Immunodeficient mouse models in the characterization of the protective immunity to influenza virus

Bot, Adrian; Isobe, H; Bona, Constantin A.

doi:10.1007/bf02820795

Cited by 2 publications

(1 citation statement)

References 7 publications

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“…Although IAV infection has been characterized in SCID mice as an absence of cell mediated and humoral immune responses post-vaccination [20] and prolonged weight loss with failure to clear virus [21], mice are not a natural host for IAV. Furthermore, laboratory-adapted viral strains used in most of these studies may not be relevant to fully virulent circulating strains.…”

Section: Introductionmentioning

confidence: 99%

Pigs with Severe Combined Immunodeficiency Are Impaired in Controlling Influenza A Virus Infection

Rajão

Loving²,

Waide³

et al. 2016

J Innate Immun

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Influenza A viruses (IAV) infect many host species, including humans and pigs. Severe combined immunodeficiency (SCID) is a condition characterized by a deficiency of T, B, and/or natural killer (NK) cells. Animal models of SCID have great value for biomedical research. Here, we evaluated the pathogenesis and the innate immune response to the 2009 H1N1 pandemic IAV (H1N1pdm09) using a recently identified line of naturally occurring SCID pigs deficient in T and B lymphocytes that still have functional NK cells. SCID pigs challenged with H1N1pdm09 showed milder lung pathology compared to the non-SCID heterozygous carrier pigs. Viral titers in the lungs and nasal swabs of challenged SCID pigs were significantly higher than in carrier pigs 7 days postinfection, despite higher levels of IL-1β and IFN-α in the lungs of SCID pigs. The lower levels of pulmonary pathology were associated with the T and B cell absence in response to infection. The higher viral titers, prolonged shedding, and delayed viral clearance indicated that innate immunity was insufficient for controlling IAV in pigs. This recently identified line of SCID pigs provides a valuable model to understand the immune mechanisms associated with influenza protection and recovery in a natural host.

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Section: Introductionmentioning

confidence: 99%

Pigs with Severe Combined Immunodeficiency Are Impaired in Controlling Influenza A Virus Infection

Rajão

Loving²,

Waide³

et al. 2016

J Innate Immun

View full text Add to dashboard Cite

show abstract

DNA and protein-generated chimeric molecules for delivery of influenza viral epitopes in mouse and humanized NSG transfer models

Mihaylova,

Manoylov,

Nikolova

et al. 2024

Human Vaccines & Immunotherapeutics

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Purified subunit viral antigens are weakly immunogenic and stimulate only the antibody but not the T cell-mediated immune response. An alternative approach to inducing protective immunity with small viral peptides may be the targeting of viral epitopes to immunocompetent cells by DNA and protein-engineered vaccines. This review will focus on DNA and protein-generated chimeric molecules carrying engineered fragments specific for activating cell surface co-receptors for inducing protective antiviral immunity. Adjuvanted protein-based vaccine or DNA constructs encoding simultaneously T - and B-cell peptide epitopes from influenza viral hemagglutinin, and scFvs specific for costimulatory immune cell receptors may induce a significant increase of anti-influenza antibody levels and strong CTL activity against virus-infected cells in a manner that mimics the natural infection. Here we summarize the development of several DNA and protein chimeric constructs carrying influenza virus HA317–41 fragment. The generated engineered molecules were used for immunization in intact murine and experimentally humanized NSG mouse models.

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Immunodeficient mouse models in the characterization of the protective immunity to influenza virus

Cited by 2 publications

References 7 publications

Pigs with Severe Combined Immunodeficiency Are Impaired in Controlling Influenza A Virus Infection

Pigs with Severe Combined Immunodeficiency Are Impaired in Controlling Influenza A Virus Infection

DNA and protein-generated chimeric molecules for delivery of influenza viral epitopes in mouse and humanized NSG transfer models

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