Immunoelectron microscopic study of the GH cell in the anterior pituitary gland of normal human fetus

Tachibana, Toshiaki; Itō, Takayasu; Kwon, Oh‐Chol

doi:10.1002/ar.1092390208

Cited by 4 publications

(4 citation statements)

References 32 publications

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“…These cells were observed at week 9; for Asa et al (1986) these cells were also the first detected, at week 8, but at the same time as adrenocorticotrophin and β endorphin. Other authors demonstrated an early presence of GH cells in an electron microscopy study (Tachibana et al 1994) and reported that one ultrastructural type of GH cells was present in the first half of gestation and a second type in the second half. GH is one of the regulatory factors of fetal growth (Waters & Kaye, 2002), its receptors being widespread (Hill et al 1992;Werther et al 1993) and expressed at week 8 (Simard et al 1996).…”

Section: Discussionmentioning

confidence: 92%

“…(1986) these cells were also the first detected, at week 8, but at the same time as adrenocorticotrophin and β endorphin. Other authors demonstrated an early presence of GH cells in an electron microscopy study (Tachibana et al. 1994) and reported that one ultrastructural type of GH cells was present in the first half of gestation and a second type in the second half.…”

Section: Discussionmentioning

confidence: 95%

“…Immunohistochemical studies on the cytogenesis, development and differentiation of the pituitary gland have also been carried out in several vertebrate groups (Chatelain et al 1979;Batista et al 1989;Japon et al 1994;Allaerts et al 1999), although in humans these studies are scarce (Baker & Jaffe, 1975;Asa et al 1986;Tachibana et al 1994), with apparently no studies of peptides in the developmental period.…”

Section: Introductionmentioning

confidence: 99%

“…1999), although in humans these studies are scarce (Baker & Jaffe, 1975; Asa et al. 1986; Tachibana et al. 1994), with apparently no studies of peptides in the developmental period.…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical distribution of regulatory peptides in the human fetal adenohypophysis

et al. 2008

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We have studied here the cellular distribution of several regulatory peptides in hormone-producing cells of the human pituitary during the fetal period. Immunohistochemistry was used to show the expression of several regulatory peptides, namely Angiotensin-II, Neurotensin and Galanin, at successive gestational stages and their colocalization with hormones in the human fetal adenohypophysis. Somatotrophs, gonadotrophs and thyrotrophs were differentiated earliest. At gestational week 9, Angiotensin-II immunoreactivity was co-localized only with growth hormone immunoreactivity in somatotrophs, one of the first hormone-producing cells to differentiate. This co-localization remained until week 37. Neurotensin immunoreactivity was present in gonadotrophs and thyrotrophs in week 23, after FSH and TSH hormone differentiation. Galanin immunoreactivity was present in all hormone-producing cell types except corticotrophs. The different pro-opiomelanocortin-derived peptides were detected at different stages of gestation and adrenocorticotrophic hormone immunoreaction was the last to be detected. Our results show an interesting relationship between regulatory peptides and hormones during human fetal development, which could imply that these peptides play a regulatory role in the development of pituitary function.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

“…1999), although in humans these studies are scarce (Baker & Jaffe, 1975; Asa et al. 1986; Tachibana et al. 1994), with apparently no studies of peptides in the developmental period.…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical distribution of regulatory peptides in the human fetal adenohypophysis

et al. 2008

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`Gangliocytomas' of the Pituitary

Geddes

Jansen

Robinson

et al. 2000

The American Journal of Surgical Pathology

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Hamartomatous or neoplastic ganglion cells in the sella turcica are an unusual cause of symptoms. They have been reported in association with a functioning or nonfunctioning pituitary adenoma, with pituitary cell hyperplasia, and occasionally as masses unassociated with an adenoma, again with variable endocrinologic findings. Fewer than 50 cases of intrasellar ganglion cell lesions have been reported in the literature, only six of them associated with Cushing's syndrome. We describe the clinicopathologic features of another eight patients, three of whom presented with acromegaly, four with apparently nonfunctioning adenohypophyseal masses, and one with Cushing's syndrome. On histology, six of them were found to have sparsely granulated growth hormone (GH)-producing adenomas with ganglion cell areas, one appeared to have a gangliocytoma not associated with an adenoma, whereas the eighth had a ganglion cell lesion in the posterior pituitary. The morphologic and immunohistochemical findings suggest that the ganglion cell component of seven of these tumors has resulted from neuronal differentiation in a GH-producing adenoma, despite the lack of demonstrable adenoma in one case. A true sellar "gangliocytoma" or hamartoma of ectopic hypothalamic-type neurons appears to be a rarer explanation for the presence of ganglion cells in a pituitary biopsy.

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Immunocytochemical Study of the GH Cells in the Anterior Pituitary Gland of Human Fetus II. Anencephalic Fetus

Tachibana

Itō

2003

Human Cell

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In order to elucidate the effects of hypothalamic regulation on the morphology of GH cells, light and electron microscopic immunocytochemical examinations were carried out comparing GH cells in the anterior pituitary gland of anencephalic fetus with those of normal fetuses. Three types of GH cells were identified in the anterior pituitary gland of anencephalic fetus as well as in the normal fetus. Type-I is a small, round cell containing a few small secretory granules. Type-III is a large, polygonal cell with numerous large secretory granules. Type-II is a polygonal cell with medium-sized secretory granules. The Type-II GH cell was predominant in both anencephalic and normal fetuses. The most striking difference between anencephalic and normal fetuses was the presence of atypical forms of the Type II cell. These were polygonal cells containing secretory granules, which were either immunopositive or immunonegative to anti-human GH (anti-hGH) serum. Furthermore, two other types of GH cells were identified. The somatomammotroph (SM cell) contained GH and PRL in different granules within the same cell. Also, a different type of the GH cell was noted containing two varieties of secretory granules; one was immunolabeled only with anti-hGH and the other was not immunolabeled to either anti-hGH or anti-human PRL (anti-hPRL). From these results, we suggest that an absence of hypothalamic regulation in the anencehpalic does not seriously modify GH cell morphology but induces an altered GH storage pattern in some of the cells.

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Immunoelectron microscopic study of the GH cell in the anterior pituitary gland of normal human fetus

Cited by 4 publications

References 32 publications

Immunohistochemical distribution of regulatory peptides in the human fetal adenohypophysis

Immunohistochemical distribution of regulatory peptides in the human fetal adenohypophysis

`Gangliocytomas' of the Pituitary

Immunocytochemical Study of the GH Cells in the Anterior Pituitary Gland of Human Fetus II. Anencephalic Fetus

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