Immunodiagnosis of Prune dwarf virus using antiserum produced to its recombinant coat protein

Preparation of Polyclonal Antibodies to Grapevine fanleaf Virus Coat Protein Expressed in Escherichia coli

“…Proteins from several plant viruses have been produced in E. coli and used for raising virus-specific antibodies for immunodiagnosis (Lee and Chang, 2008;Cerovska et al, 2006;Jain et al, 2005;Abou-Jawdah et al, 2004;Cerovska et al, 2003;Hourani and Abou-Jawdah, 2003;Korimbocus et al, 2002;Kumari et al, 2001). In this study, we report the use of GFLV CP expressed in E. coli as the antigen to produce GFLV-specific polyclonal antibodies, which proved to be efficient, when tested in ELISA and Western blotting.…”

“…Antibodies against recombinant proteins of a number of plant viruses have been prepared successfully (Iracheta-Cardenas et al, 2008;Lee and Chang, 2008;Raikhy et al, 2007;Jain et al, 2005;Abou-Jawdah et al, 2004;Nickel et al, 2004;Cerovska et al, 2003;Hourani and Abou-Jawdah, 2003;Korimbocus et al, 2002;Kumari et al, 2001). By the use of different IPTG concentrations it was concluded that the optimal expression was achieved, when transformed E. coli Rosetta cells was induced with 1 mM IPTG at OD = 0.5 and grown at 37°C for 4 h. Different IPTG concentrations including 1 mM (Bragard et al, 2000;Kadkhodayan et al, 2000;Saini and Vrati, 2003;Thomas and Baneyx, 1996) and 0.4 mM (Jacob and Usha, 2002) have been reported as the optimal concentration with various genes of interest for expression of Cardamom mosaic virus CP and 0.1 mM (Petrzik et al, 2001) for that of Prunus necrotic Ring Spot virus (PNRSV) CP in E. coli.…”

Serological Methods to Confirm Expression of Coat Protein Gene From an Iranian Isolate of Cucumber Mosaic Virus in Escherichia coli

“…E. coli as a prokaryotic system because of its high expression capacities of many heterologous proteins, low cost, efficient generation time and fast high-density cultivation is known as a successful host. Accordingly, expression of CP gene from numerous plant viruses has beensuccessfully done in E. coli (18)(19)(20)(21). Apart from the pET vector system, a number of other vector systems such as pGEX (22,23), pTrcHis (24) and pTBG (H) (25) have also been used for expression of foreign genes in E. coli.…”

Immunological Comparisons of Polyclonal Antibodies Produced Against the Recombinant Coat Protein and Viral Particles for Detecting Egyptian Banana- Cucumber Mosaic Cucumovirus

“…Also, the results indicated that fusion CP maintains its native antigenicity and specificity, providing a good source of antigen in preparation of Ban-CMV related antibodies. There are numerous reports of the expression of plant viruses CP in E. coli and use as an antigen, instead of virus particles (Abou-Jawdah et al, 2004;Fajardo et al, 2007;Hema et al, 2008;Salimi et al, 2012;Shi et al, 2013).…”