Electron Microscopy of Plant Pathogens 1991
DOI: 10.1007/978-3-642-75818-8_7
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Immunoelectron Microscopy for Virus Identification

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Cited by 16 publications
(10 citation statements)
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“…IEM was performed as described with a few modifications [1718]. Briefly, cells were fixed with 4% paraformaldehyde and 1% glutaral in 0.1 M phosphate buffer at 4°C for 4 hrs.…”
Section: Methodsmentioning
confidence: 99%
“…IEM was performed as described with a few modifications [1718]. Briefly, cells were fixed with 4% paraformaldehyde and 1% glutaral in 0.1 M phosphate buffer at 4°C for 4 hrs.…”
Section: Methodsmentioning
confidence: 99%
“…Virus identification was carried out by using the immunoelectron-microscopical method of decoration with antisera (Milne and Luisoni, 1977 For serological investigations standard DAS-ELISA as described by Clark and Adams (1977) and the plate trapped antigen (PTA)-ELISA according to Richter et al (1994) were carried out. Sample preparation and SDS-PAGF for "Western blotting were used as described by Laemmli (1970).…”
Section: Methodsmentioning
confidence: 99%
“…To improve the attachment of virus particles, grids can be coated by an antiserum prior to incubation with the leaf extract. This ‘trapping’ of particles results in a selective immune‐enrichment of virus particles on the grid (Milne, ). Another advantage of the layer of antibodies and other serum constituents is the inhibition of non‐specific binding of structures from the sample preparation.…”
Section: General Instructionsmentioning
confidence: 99%
“…Following incubation and washing, the grid can either be negatively stained and observed or a secondary antibody (which may be conjugated to gold particles) can be used prior to negative staining. In this case, the sample is incubated with a drop of diluted anti‐virus antiserum, so that antibodies can attach to the virus particles (Milne, ). Decoration with homologous antibodies (IEM) followed by staining will usually allow virus identification, provided that the analytical specificity of the antibodies is known.…”
Section: General Instructionsmentioning
confidence: 99%