Immunodiagnosis of Human Fascioliasis by an Enzyme-Linked Immunosorbent Assay (ELISA) and a Micro-ELISA

Carnevale, Silvana; Rodrı́guez, Mónica I.; Santillán, Graciela; Labbé, Jorge H.; Cabrera, Marta; Bellegarde, Enrique J.; Velásquez, Jorge Néstor; Trgovcic, Jorge E.; Guarnera, Eduardo

doi:10.1128/cdli.8.1.174-177.2001

Cited by 54 publications

(48 citation statements)

References 18 publications

(10 reference statements)

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“…Several ELISA techniques have been described for the serodiagnosis of fascioliasis, and most rely on excretory-secretory products (11,15) or recombinant proteases (11,34) that are the major components of ESPs. In the present study, we demonstrated that a cytosolic ferritin-like protein (FhFtn-1) and a tegument-associated protein (FhTP16.5) are also excellent antigens for the serodiagnosis of chronic fascioliasis.…”

Section: Discussionmentioning

confidence: 99%

“…The antigens traditionally used in serological tests are crude extracts or excretory-secretory products (ESPs) of F. hepatica (11,15,16). Several F. hepatica purified antigens (17,18) and recombinant antigens have been employed to enhance the specificity of diagnostic assays (11,19,20).…”

mentioning

confidence: 99%

“…(8). Other serological techniques in which specific antibodies are detected, including a dot blot assay (9), lateral flow immunoassay (10), or indirect enzyme-linked immunosorbent assay (ELISA) (11)(12)(13), have been studied. Detection of antibodies in serum by ELISA is a frequently used diagnostic tool, is considered a sensitive and reliable means of diagnosing acute infections, and can be used as an adjunct to fecal analysis for the diagnosis of latent and chronic infections (14).…”

mentioning

confidence: 99%

“…Several F. hepatica purified antigens (17,18) and recombinant antigens have been employed to enhance the specificity of diagnostic assays (11,19,20). The most notable are cathepsin L, the major protease involved in F. hepatica virulence (21), fatty acid-binding proteins (FABPs) (22)(23)(24), and saposin-like proteins (FhSAP2) (25), which have been documented as useful immunodiagnostic antigens for serological detection of fascioliasis.…”

mentioning

confidence: 99%

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Development of Two Antibody Detection Enzyme-Linked Immunosorbent Assays for Serodiagnosis of Human Chronic Fascioliasis

et al. 2014

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Coprological examination based on egg detection in stool samples is currently used as the gold standard for the diagnosis of human fascioliasis. However, this method is not effective during the acute phase of the disease and has poor sensitivity during the chronic phase. Serodiagnosis has become an excellent alternative to coprological examination in efforts to combat the effects of fascioliasis on human and animal health. Two novel recombinant Fasciola hepatica proteins, i.e., a ferritin (FhFtn-1) and a tegument-associated protein (FhTP16.5), were used as antigens to develop in-house enzyme-linked immunosorbent assay (ELISA) methods. The assays were optimized and validated using 152 serum samples from humans with a known infection status, including healthy subjects, patients with chronic fascioliasis, and patients with other parasitic diseases. The FhFtn-1 ELISA was shown to be 96.6% sensitive and 95.7% specific; the respective parameters for the FhTP16.5 ELISA were 91.4% and 92.4%. The performances of the FhFtn-1 and FhTP16.5 ELISAs were compared with that of an available commercial test (the DRG test) using a subset of serum samples. Our in-house tests were slightly more sensitive than the DRG test in detecting antibodies against F. hepatica, but the differences were not statistically significant. In conclusion, the present study provides evidence for the potential of the FhFtn-1 and FhTP16.5 ELISAs as diagnostic tools for human fascioliasis, as might be implemented in conjunction with standard assays for large-scale screenings in areas where the disease is endemic and for the detection of occasional cases in clinical laboratories.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Development of Two Antibody Detection Enzyme-Linked Immunosorbent Assays for Serodiagnosis of Human Chronic Fascioliasis

et al. 2014

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“…FAST-ELISA has a sensitivity rate of 95%, although the exact specificity has not been determined. 10,11 Triclabendazole has been shown to be very effective against fasciola hepatica infections. The drug is administered at a dose of 10 mg/kg for 1 or 2 days, and it is well-tolerated.…”

Section: Discussionmentioning

confidence: 99%

Hepatic Mass Caused by Fasciola Hepatica: A Tricky Differential Diagnosis

Yılmaz¹,

Köklü²,

Gedikoğlu³

2013

The American Society of Tropical Medicine and Hygiene

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Abstract. Fascioliasis is a zoonotic infestation caused by the trematode fasciola hepatica. It presents a wide spectrum of clinical pictures ranging from fever and eosinophilia to ambiguous gastrointestinal symptoms in the acute phase. However, it may often be overlooked, especially in the acute phase, because of uncertain symptoms. Fasciola hepatica can have an initial presentation similar to the presentation of malignancy. Here, we report a case of a hepatic mass caused by fasciola hepatica.

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Foodborne Trematodes

Adams¹

Foodborne Parasites

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Immunodiagnosis of Human Fascioliasis by an Enzyme-Linked Immunosorbent Assay (ELISA) and a Micro-ELISA

Cited by 54 publications

References 18 publications

Development of Two Antibody Detection Enzyme-Linked Immunosorbent Assays for Serodiagnosis of Human Chronic Fascioliasis

Development of Two Antibody Detection Enzyme-Linked Immunosorbent Assays for Serodiagnosis of Human Chronic Fascioliasis

Hepatic Mass Caused by Fasciola Hepatica: A Tricky Differential Diagnosis

Foodborne Trematodes

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