Immunodiagnostic potential of<i>Wuchereria bancrofti</i>L1 antigen–based filarial immunoglobulin G4 detection assay

Prasad, B. K.; Khatri, Vishal; Yadav, PragyaD; Chandra, Mithilesh; Lakshmi, Degati Vijaya; Goswami, Kalyan

doi:10.1093/trstmh/try110

Cited by 1 publication

(3 citation statements)

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“…These proteins are characterized by numerous invariant positions organized into defined motifs [ 30 ]. They are best known as potent immunogens and for their importance in diagnosis [ 19 , 30 – 33 ]. Although the antigens detailed here appear to be variants of one or a few closely related proteins from B .…”

Section: Recombinant Antigensmentioning

confidence: 99%

“…bancrofti L3 cDNA expression library. WbL1 seems to be the same antigen as Wb14 with a single amino acid substitution at position 130, glutamine to leucine [ 33 ].…”

Section: Recombinant Antigensmentioning

confidence: 99%

“…This multicentric evaluation displayed a maximum sensitivity of 93% and specificity of 98%. The ELISA anti-WbL1 IgG4 was then proposed as a new optional test for initial screening and epidemiological surveys of filarial infections in LF endemic areas [ 33 ].…”

Section: Diagnostic Testsmentioning

confidence: 99%

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Recombinant antigens used as diagnostic tools for lymphatic filariasis

et al. 2021

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Lymphatic filariasis (LF) is a parasitic disease caused by the worms Wuchereria bancrofti, Brugia malayi, or Brugia timori. It is a tropical and subtropical illness that affects approximately 67 million people worldwide and that still requires better diagnostic tools to prevent its spread and enhance the effectiveness of control procedures. Traditional parasitological tests and diagnostic methods based on whole protein extracts from different worms are known for problems related to sample time collection, sensitivity, and specificity. More recently, new diagnostic tools based on immunological methods using recombinant antigens have been developed. The current review describes the several recombinant antigens used as tools for lymphatic filariasis diagnosis in antigen and antibody capture assays, highlighting their advantages and limitations as well as the main commercial tests developed based on them. The literature chronology is from 1991 to 2021. First, it describes the historical background related to the identification of relevant antigens and the generation of the recombinant polypeptides used for the LF diagnosis, also detailing features specific to each antigen. The subsequent section then discusses the use of those proteins to develop antigen and antibody capture tests to detect LF. So far, studies focusing on antibody capture assays are based on 13 different antigens with at least six commercially available tests, with five proteins further used for the development of antigen capture tests. Five antigens explored in this paper belong to the SXP/RAL-2 family (BmSXP, Bm14, WbSXP-1, Wb14, WbL), and the others are BmShp-1, Bm33, BmR1, BmVAH, WbVAH, BmALT-1, BmALT-2, and Wb123. It is expected that advances in research with these antigens will allow further development of tests combining both sensitivity and specificity with low costs, assisting the Global Program to Eliminate Lymphatic Filariasis (GPELF).

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Section: Recombinant Antigensmentioning

confidence: 99%

“…bancrofti L3 cDNA expression library. WbL1 seems to be the same antigen as Wb14 with a single amino acid substitution at position 130, glutamine to leucine [ 33 ].…”

Section: Recombinant Antigensmentioning

confidence: 99%