IMMUNODIFFUSION STUDIES ON <i>ESCHERICHIA COLI</i>

Antibodies against Escherichia coli O antigen from rabbits immunized with formalin-killed bacteria were tested for cytotoxic capacity in an antibody-dependent cell-mediated cytotoxicity (ADCC) assay with human lymphocytes as effector cells and autologous papainized erythrocytes coated with O antigen as target cells. The cytotoxic titres were compared with the titres obtained with three methods of antibody quantitation. It was found that ADCC recorded antibodies with similar sensitivity as the enzyme-linked immunosorbent assay (ELISA) for IgG, but was much more sensitive than the ammonium sulphate precipitation (ASP) and indirect haemagglutination (IHA) using beta-mercaptoethanol reduced sera. The ADCC titres were found to correlate very well with the titres obtained with ASP, ELISA and IHA for IgG but not for IgM, which is in accordance with a previous notion that ADCC is primarily mediated via IgG antibodies. ADCC should be considered as a possible immunopathologic mechanism in renal parenchymal damage in connection with urinary tract infections.

show abstract

Antibody-dependent cell-mediated cytotoxicity againstEscherichia coli O antigens

Hagberg

Ahlstedt

Hanson

1982

Eur. J, Clin. Microbiol.

View full text Add to dashboard Cite

show abstract

Antibodies to endotoxin from Vibrio cholerae measured by bactericidal and indirect hemagglutination tests. Differences in immunoglobulin classes and specificity

Holmgren

Svennerholm

Ouchterlony

1972

Journal of Immunological Methods

View full text Add to dashboard Cite

Studies on Escherichia coli vaccines: Development of an agar-gel immunodiffusion method for the identification of antigens in E. coli vaccines

Hussaini

Edgar

1981

Journal of Biological Standardization

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

IMMUNODIFFUSION STUDIES ON ESCHERICHIA COLI

Cited by 11 publications

References 18 publications

Antibody-dependent cell-mediated cytotoxicity againstEscherichia coli O antigens

Antibody-dependent cell-mediated cytotoxicity againstEscherichia coli O antigens

Antibodies to endotoxin from Vibrio cholerae measured by bactericidal and indirect hemagglutination tests. Differences in immunoglobulin classes and specificity

Studies on Escherichia coli vaccines: Development of an agar-gel immunodiffusion method for the identification of antigens in E. coli vaccines

Contact Info

Product

Resources

About