Immunoenhancing Effects of Bovine Glycomacropeptide and Its Derivatives on the Proliferative Response and Phagocytic Activities of Human Macrophagelike Cells, U937

Li, Esther W. Y.; Mine, Yoshinori

doi:10.1021/jf0355102

Cited by 85 publications

(64 citation statements)

References 14 publications

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“…Thus, in spite of the inability of PES-1 and PES-5 to stimulate Th cells, they may influence immune responses through the activation of monocytes. This finding is in line with previous studies reporting the activation of macrophage function by whey protein hydrolysates (Gattegno, Migliore-Samour, Saffar, & Jolles, 1988;Li & Mine, 2004).…”

Section: Cytokine Profile Depends On B-lg Peptidic Fractionsupporting

confidence: 93%

Immunomodulatory activities of whey β-lactoglobulin tryptic-digested fractions

Rodríguez‐Carrio

Fernández

Riera

2014

International Dairy Journal

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Section: Cytokine Profile Depends On B-lg Peptidic Fractionsupporting

confidence: 93%

Immunomodulatory activities of whey β-lactoglobulin tryptic-digested fractions

Rodríguez‐Carrio

Fernández

Riera

2014

International Dairy Journal

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“…This peptide inhibits lipopolysaccharide and phytohemagglutinin-induced proliferation of mouse splenocytes and rabbit Peyer's patch cells [15], upregulates the expression of IL-1 receptor antagonist-like component in mouse lymphocytes [16] and suppresses the expression of IL-2 receptor on mouse CD4+ T cells [17]. It enhances the proliferation and the phagocytic activities of the human macrophage cell line U937 [18] and upregulates the secretion of tumor necrosis factor (TNF)-α, IL-1β, and IL-18 in human monocyte THP-1 cells [19]. However, there is no information about the effect of GMP on the activation of mast cells, which are one of the main effector cells in allergy.…”

Section: Introductionmentioning

confidence: 99%

Novel Mechanisms Underlying the Therapeutic Effect of Glycomacropeptide on Allergy: Change in Gut Microbiota, Upregulation of TGF-β, and Inhibition of Mast Cells

Jiménez¹,

Cervantes‐García²,

Muñoz³

et al. 2016

Int Arch Allergy Immunol

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Background: The prevalence of allergic diseases is globally increasing. We have previously described that glycomacropeptide (GMP), a bioactive milk peptide, has therapeutic value in experimental models of skin hypersensitivity, anaphylaxis, and asthma, as it prevents an excessive T helper type 2 cell immune response. The aim of this study was to analyze the effect of GMP on key elements directly involved in the development or control of allergy, in order to improve the precise knowledge about its mechanism of action. Methods: Rats were systemically sensitized with ovalbumin and orally treated with GMP. Levels of Lactobacillus, Bifidobacterium, and Bacteroides were analyzed in their feces. Splenocytes were isolated and the production of transforming growth factor (TGF)-β by allergens was measured. Intradermal skin reactions were developed to evaluate in vivo activation of mast cells. Peritoneal mast cells were isolated and activated by the allergen, and histamine secretion was determined. Results: GMP administration increased the amount of intestinal Lactobacillus and Bifidobacterium of allergen-sensitized animals after 3 days of treatment. The increase in Bacteroides was also significant, but only after 17 days of GMP administration. Ten days after treatment cessation, Lactobacillus and Bacteroides were still elevated. GMP intake also elevated the production of TGF-β in the splenocytes of sensitized animals. In addition, treatment with GMP attenuated mast cell activation by the allergen and inhibited histamine secretion, without affecting the number of mast cells. Conclusions: The prebiotic action of GMP on allergy-protective microbiota, an increase in TGF-β production, and a reduction in mast cell response to allergens are novel mechanisms that explain the antiallergic activity of GMP.

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“…In vitro studies indicate that GMP binds cholera and E. coli enterotoxins and inhibits bacterial and viral adhesions (36), inhibits adhesion of cariogenic bacteria with usage in toothpaste (2), and modulates immune response (27,47). In vivo studies indicate that oral administration of GMP shows substantial anti-inflammatory effects in rat models of colitis and ileitis (29,46), increases zinc absorption in rhesus monkeys (22), and may promote satiety in humans (9,26,30,56).…”

mentioning

confidence: 99%

Glycomacropeptide, a low-phenylalanine protein isolated from cheese whey, supports growth and attenuates metabolic stress in the murine model of phenylketonuria

Solverson

Murali²,

Brinkman³

et al. 2012

American Journal of Physiology-Endocrinology and Metabolism

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Solverson P, Murali SG, Brinkman AS, Nelson DW, Clayton MK, Yen CL, Ney DM. Glycomacropeptide, a low-phenylalanine protein isolated from cheese whey, supports growth and attenuates metabolic stress in the murine model of phenylketonuria. Am J Physiol Endocrinol Metab 302: E885-E895, 2012. First published January 31, 2012; doi:10.1152/ajpendo.00647.2011.-Phenylketonuria (PKU) is caused by a mutation in the phenylalanine (phe) hydroxylase gene and requires a low-phe diet plus amino acid (AA) formula to prevent cognitive impairment. Glycomacropeptide (GMP) contains minimal phe and provides a palatable alternative to AA formula. Our objective was to compare growth, body composition, and energy balance in Pah enu2 (PKU) and wild-type mice fed low-phe GMP, low-phe AA, or high-phe casein diets from 3-23 wk of age. The 2 ϫ 2 ϫ 3 design included main effects of genotype, sex, and diet. Fat and lean mass were assessed by dual-energy X-ray absorptiometry, and acute energy balance was assessed by indirect calorimetry. PKU mice showed growth and lean mass similar to wild-type littermates fed the GMP or AA diets; however, they exhibited a 3-15% increase in energy expenditure, as reflected in oxygen consumption, and a 3-30% increase in food intake. The GMP diet significantly reduced energy expenditure, food intake, and plasma phe concentration in PKU mice compared with the casein diet. The high-phe casein diet or the low-phe AA diet induced metabolic stress in PKU mice, as reflected in increased energy expenditure and intake of food and water, increased renal and spleen mass, and elevated plasma cytokine concentrations consistent with systemic inflammation. The low-phe GMP diet significantly attenuated these adverse effects. Moreover, total fat mass, %body fat, and the respiratory exchange ratio (CO 2 produced/O2 consumed) were significantly lower in PKU mice fed GMP compared with AA diets. In summary, GMP provides a physiological source of low-phe dietary protein that promotes growth and attenuates the metabolic stress induced by a high-phe casein or low-phe AA diet in PKU mice. indirect calorimetry; energy balance; splenomegaly; cytokines; amino acid metabolism

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Immunoenhancing Effects of Bovine Glycomacropeptide and Its Derivatives on the Proliferative Response and Phagocytic Activities of Human Macrophagelike Cells, U937

Cited by 85 publications

References 14 publications

Immunomodulatory activities of whey β-lactoglobulin tryptic-digested fractions

Immunomodulatory activities of whey β-lactoglobulin tryptic-digested fractions

Novel Mechanisms Underlying the Therapeutic Effect of Glycomacropeptide on Allergy: Change in Gut Microbiota, Upregulation of TGF-β, and Inhibition of Mast Cells

Glycomacropeptide, a low-phenylalanine protein isolated from cheese whey, supports growth and attenuates metabolic stress in the murine model of phenylketonuria

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