Immunodominant antigen of Actinobacillus actinomycetemcomitans Y4 in high-responder patients

Califano, Joseph V.; Schenkein, Harvey A.; Tew, John G.

doi:10.1128/iai.57.5.1582-1589.1989

Cited by 82 publications

(69 citation statements)

References 24 publications

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“…The specific strain of bacteria used may account for other differences in the antibody response in these "refractory" patients when compared to published data on antibody responses in other patient populations. For example, serotype antigens on A. antinomycetemcomitans are very immunogenic in humans (Califano et al 1989, Taichman et al 1982. Consequently, possible differences in the serotype of the plaque bacterium being tested compared to the antigens source used in the ELISA may have caused a decrease in the detection of total antibody against a specific periodontal pathogen.…”

Section: Discussionmentioning

confidence: 99%

Clinical, microbiological and immunological characteristics of subjects with “refractory” periodontal disease

Magnusson

Marks

Clark

et al. 1991

J Clinic Periodontology

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The aim of the present study was to analyze the relationship between attachment loss and clinical, microbiological, and immunological parameters in a group of 21 human subjects exhibiting poor response to previous periodontal therapy. All had been treated with periodontal surgery, tetracycline, and subsequent maintenance recalls to periodontists who, upon detection of disease progression, referred the subjects to our clinic. In our clinic, each subject received oral hygiene instruction and a thorough subgingival scaling and root planing utilizing as many appointments as necessary. Clinical indices, including gingival index, bleeding on probing, suppuration, plaque index, pocket depth, and duplicate measurements of attachment level from an acrylic stent, were collected at monthly intervals. Probing measurements were performed using the Florida Probe. When significant attachment loss (0.8-1.2 mm) was detected in at least 1 site, a bacterial sample was taken from that site and from a comparably deep, but non-progressing, control site. Microbial samples were enumerated by darkfield microscopy, on selective and non-selective media, and by predominant cultivable technique. Blood samples were also collected to determine antibody levels against potential pathogens. There was no difference in the amount of plaque present in sites gaining or losing attachment, but losing sites exhibited more bleeding and suppuration. 20 of the 21 subjects were tested; of these, 17 exhibited elevated serum antibody against one or more of the following microorganisms: Actinobacillus actinomycetemcomitans, Bacteroides, gingivalis, and Eikenella corrodens. However, few, if any, of the "classical" pathogens were detected in the plaque samples obtained at the time progressive disease was diagnosed. The only exception was Streptococcus intermedius, which occurred in slightly higher numbers in active sites.

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Section: Discussionmentioning

confidence: 99%

Clinical, microbiological and immunological characteristics of subjects with “refractory” periodontal disease

Magnusson

Marks

Clark

et al. 1991

J Clinic Periodontology

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“…These findings have also been confirmed by the routine detection of significant elevations in serum antibody coincident with the infection (Listgarten et al 1981, Ebersole et al 1982, Genco et al 1985, Tew et al 1985b, Gunsolley et al 1988, Ebersole 1990, Mooney et al 1993, Ebersole & Taubman 1994. More detailed investigations of this antibody response have delineated some antigenic specificity for various membrane proteins (Califano et al 1989, Watanabe et al 1989, Bolstad et al 1990, Sims et al 1991, Wilson 1991a, b, Saito et al 1993, Ebersole et al 1995a, Nakano et al 1995, carbohydrates (Califano et al 1989), and lipopolysaccharide epitopes , Wilson & Schifferle 1991, Wilson & Hamilton 1992. The majority of these reports described cross-sectional analyses of antibody responses in rather heterogeneous populations of periodontitis patients.…”

mentioning

confidence: 86%

Longitudinal human serum antibody responses to outer membrane antigens of Actinobacillus actinomycetemcomitans

