Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae)

Gawecki, Robert; Sala, Katarzyna; Kurczyńska, Ewa; Świątek, Piotr; Płachno, Bartosz J.

doi:10.1007/s00709-016-0980-0

Cited by 15 publications

(20 citation statements)

References 54 publications

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“…This supports the likely conservation of differentiation processes during sexual and asexual female gametophyte maturation suggested from recent observations in mature female gametophytes of apomictic Taraxacum spp. and strawberry using antibodies to detect AGP epitopes (Gawecki et al, 2017;Leszczuk and Szczuka, 2018) and our previous observations comparing reporter gene expression in maturing sexual and aposporous female gametophytes in Hieracium (Tucker et al, 2003).…”

Section: Ai and Fm Cell Contact Precedes Megaspore Demise And Mitoticmentioning

confidence: 71%

“…; Dziadczyk et al, 2011;Leszczuk and Szczuka, 2018). Arabinogalactan proteins also are detected in maturing asexual female gametophytes of apomictic Taraxacum spp., which develop by mitotic diplospory (Gawecki et al, 2017). Despite several studies involving arabinogalactan proteins, the underlying mechanisms of their function remain unclear, and a range of models have been proposed (Ellis et al, 2010;Lamport and Várnai, 2013;Lamport et al, 2018).…”

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confidence: 99%

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Asexual Female Gametogenesis Involves Contact with a Sexually-Fated Megaspore in Apomictic Hieracium

et al. 2018

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Apomixis results in asexual seed formation where progeny are identical to the maternal plant. In ovules of apomictic species of the subgenus, meiosis of the megaspore mother cell generates four megaspores. Aposporous initial (AI) cells form during meiosis in most ovules. The sexual pathway terminates during functional megaspore (FM) differentiation, when an enlarged AI undergoes mitosis to form an aposporous female gametophyte. Then, the mitotically programmed FM dies along with the three other megaspores by unknown mechanisms. Transcriptomes of laser-dissected AIs, ovule cells, and ovaries from apomicts and AI-deficient mutants were analyzed to understand the pathways involved. The steps leading to AI mitosis and sexual pathway termination were determined using antibodies against arabinogalactan protein epitopes found to mark both sexual and aposporous female gametophyte lineages at inception. At most, four AIs differentiated near developing megaspores. The first expanding AI cell to contact the FM formed a functional AI that underwent mitosis soon after megaspore degeneration. Transcriptome analyses indicated that the enlarged, laser-captured AIs were arrested in the S/G2 phase of the cell cycle and were metabolically active. Further comparisons with AI-deficient mutants showed that AIs were enriched in transcripts encoding homologs of genes involved in, and potentially antagonistic to, known FM specification pathways. We propose that AI and FM cell contact provides cues required for AI mitosis and megaspore degeneration. Specific candidates to further interrogate AI-FM interactions were identified here and include arabinogalactan protein family genes.

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Section: Ai and Fm Cell Contact Precedes Megaspore Demise And Mitoticmentioning

confidence: 71%

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confidence: 99%

Asexual Female Gametogenesis Involves Contact with a Sexually-Fated Megaspore in Apomictic Hieracium

et al. 2018

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“…However, in the leaves that had been subjected to a temperature of 40 °C, an increase in the intensity of fluorescence signal was observed in the walls of phloem cells compared to the leaves that were growing at a low temperature (Figure 2G–I). JIM13 was found at the same locations as the JIM8 epitope (Figure A2A–F) and was additionally detected in the intercellular compartments (under intercellular compartments we define the localisation of epitope within the cytoplasm endomembrane system or organelles that are associated with the biosynthesis and secretion pathway to the wall, however these are not visible on the light microscope level [24]) of the prickles (Figure A2G–I). There were no changes in the distribution of the JIM13 epitope or in the intensity of the fluorescence signal between the analysed temperatures.…”

Section: Resultsmentioning

confidence: 99%

Hydroxyproline-Rich Glycoproteins as Markers of Temperature Stress in the Leaves of Brachypodium distachyon

