Immunodiagnostics for Cherry virus A and Cherry necrotic rusty mottle virus

Noorani, Salik; Awasthi, Poorwa; Sukapaka, Mahesh; Singh, Latika; Ram, Raja; Sharma, Manju; Zaidi, A. A.; Hallan, Vipin

doi:10.1007/s13562-013-0240-8

Cited by 9 publications

(10 citation statements)

References 53 publications

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“…DAS‐ELISA was carried out using the indigenously raised antisera against the CP of CNRMV (Noorani et al. ). To further confirm the results obtained from DAS‐ELISA, the samples were tested by dot blot hybridization.…”

Section: Methodsmentioning

confidence: 99%

“…To characterize the coat protein gene of CNRMV from infected bamboo, two ELISA and RT‐PCR positive (with detection primers) samples were taken and analysed using the CP primers (Noorani et al. ), under similar set of conditions as described earlier. Positive amplification of ~841 nt was cloned and sequenced and submitted to the GenBank database, and the accession numbers and were obtained.…”

Section: Methodsmentioning

confidence: 99%

“…In context of our research on cherry viruses, we characterized a sweet cherry isolate JK10 of CNRMV from the Jammu and Kashmir region, and the CP gene of this isolate was used for developing antisera‐based immunodiagnostics for CNRMV which was the first report of immunodiagnostics for CNRMV from India (Noorani et al. ). Using the indigenously raised antisera, the incidence of CNRMV in sweet cherries in India was found to be 46%.…”

Section: Introductionmentioning

confidence: 99%

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Molecular evidence for bamboo as a new natural host of Cherry necrotic rusty mottle virus

et al. 2014

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Summary Himalayan ‘maggar’ bamboo (Dendrocalamus hamiltonii), widely distributed in the Himalayas, was found to exhibit mild virus‐like symptoms, which included mosaic, chlorosis, necrotic spots and mottling. Leaf samples from symptomatic plants were subjected to reverse transcription polymerase chain reaction (RT‐PCR) for the detection of Cherry necrotic rusty mottle virus (CNRMV). Sequencing of the amplicon confirmed the presence of the virus. To further establish bamboo as a natural host of CNRMV, 35 varieties of bamboos maintained at CSIR‐IHBT were subjected to double‐antibody sandwich enzyme linked immunosorbent assay (DAS‐ELISA) and dot blot hybridization. Our results showed the presence of CNRMV in 21 species of bamboos belonging to five genera, viz. Bambusa, Dendrocalamus, Fargesia, Arundinaria and Melocanna. For further confirmation of the results, the coat protein (CP) gene of CNRMV was amplified from two different local ‘maggar’ plants. Sequencing of the CP gene confirmed the presence of the virus. The two isolates characterized from bamboo plants, shared 99.8% nucleotide sequence identity with sweet cherry isolate JK10 characterized from India. This study presents sequence information from two isolates of the virus. CNRMV infection in bamboo is being reported for the first time and only the second virus reported in bamboo after Bamboo mosaic virus (BaMV).

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular evidence for bamboo as a new natural host of Cherry necrotic rusty mottle virus

et al. 2014

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“…This virus has also been detected in apricot, peach, plum and sour cherry plants in China (Zhou et al , ). In India CNRMV has been detected in sweet cherry (Noorani et al , ) and 21 out of 35 tested varieties of bamboos (Awasthi et al , ).…”

Section: Introductionmentioning

confidence: 99%

Himalayan wild cherry (Prunus cerasoides) as a new natural host of Cherry necrotic rusty mottle virus (CNRMV) and a possible role of insect vectors in their transmission

Awasthi

Ram

Reddy

et al. 2015

Annals of Applied Biology

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A Himalayan wild cherry (Hwc) tree (Prunus cerasoides) growing in the vicinity of an already reported Cherry necrotic rusty mottle virus (CNRMV) infected bamboo (Dendrocalamus hamiltonii) showed severe virus-like symptoms on the leaves consisting of marginal chlorosis, mosaic, shot holes and necrotic spots. Reverse transcription-polymerase chain reaction (RT-PCR) using virus specific primers confirmed the presence of the virus. In order to assess the prevalence of this virus, 40 symptomatic and asymptomatic Hwc plants along with four different suspected insect vectors were analysed by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA), dot blot hybridisation and RT-PCR. CNRMV was detected in 57% of the wild cherry samples, two aphid species Astegopteryx bambusae and Tinocalloides montanus, and in a delphacid of Bambusiphaga sp. Complete coat protein (CP) gene of CNRMV was sequenced from two randomly selected samples. The isolates characterised from Hwc shared 99% similarity to the already characterised isolates of CNRMV from India, suggesting a conserved nature of the CP gene. This study represents the first report of CNRMV infection in Himalayan wild cherry and its possible insect vectors.

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“…Reduced growth of infected trees, significant decrease in yield, and early death of trees have also been reported (see the review by . The virus exhibits mainly sporadic or restricted distribution and has been reported from various European countries, North America, Chile, Japan, Korea, and New Zealand (Fry & Wood 1973;Isogai et al 2004;Sabanadzovic et al 2005;Fiore & Zamorano 2013;Noorani et al 2013;Zhou et al 2013;Cho et al 2014;EPPO 2016a (Li & Mock 2008;Zhou et al 2013;Zindovic et al 2014). CNRMV has been detected in what probably were naturally infected wild roses and bamboos, and its natural spread between hosts by specific Asian aphids has recently been proposed but not proven in India (Awasthi et al 2015a, b).…”

mentioning

confidence: 99%

Cherry necrotic rusty mottle and Cherry green ring mottle viruses in Czech cherry germplasm

Špak¹,

Přibylová²,

Šafářová³

et al. 2017

Plant Prot. Sci.

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AbstractŠpak J., Přibylová J., Šafářová D., Lenz O., Koloniuk I., Navrátil M., Fránová J., Špaková V., Paprštein F. (2017) Using reverse transcription polymerase chain reaction, 160 sweet and sour cherry trees from a germplasm collection, orchards, and wild trees in the Czech Republic were screened for the presence of Cherry necrotic rusty mottle virus (CNRMV) and Cherry green ring mottle virus (CGRMV). The viruses were detected exclusively in sweet cherry trees in the germplasm collection, with CNRMV determined in two trees and CGRMV in four trees. Using next-generation sequencing, nearly complete genomic sequences (complete ORFs) were obtained for one CNRMV and three CGRMV isolates. Their relatedness to GenBank sequences of isolates from different countries together with negative results from screening outside of the germplasm collection suggests that the viruses had been imported with accessions.

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Immunodiagnostics for Cherry virus A and Cherry necrotic rusty mottle virus

Cited by 9 publications

References 53 publications

Molecular evidence for bamboo as a new natural host of Cherry necrotic rusty mottle virus

Molecular evidence for bamboo as a new natural host of Cherry necrotic rusty mottle virus

Himalayan wild cherry (Prunus cerasoides) as a new natural host of Cherry necrotic rusty mottle virus (CNRMV) and a possible role of insect vectors in their transmission

Cherry necrotic rusty mottle and Cherry green ring mottle viruses in Czech cherry germplasm

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