Ebersole¹,

Steffen²,

Cappelli³

1999

J Clinic Periodontology

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We hypothesize that serum antibody responses to antigens of a periodontopathogen would vary temporally and that the specificity of these host antibodies would relate to infection and disease activity. To test the hypothesis, we obtained between 6 and 13 serum samples from Actinobacillus actinomycetemcomitans (Aa)-positive (serological and microbiological) periodontitis patients at time points between 18 and 42 months into the study, and evaluated specific antibody responses to outer membrane antigens (OMA) of Aa strain Y4. Sera from these patients detected 22 different OMA. Early-onset (EOP; n=7) and adult (AP; n=11) periodontitis patients responded to 35% and 41% of the OMA, respectively. 2 of 9 sera from healthy subjects detected no antigens and 7/9 sera detected 7% of the OMA (p<0.0001 versus EOP and AP). The frequency of antibody responses to the 17 kDa antigen were similar between diseased, infected patients and the uninfected, normal subjects, suggesting that it may be stimulated as a cross-reactive antigen. Antibody to the 28, 38, and 90 kDa antigens were significantly more common in diseased patients (>90%) versus normal subjects (p<0.01, p<0.002, and p<0.002, respectively) and were unique among diseased, infected patients, which may be indicative of infections with Aa. A 65 kDa antigen showed an increased frequency of reaction in the AP versus the EOP (p=0.01) patients, which exemplified a potential distinction in response to Aa between adult and early-onset disease classifications. Seventeen of 22 OMA could be detected in every sample from at least one patient. Longitudinal samples from seropositive EOP and AP reacted 80-100% of the time with the 17, 28, and 100 kDa antigens. Finally, five antigens of 15, 38, 58, 65, and 79 kDa were detected in 33-83% of the seropositive patients; however, antibody to these OMA reacted variably at different sampling points, suggesting some antigenic response diversity over time.

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“…Various investigators have demonstrated that patients with localized juvenile periodontitis exhibit elevated serum IgG antibody to A. actinomycetemcomitans and that the immunodominant antigen of the organism is a capsular-like polysaccharide or the polysaccharide portion of lipopolysaccharide (6,10,23,26,36). In addition, serum IgG antibody to A. actinomycetemcomitans elevated in periodontitis patients has been reported to enhance in vitro phagocytosis of the organism by human neutrophils (3,27,34).…”

Section: Discussionmentioning

confidence: 99%

“…The serotype-specific antigens of serotypes d and e have not yet characterized. Patients with localized juvenile periodontitis exhibit elevated serum antibody levels to SPA of A. actinomycetemcomitans serotype b (6,23,26,36). However, the role of serum SPA-specific antibodies in host responses to A. actinomycetemcomitans is poorly understood.…”

Section: Actinobacillusmentioning

confidence: 99%

Immunogenicity of Actinobacillus actinomycetemcomitans serotype b‐specific polysaccharide‐protein conjugate

Takamatsu-Matsushita

Yamaguchi

Kawasaki

et al. 1996

Oral Microbiology and Immunology

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To enhance the immunogenicity of capsular-like serotype b-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans, the purified antigen was coupled with bovine serum albumin via an adipic acid hydrazide functional group. The conjugate (SPA-bovine serum albumin) or the native SPA was administered subcutaneously or intranasally to BALB/c mice. Neither subcutaneous immunization with SPA emulsified in Freund adjuvant nor intranasal immunization with SPA and cholera toxin B subunit elicited any antibody responses to the polysaccharide antigen. High serum immunoglobulin M (IgM) and IgG responses to SPA were induced by subcutaneous immunization with SPA-bovine serum albumin in Freund adjuvant. Serum and salivary IgA responses to SPA in addition to IgM and IgG responses were induced by intranasal immunization with the conjugate and cholera toxin B subunit. To investigate the functional activity of SPA-specific antibodies, the opsonic activity of sera from BALB/c mice immunized with the conjugate was assessed by chemiluminescence assay using human polymorphonuclear leukocytes. Murine antisera efficiently opsonized A. actinomycetemcomitans serotype b in the assay, suggesting that antibodies to SPA of the organism might serve as a protective role.

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Immunodominant antigen of Actinobacillus actinomycetemcomitans Y4 in high-responder patients

Cited by 82 publications

References 24 publications

Clinical, microbiological and immunological characteristics of subjects with “refractory” periodontal disease

Clinical, microbiological and immunological characteristics of subjects with “refractory” periodontal disease

Longitudinal human serum antibody responses to outer membrane antigens of Actinobacillus actinomycetemcomitans

Immunogenicity of Actinobacillus actinomycetemcomitans serotype b‐specific polysaccharide‐protein conjugate

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