Immunoelectron-microscopical localization of in vivo-bound complement C3 in bullous pemphigoid with the use of the peroxidase-antiperoxidase multistep technique

Maciejewski, Wojciech; Braun‐Falco, Otto; Scherer, R.

doi:10.1007/bf00694630

Cited by 9 publications

(2 citation statements)

References 19 publications

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“…In thc present study, the immunoperoxidase method was used to mvestigate ultrastruc tural localization of the binding sites of anti-BMZ antibodies (BMZ-Ab) in vitro, according to the procedures described in the previous reports (3)(4)(5)(6)(7)(8)(9)(10)(11), with slight modifications, paying attention to the binding sites of both BMZ-Ab demonstrable by the indirect immunoperoxidase (IIP) method (IlP demonstrable BMZ-Ab) and complement lixing BMZ-Ab demonstrated with the complement immunoperoxidase (CIP) method (CF BMZ Ab) comparatively.…”

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confidence: 99%

Ultrastructural localization of in vitro binding sites of circulating anti-basement membrane zone antibodies in bullous pemphigoid

Yamasaki,

Nishikawa

1983

Acta Derm Venereol

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Ultrastructural localization of the in vitro binding sites of circulating anti-basement membrane zone antibodies was investigated using two immunoperoxidase methods: the ordinary IgG indirect immunoperoxidase method and the complement immunoperoxidase method. The results indicate that the binding sites of the circulating autoantibodies were diverse. It is postulated that those antibodies demonstrable by the ordinary IgG indirect method and those antibodies possessing capacity to fix complement should bind to different sites of the basement membrane zone. Conventional electron microscopic observation of one lesional skin from bullous pemphigoid confirmed this possibility. (Received December 23, 1982.)

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confidence: 99%

Ultrastructural localization of in vitro binding sites of circulating anti-basement membrane zone antibodies in bullous pemphigoid

Yamasaki,

Nishikawa

1983

Acta Derm Venereol

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“…However, ultrastructural-localization of bound C3 in pemphigus skin has not been examined in any of these reports. A number of IEM observations of bound immunoglobulins and C3 have been reported in bullous dermatoses other than pemphigus and in lupus erythematosus (10)(11)(12)(13)(14)(15)(16).…”

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confidence: 99%

Immunopathological Study on Perilesional Pemphigus Skin

Yamasaki

1981

The Journal of Dermatology

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Seven perilesional skin samples which appeared to be normal were obtained from patients with pemphigus and studied by means of direct immunofluorescence and horseradish peroxidase labeled antibody methods. Light microscopic observations revealed that in vivo bound IgG and C3 were localized in the intercellular cement substance region of the epidermis in both the direct immunofluorescence and horseradish peroxidase labeled antibody method in exactly the same fashion. Immunoelectronmicroscopic observations revealed that bound IgG and C3 were localized exclusively in the epidermal intercellular cement substance region, but not in any intracellular organellae, pinocytotic vesicles, cytosol, basal lamina or upper dermis. Bound IgG was detected in all seven cases. Bound C3 was detected in four of the seven cases. Both of these globulins were localized exclusively in the nondesmosomal and desmosomal epidermal intercellular cement substance region, apparently in the same fashion. It was suggested immunoelectronmicroscopically that not only IgG, but also the complement system might be involved in the formation of the skin lesion in pemphigus.

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Acquired Epidermolysis Bullosa With the Clinical Feature of Brunsting-Perry Cicatricial Bullous Pemphigoid

Kurzhals

Stolz²,

Meurer³

et al. 1991

Arch Dermatol

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A 56-year-old woman with the typical clinical feature of cicatricial bullous pemphigoid of the Brunsting-Perry type was studied. Histologic examination of a lesion skin biopsy specimen demonstrated a subepidermal blister. Direct immunofluorescence microscopy revealed linear deposits of IgG, IgM, and C3 located on both the roof and the floor of the blister. Immunofluorescence antigen mapping using cryostat sections of a spontaneous blister and antisera against defined basement membrane components localized the bullous pemphigoid antigen and type IV collagen in the roof of the blister. This dermal type of blister formation was confirmed by electron microscopy, which showed the cleavage level below the lamina densa. In direct immunoelectron microscopy, granular deposits of C3 and IgG were found attached to and just beneath the lamina densa in a pattern identical to the distribution of anchoring fibrils. These findings are diagnostic of acquired epidermolysis bullosa, a blistering disease that has much more clinical heterogeneity than previously suggested.

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Immunoelectron-microscopical localization of in vivo-bound complement C3 in bullous pemphigoid with the use of the peroxidase-antiperoxidase multistep technique

Cited by 9 publications

References 19 publications

Ultrastructural localization of in vitro binding sites of circulating anti-basement membrane zone antibodies in bullous pemphigoid

Ultrastructural localization of in vitro binding sites of circulating anti-basement membrane zone antibodies in bullous pemphigoid

Immunopathological Study on Perilesional Pemphigus Skin

Acquired Epidermolysis Bullosa With the Clinical Feature of Brunsting-Perry Cicatricial Bullous Pemphigoid

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