Immunodiagnosis of virulent strains of Aeromonas hydrophila associated with epizootic ulcerative syndrome (EUS) using a monoclonal antibody

Abstract

A virulent strain of Aeromonas hydrophila associated with epizootic ulcerative syndrome (EUS) was used to produce monoclonal antibodies that identified virulent strains of A. hydrophila. Antibodies from a clone, designated as F26P5C8, were found to identify the A. hydrophila serotype I isolates associated with EUS fish, and which were found to be virulent after subsequent inoculation studies. Immunodiagnosis of a large number of A. hydrophila from Australia and Japan showed some additional isolates to be identified by F26P5C8, but the status of their virulence is presently unknown.

Simple and direct detection of Aeromonas hydrophila infection in the goldfish, Carassius auratus (L.), by dot blotting using specific monoclonal antibodies

“…MAbs specific to A. hydrophila (groups 6 and 7) could recognize most of the A. hydrophila isolates obtained from both patients and aquatic animals, except for one isolate, AH7 (obtained from a patient), that displayed distinct biochemical reactions and immunoreactivities (Table 3). In the previous two reports, only one MAb was present; the MAb F26P5C8, specific to the LPS of A. hydrophila , which recognized only some virulent and non‐virulent isolates of A. hydrophila isolated from diseased and healthy fish from Malaysia and the Philippines (12/34 isolates), Japan (2/34 isolates), Australia (10/70 isolates) and human diarrhoea (4/9 isolates) (Cartwright et al. 1994).…”

“…This study demonstrated that these MAbs can be used for the simple and direct detection of A. hydrophila and Aeromonas spp. in infected fish by dot blotting without sample treatment or isolation of the bacteria, as required in an ELISA (Cartwright et al. 1994; Delamare et al.…”

“…However, PAbs may give false‐positive results in addition to non‐specific background staining. By contrast, monoclonal antibodies (MAbs) specific to A. hydrophila have been generated against the lipopolysaccharide (LPS) of A. hydrophila type I isolates (Cartwright, Chen, Hanna, Gudkovs & Tajima 1994). A MAb against the 110 ‐kDa protein of A. hydrophila with low cross‐reactivity to other Aeromonas spp.…”