Immunoexpression of Angiogenesis, Nitric Oxide Synthase, and Proliferation Markers in Gingival Samples of Patients With Aggressive and Chronic Periodontitis

Abstract

The analysis demonstrates a higher expression of all immunologic markers particularly in subjects with aggressive periodontitis. In summary, the data from this pilot investigation suggests that VEGF is an important factor in the pathogenesis of the aggressive and chronic forms of periodontitis.

Expression of angiogenesis-stimulating factors (VEGF, CD31, CD105) and angiogenetic index in gingivae of patients with chronic periodontitis

“…In our study, we applied monoclonal mouse anti-human antibodies for parallel detection of three VEGF isoforms: VEGF-A (VEGF-121), VEGF-B (VEGF-165) and VEGF-C (VEGF-189) produced by DAKO (clone VG1). Also the applied antibody dilution was 1:50 in the work of Artese et al, while we applied a dilution of 1:100 [21]. Similarly to our results, the authors failed to note epithelial localization of VEGF, as documented and highlighted in the study by Lucerini et al [20].…”

Expression of angiogenesis-stimulating factors (VEGF, CD31, CD105) and angiogenetic index in gingivae of patients with chronic periodontitis

“…In gingival samples obtained from patients with a healthy periodontium, the expression of VEGF was negligible and involved individual flat ECs of gingival blood vessels, in accordance with the results of other investigators [16,21]. However, the obtained results do not allow the confirmation of localization of VEGF mainly in microvessel endothelium or localization manifesting higher intensity than that in blood vessels of control gingivae, as demonstrated by other authors [21]. The study by Artese et al provided no source of the antibodies applied to localize VEGF in the studied tissue material, which may be of significance for localization of a specific isoform of the protein.…”

“…Ratio of entire expression of endoglin to that of PECAM-1 (angiogenetic ratio, AR1) and of VEGF to that of PECAM-1 (angiogenetic ratio, AR2) in all groups of patients (CP, C1 and C2) www.fhc.viamedica.pl sels. In gingival samples obtained from patients with a healthy periodontium, the expression of VEGF was negligible and involved individual flat ECs of gingival blood vessels, in accordance with the results of other investigators [16,21]. However, the obtained results do not allow the confirmation of localization of VEGF mainly in microvessel endothelium or localization manifesting higher intensity than that in blood vessels of control gingivae, as demonstrated by other authors [21].…”

“…The data of Artese et al (2010) suggests that VEGF is an important factor in the pathogenesis of the aggressive and chronic forms of periodontitis, but further investigations with a large population sample are needed to identify how VEGF is involved in the tissue inflammation-associated processes [21]. In pathological tissues, various cellular sources of VEGF have been documented [19,[20][21][22].…”

Upregulation of Gingival Tissue miR-200b in Obese Periodontitis Subjects

“…Angiogenesis plays a pivotal role in periodontitis, as it facilitates transporting oxygen and nutrients to the injury site and removal of the cellular debris from the inflamed tissue (Artese et al 2010). Downregulation of endothelial miR-200b enables upregulation of GATA2 transcription factor activity, which regulates the promoters of many endothelial genes, and thus, successful angiogenic outcome (Chan et al 2012).…”