Piński

Betekhtin

Sala

et al. 2019

IJMS

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Plants frequently encounter diverse abiotic stresses, one of which is environmental thermal stress. To cope with these stresses, plants have developed a range of mechanisms, including altering the cell wall architecture, which is facilitated by the arabinogalactan proteins (AGP) and extensins (EXT). In order to characterise the localisation of the epitopes of the AGP and EXT, which are induced by the stress connected with a low (4 °C) or a high (40 °C) temperature, in the leaves of Brachypodium distachyon, we performed immunohistochemical analyses using the antibodies that bind to selected AGP (JIM8, JIM13, JIM16, LM2 and MAC207), pectin/AGP (LM6) as well as EXT (JIM11, JIM12 and JIM20). The analyses of the epitopes of the AGP indicated their presence in the phloem and in the inner bundle sheath (JIM8, JIM13, JIM16 and LM2). The JIM16 epitope was less abundant in the leaves from the low or high temperature compared to the control leaves. The LM2 epitope was more abundant in the leaves that had been subjected to the high temperatures. In the case of JIM13 and MAC207, no changes were observed at the different temperatures. The epitopes of the EXT were primarily observed in the mesophyll and xylem cells of the major vascular bundle (JIM11, JIM12 and JIM20) and no correlation was observed between the presence of the epitopes and the temperature stress. We also analysed changes in the level of transcript accumulation of some of the genes encoding EXT, EXT-like receptor kinases and AGP in the response to the temperature stress. In both cases, although we observed the upregulation of the genes encoding AGP in stressed plants, the changes were more pronounced at the high temperature. Similar changes were observed in the expression profiles of the EXT and EXT-like receptor kinase genes. Our findings may be relevant for genetic engineering of plants with increased resistance to the temperature stress.

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“…Then, the cell walls are broken down, and lysigenous cavities filled with mucilage are formed. In a previous paper about the immunocytochemistry of Asteraceae ovules [ 36 ], arabinogalactan proteins (AGPs), hemicelluloses, and pectic epitopes in the transmitting tissue of Taraxacum ovules were analyzed; however, the mucilage cells were not analyzed.…”

Section: Introductionmentioning

confidence: 99%

Immunodetection of Pectic Epitopes, Arabinogalactan Proteins, and Extensins in Mucilage Cells from the Ovules of Pilosella officinarum Vaill. and Taraxacum officinale Agg. (Asteraceae)

Płachno

Kapusta

Świątek

et al. 2020

IJMS

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The main aim of this study was to compare the cytological difference between ovular mucilage cells in two Asteraceae species—Pilosella officinarum and Taraxacum officinale—in order to determine whether pectic epitopes, arabinogalactan proteins, or extensins are present. The immunocytochemical technique was used. Both the Taracacum and Pilosella genera have been used recently as models for understanding the mechanisms of apomixis. Knowledge of the presence of signal molecules (pectic epitopes, arabinogalactan proteins, and extensins) can help better understand the developmental processes in these plants during seed growth. The results showed that in Pilosella officinarum, there was an accumulation of pectins in the mucilage, including both weakly and highly esterified pectins, which was in contrast to the mucilage of Taraxacum officinale, which had low amounts of these pectins. However, Taraxacum protoplasts of mucilage cells were rich in weakly methyl-esterified pectins. While the mucilage contained arabinogalactan proteins in both of the studied species, the types of arabinogalactan proteins were different. In both of the studied species, extensins were recorded in the transmitting tissues. Arabinogalactan proteins as well as weakly and highly esterified pectins and extensins occurred in close proximity to calcium oxalate crystals in both Taraxacum and Pilosella cells.

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Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae)

Cited by 15 publications

References 54 publications

Asexual Female Gametogenesis Involves Contact with a Sexually-Fated Megaspore in Apomictic Hieracium

Asexual Female Gametogenesis Involves Contact with a Sexually-Fated Megaspore in Apomictic Hieracium

Hydroxyproline-Rich Glycoproteins as Markers of Temperature Stress in the Leaves of Brachypodium distachyon

Immunodetection of Pectic Epitopes, Arabinogalactan Proteins, and Extensins in Mucilage Cells from the Ovules of Pilosella officinarum Vaill. and Taraxacum officinale Agg. (Asteraceae)

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