Immunoelectron microscopic localization of human T-cell leukemia virus type-I antigens.

Immunoelectron microscopic study of HTLV-II-producing cells with an anti-envelope gp46 monoclonal antibody

“…Ultrastructurally, the HTLV-I core protein was mainly detected on both the virions themselves and the cell surface [1,13,15], and the pX product was observed in the intracytoplasmic region [8]. In this study, we demonstrated HTLV-II gp46 expression not only on the cell surface and virus particles but in the cytoplasm as well.…”

Detection of adult T-cell leukemia-associated antigens by direct immunoperoxidase microscopy with Fab'-peroxidase conjugates prepared with a maleimide compound.

“…Molt-4 cells were not stained in the control experiments. In our previous study, immunoperoxidase staining with the antisera to p24 and with monoclonal antibody to HTLV p l9 and p28 was not observed on the inner and outer leaflets of the nuclear membrane (14). In this study, the immunoperoxidase staining was demonstrated for the first time on the both inner and outer leaflets of the nuclear membrane using the direct immunoelectron microscopy with sera from ATL healthy carriers.…”

“…Electron microscopy with the immunoferritin method revealed that the ferritin-labeled antibodies could not penetrate the cellular and viral membranes (2,13,15,16). Indirect immunoperoxidase staining has been employed for the observation of the detailed distribution of ATLA in MT-2 cells and HTLV-I (14).…”

“…In our previous study (14), the location of ATLA in HTLV-I-producing MT-2 cells was observed by light and electron microscopy with the indirect immunoperoxidase method using sera of ATL patients, the monoclonal antibody to HTLV p19 and p28, and antisera to p24. ATLA was demonstrated on some areas of the cisternal membrane of the endoplasmic reticulum and the nuclear membrane, but not in the nucleus.…”

“…ATLA in HTLV-I-producing MT-2 cells have been detected by electron microscopy using the immunoferri.tin method (2,13,15,16). Indirect immunoperoxidase staining of ATLA with sera of ATL patients, the monoclonal antibody to ATLV p19 and p28 (14,23), and antisera to p24 in frozen-sectioned MT-2 cells were observed in our previous study (14).…”