Immunodetection of inactivated Francisella tularensis bacteria by using a quartz crystal microbalance with dissipation monitoring

Kleo, Karsten; Schäfer, Daniel; Klar, Stefanie; Jacob, Daniela; Grunow, Roland; Lisdat, Fred

doi:10.1007/s00216-012-6172-7

Cited by 25 publications

(13 citation statements)

References 42 publications

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“…The binding of the probe on the surface of the AuNPs with the genomic DNA will result in red colour while the absence of the target protein will result in blue or purple colour [65]. In another system, an antibody-based sensor using AuNPs was also developed for the detection of Francisella tularensis [66].…”

Section: Detection Of Whole Cell Bacteria or Virusmentioning

confidence: 99%

Gold Nanoparticles in Biosensing Analyses

Gopinath

Citartan

Lakshmipriya

et al. 2014

Nanoparticles' Promises and Risks

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Monitoring biomolecular interactions has become a constituent element of various medical and clinical applications. The importance of measuring the biomolecular interaction is evident as a potpourri of sensing strategies is currently and continuously devised. One of the strategies that can immensely enhance the sensitivity of the interaction is the one mediated by nanoparticles. Among the various nanoparticles, gold nanoparticle (AuNP) is opted for due to its capacity to improve the sensitivity and selectivity of biomolecular interactions. In addition to this, AuNP is easy to be manufactured, chemically inert, have uniform dispersibility and are able to react with chemically modified biomolecules. In this overview, the roles adopted by AuNP in various biosensing applications are discussed.

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Section: Detection Of Whole Cell Bacteria or Virusmentioning

confidence: 99%

Gold Nanoparticles in Biosensing Analyses

Gopinath

Citartan

Lakshmipriya

et al. 2014

Nanoparticles' Promises and Risks

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show abstract

“…Afterward, 10 μg/ml suspension of antigen (BSA) was added, and the solution was further incubated for 30 min under rotation. Then the solution was made up to 1ml with PBS buffer and centrifuged at 12000 rpm at 4°C for 1 hour, and was fi nally resuspended in sodium phosphate buffer (Kleo et al, 2012).…”

Section: Coupling Of Antigen (Bsa) With Aunpmentioning

confidence: 99%

Highly sensitive gold nanoparticle based electrochemical biosensor for detection of Antigen antibody interactions

Rawat¹,

Singh²,

Vasmatkar³

et al. 2017

Biosci. Biotech. Res. Comm

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The electrochemical biosensor was designed for label-free detection of bovine serum albumin (BSA). In the developed electrochemical sensor gold coated polycarbonate membrane of different 30, 50 and 100 nm pore sizes. were used for detection. The gold coated polycarbonate membranes were thiolated by 16-Mercaptohexadecanoic acid (MHDA) and then activated by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) for the binding of the antibody (BSA). After activation of the membranes, the BSA antibody was immobilized and the membranes were subjected to the detection of BSA antigen with the help of a homemade electrochemical setup. The specifi city of the antibody was cross-checked with a non-corresponding Prostate Specifi c Antigen (PSA). The specifi city and sensitivity of the designed biosensor along with the signal amplifi cation due to binding of antigen-gold nanoparticle conjugate was also determined. The impedance corresponding to each step of membrane modifi cation was observed and it was found that there was a sharp increase in the measured impedance from modifi cation to detection. Also, there was multiple fold increase in signal due to tagging of GNP. The impedance corresponding to different pore sized membranes was used to fi nd a suitable pored membrane for the sensitive detection and it was observed that 30 nm pore size membrane showed comparatively better result than 50 and 100 nm pore size membrane.

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“…Beside these optical methods gold nanoparticles (Au-NPs) can be applied efficiently for the mass sensitive detection of bacteria and viruses via quartz crystal microbalance (QCM) [27,28]. Furthermore nanocrystalline materials can be applied advantageously for sensorial bioelectrochemistry as antibody conjugates in front of the gate of field-effect transistors [29], for amperometric detection of enzyme produced hydrogen peroxide [30], for signal amplification in impedance spectroscopy [31] or for direct electron transfer within protein electrode structures [32,33].…”

Section: Introductionmentioning

confidence: 99%