Immunodiagnosis of toxocarosis in humans: evaluation of a new enzyme-linked immunosorbent assay kit

Jacquier, P; Gottstein, Bruno; Stingelin, Yvonne; Eckert, Johannes

doi:10.1128/jcm.29.9.1831-1835.1991

Cited by 172 publications

(73 citation statements)

References 23 publications

(18 reference statements)

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“…Medicine comprised 64.4% articles, followed by Immunology and Microbiology with 36.1%, and Veterinary with 13.1%. Table 4 presents the 20 most commonly cited toxocariasis publications between 1932 and 2015 [33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52]. The IFs varied from 1.151 for the 4th most cited paper to 45.217 for the 9th most cited paper.…”

Section: Resultsmentioning

confidence: 99%

Global toxocariasis research trends from 1932 to 2015: a bibliometric analysis

Zyoud

2017

Health Res Policy Sys

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BackgroundToxocariasis is a highly prevalent parasitic disease in the tropical regions of the world, with its impact on public health being typically underestimated. To better recognise the trends and characteristics of toxocariasis research, this study is a bibliometric analysis of the global toxocariasis research.MethodsSearches were completed on April 5, 2016, using the Scopus database. A search without any language restriction was performed to extract publications dealing with toxocariasis. Terms related to toxocariasis were used to perform a title keyword search.ResultsA total of 2765 publications comprising 11 document types and published between 1932 and 2015 were included in the analysis. Articles were the most popular document form, accounting for 83.62% of all publications, followed by letters (3.80%) and reviews (3.4%). The annual number of research publications increased from 30 in 1980 to 111 in 2015, indicating that the number of publications on toxocariasis has increased slowly over the past 35 years. The United States of America and Japan are the predominant countries of origin, with 303 articles and 207 articles, respectively, followed by Brazil and the United Kingdom, with 180 (6.5%) each. The h-index for all the publications was 60. The highest h-index were for publications from the United Kingdom (h-index value = 43) and the United States (h-index value = 39); these two countries were also involved with the highest number of international collaborations, with 27 and 28 countries, respectively.ConclusionsDeveloped countries, including the United States, Japan, the United Kingdom, France, Germany and Italy, are the world’s leaders in toxocariasis research, contributing to more than 34% of the total published literature. In addition, developing countries, such as Brazil, Poland, Argentina and India, showed a noticeable increase in published papers on toxocariasis research in recent years. A push for more collaboration is needed to achieve a superior research strategy related to toxocariasis at the global level from the viewpoint of epidemiological data, clinical aspects, medical ecology, molecular aspects and treatment practices associated with toxocariasis.

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Section: Resultsmentioning

confidence: 99%

Global toxocariasis research trends from 1932 to 2015: a bibliometric analysis

Zyoud

2017

Health Res Policy Sys

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“…The lack of better performing and more suitable tests for diagnostic purposes has led to this situation. Jacquier et al 15 reported that the IgG-ELISA kit has a specificity and sensitivity of 86% and 91%, respectively. This assay gives false-positive reactions with non-T. canis helminthic infections that are commonly found in tropical settings.…”

Section: Discussionmentioning

confidence: 99%

A Lateral Flow Rapid Test for Human Toxocariasis Developed Using Three Toxocara canis Recombinant Antigens

Yunus

Farrizam

Karim

et al. 2018

The American Journal of Tropical Medicine and Hygiene

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Laboratory diagnosis of toxocariasis is still a challenge especially in developing endemic countries with polyparasitism. In this study, three recombinant antigens, rTES-26, rTES-30, and rTES-120, were expressed and used to prepare lateral flow immunoglobulin G4 (IgG4) dipsticks. The concordance of the results of the rapid test (comprising three dipsticks) with a commercial IgG-enzyme-linked immunosorbent assay (ELISA) (Cypress Diagnostics, Belgium) was compared against the concordance of two other commercial IgG-ELISA kits (Bordier, Switzerland and NovaTec, Germany) with the Cypress kit. Usingpositive samples, the concordance of the dipstick dotted with rTES-26, rTES-30, and rTES-120 was 41.4% (12/29), 51.7% (15/29), and 72.4% (21/29), respectively. When positivity with any dipstick was considered as an overall positive rapid test result, the concordance with the Cypress kit was 93% (27/29). Meanwhile, when compared with the results of the Cypress kit, the concordance of IgG-ELISA from NovaTec and Bordier was 100% (29/29) and 89.7% (26/29), respectively. Specific IgG4 has been recognized as a marker of active infection for several helminthic diseases; therefore, the two non-concordant results of the rapid test when compared with the NovaTec IgG-ELISA kit may be from samples of people with non-active infection. All the three dipsticks showed 100% (50/50) concordance with the Cypress kit when tested with serum from individuals who were healthy and with other infections. In conclusion, the lateral flow rapid test is potentially a good, fast, and easy test for toxocariasis. Next, further validation studies and development of a test with the three antigens in one dipstick will be performed.

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“…The T. canis ELISA used an excretory/secretory (ES) antigen from Toxocara larvae to screen for serum immunoglobulin G (IgG) antibodies. The Toxocara ES antigen-based ELISA for the detection of IgG antibodies was 78-91% sensitive and 86-93% specific for the diagnosis of toxocariasis (Speiser & Gottstein, 1984;Jacquier et al, 1991). The results were read using an ELISA reader at 450/650-620 nm.…”

Section: Blood Sampling and Serologic Evaluationsmentioning

confidence: 99%

Association between epilepsy and cysticercosis and toxocariasis: A population‐based case–control study in a slum in India

Singh

Bawa

Chinna³

et al. 2012

Epilepsia

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SUMMARYPurpose: To assess the association between epilepsy and exposure to the parasites, Toxocara canis and Taenia solium in a slum-community in India. Methods: A door-to-door survey to determine the prevalence of epilepsy was carried out by trained field workers. For every case, one age-and gender-matched control was selected from the same community. Serologic evaluation was carried out to detect antibodies against T. canis and T. solium. Key Findings: The crude prevalence of active epilepsy was 7.2 per 1,000. We enrolled 114 people with active epilepsy and 114 controls. The prevalence of antibodies to T. canis was similar in people with active epilepsy (4.7%; 5 of 106 people) and in controls (5.7%; 6 of 106 people). The prevalence of antibodies to T. solium was 25.5% (27 of 106) in people with active epilepsy, significantly higher than in controls (12.3%; 13 of 106 cases; p = 0.02). Adjusted conditional (fixed-effects) logistic regression estimated an odds ratio of 2.8 (95% confidence interval 1.2-6.8) for detection of T. solium antibodies. Nineteen people with active epilepsy demonstrated evidence of neurocysticercosis (NCC) on magnetic resonance imaging (MRI), including 7 (36.5%) with solitary cysticercus granuloma. Significance: Our findings do not support an association between epilepsy and exposure to T. canis in the community studied. A significant association between T. solium exposure and epilepsy was observed. Of those with active epilepsy and evidence of NCC on MRI, a large proportion demonstrated solitary cysticercus granuloma.

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Immunodiagnosis of toxocarosis in humans: evaluation of a new enzyme-linked immunosorbent assay kit

Cited by 172 publications

References 23 publications

Global toxocariasis research trends from 1932 to 2015: a bibliometric analysis

Global toxocariasis research trends from 1932 to 2015: a bibliometric analysis

A Lateral Flow Rapid Test for Human Toxocariasis Developed Using Three Toxocara canis Recombinant Antigens

Association between epilepsy and cysticercosis and toxocariasis: A population‐based case–control study in a slum in India

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