Immunodetection Approaches and High-Performance Immunoaffinity Chromatography for an Analogue of Bovine Growth Hormone Releasing Factor at Trace Levels

Abstract. The combination of immunoassays with separation techniques such as chromatography and electrophoresis can provide both selectivity and sensitivity that is competitive with any method currently available for molecular analysis. Immunoassays can be carried out on-line and off-line, pre and post separation. The on-line post separation mode is the most promising for routine analysis because of the high throughput that can be achieved but also provides the greatest challenge with regard to compatibility of the interfaced systems. This paper reviews the various approaches that have been researched from a practical immunochemical point of view with emphasis on the special problems incurred with matrix compatibility for on-line post separation systems.

show abstract

Combining immunoassays with chromatographic and electrophoretic separation techniques ? a review

Shahdeo

Karnes

1998

Mikrochim Acta

View full text Add to dashboard Cite

show abstract

Capillary isoelectric focusing and affinity capillary electrophoresis approaches for the determination of binding constants for antibodies to the prion protein

Zhou

Wang

et al. 2004

Journal of Chromatography A

View full text Add to dashboard Cite

For the development of specific immunological assays, the binding of a specific antibody (Ab) to the target antigen (Ag) has to be relatively strong. In this study, we have utilized affinity capillary electrophoresis (ACE), a form of capillary zone electrophoresis (CZE) to determine the binding constant (Kb) of specific Abs against bovine serum albumin (BSA) and the healthy prion protein (PrPc), in buffer solutions at fixed pHs, approximating in vivo conditions. We have also utilized capillary isoelectric focusing (cIEF) to determine the complexity and recognition of the various isoforms of PrPc Abs towards their Ag, PrPc. Only ACE and CZE have been used to derive Kb values. The selected Abs for the prion protein can recognize both healthy and diseased states of the protein and are commercially available. The Kb values of PrPc Abs appear to be as strong as the anti-BSA (Ab to BSA) and other reported Kb values for proteins of similar size to PrPc. This appears to be one of the few reports on Kb values for any PrPc Abs, and their applications for in vitro immunoassays (e.g., enzyme-linked immunosorbent assays (ELISAs)). Such assays are being used to detect the infectious agent, PrPres, in brain and related matter/tissues.

show abstract

Derivatization of peptides and small proteins for improved identification and detection in capillary zone electrophoresis (CZE)

Liu

Cho

Strong

et al. 1999

Analytica Chimica Acta

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Immunodetection Approaches and High-Performance Immunoaffinity Chromatography for an Analogue of Bovine Growth Hormone Releasing Factor at Trace Levels

Cited by 9 publications

References 40 publications

Combining immunoassays with chromatographic and electrophoretic separation techniques ? a review

Combining immunoassays with chromatographic and electrophoretic separation techniques ? a review

Capillary isoelectric focusing and affinity capillary electrophoresis approaches for the determination of binding constants for antibodies to the prion protein

Derivatization of peptides and small proteins for improved identification and detection in capillary zone electrophoresis (CZE)

Contact Info

Product

Resources

About