“…The following staining methods are used: Masson’s trichrome, to quantify connective and smooth muscle tissue; Weigert resorcin fucsin with previous oxidation, to observe elastic system fibers; and picrosirius red with polarization for observation of different collagen types. The main used immuno-histochemistry technique is avidin-biotin to identify collagenous proteins, elastin, and glycoproteins ( 8 ) and tubulin (Tubulin, beta III, Mouse Monoclonal Antibody) for nerves analysis.…”