomas

Abstract

Introduction: Basal cell carcinoma (BCC) occurs in aggressive and non-aggressive forms. The expression of immunohistochemical markers varies in different types of BCC. Aim: Immunohistochemical analysis of selected proteins in BCCs. Material and methods: The immunohistochemical method was used to examine the immunoexpression of Bmi-1, CK15 and Bcl-2 in 56 cases of BCC divided into four groups. Results: Positive Bmi-1 staining 3-4+ level (nodular type) was seen in 91.3% of samples, 4+ (infiltrative) in 92.3%, 4+ (nodular/infiltrative) -69.2%, 3+ -30.8%, in BSC 3+ -42.8%, and 28.6% each for 2+ and 4+. Low grade positivity (0-1+) in CK15 staining was present in 52.1% of nodular BCC, 46.2% -nodular/infiltrative, 92.3% -infiltrative, and 100% -BSC, but levels 2-3+ in nodular BCC in 47.8%, nodular/infiltrative BCC -53.8%, infiltrative -7.7%. Bcl-2 positivity (3-4+) was revealed in nodular BCC in 95.6%, (1-2+) in 100% of BSC, infiltrative and infiltrative/nodular BCC, but the lowest (0-1+) in 76.9% of nodular/infiltrative BCC, 71.4% of BSC, and in 38.4% of infiltrative BCC. Conclusions: Positive Bmi-1 staining was the highest in the aggressive infiltrative subtype of BCCs, whereas the lowest in basosquamous cell carcinomas (BSC). Infiltrative BCC was characterized by a lower level of CK15 expression than nodular BCC and nodular/infiltrative BCC. Differentiation of Bcl-2 expression depended on the type of tumour; the highest level was found in nodular BCC, low grade in nodular/infiltrative and infiltrative BCCs, and BSC.

Eccrine Poroma with Concurrent Basal Cell Carcinoma: A Rare Combination

“…BCL-2 is diffusely positive in most BCC, but in TE it is mainly positive in the outermost epithelial cells. 20 CD10 is a marker of germinal central cells and their derived lymphomas. CD10 was positive for basal-like cells in BCC and negative for peripheral stromal cells, but the opposite was true in TE, which was positive for peripheral stromal cells and negative for basal-like cells.…”

“…BCL-2 is diffusely positive in most BCC, but in TE it is mainly positive in the outermost epithelial cells. 20 CD10 is a marker of…”

Correlation of ultrasound features with Bcl‐2 protein expression in basal cell carcinomas (BCCs)

“…Immunohistochemistry was performed using the standard streptavidin‐peroxidase method 11 with Bcl‐2 mouse anti‐human monoclonal antibody from Beijing Zhongshan Biotechnology Company at a dilution of 1:50. After surgical removal, specimens were formalin‐fixed, paraffin‐embedded, and sectioned at 4 μm thickness.…”

“…Immunohistochemistry was performed using the standard streptavidinperoxidase method 11 with Bcl-2 mouse anti-human monoclonal antibody from Beijing Zhongshan Biotechnology Company at a dilution of 1:50.…”