Immunocytochemistry of Angiosarcomas: A Study of 19 Cases with Special Emphasis on the Applicability of Endothelial Cell Specific Markers to Routinely Prepared Tissues

Alles, Jens Uwe; Bosslet, Klaus

doi:10.1093/ajcp/89.4.463

Cited by 59 publications

(21 citation statements)

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“…Antibodies to FVIII-RA were the first to be used in this field. Focal and weak anti-FVIII-RA positivity can be detected only in a minority of angiosarcomas [2,5,20,29,39]. Our results show that this antibody stains mainly the well differentiated vascular areas of the angiosarcomas, while less differentiated areas consistently fail to reveal any positivity.…”

Section: Discussionmentioning

confidence: 53%

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Different immunoreactivity of endothelial markers in well and poorly differentiated areas of angiosarcomas

Poblet

González-Palacios

Jiménez

1996

Vichows Archiv A Pathol Anat

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This study evaluated the immunohistochemical staining of four endothelial cell markers in well differentiated and poorly differentiated areas of angiosarcomas. Formaldehyde-fixed, paraffin-embedded sections from eight angiosarcomas were studied using the antibodies anti-factor VIII-related antigen (FVIII-RA), Ulex europaeus I agglutinin, anti-CD34 (QBEND/10) and anti-CD31 (JC70). The immunostaining of the angiomatous (well differentiated) and solid (poorly differentiated) areas was separately analysed and specificity was evaluated in 20 non-vascular tumours. The antibody anti-CD31 and Ulex europaeus were the most sensitive markers staining well differentiated vasoformative structures and poorly differentiated solid areas. Anti-FVIII-RA and anti-CD34 did not stain undifferentiated malignant endothelial cells from solid areas. Ulex europueus and anti-CD34 showed very low specificity; in contrast, none of the non-vascular tumours expressed CD31 or FVIII-RA. JC70 (anti-CD31) appears to be the most useful marker in elucidating the vascular nature of angiosarcomas. Is important to emphasize the lack of specificity of Ulex europaeus and the low sensitivity of anti-CD34 and anti-FVIII-RA for poorly differentiated lesions.

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Section: Discussionmentioning

confidence: 53%

“…Endothelial cell labelling by these antibodies has been observed in cultured endothelial cells [12,13] and endothelial cells of non-neoplastic and benign endothelial lesions. However, the immunostaining of angiosarcomas with FVIII-RA is often disappointing [2,5,26,36,42].…”

Section: Introductionmentioning

confidence: 99%

Different immunoreactivity of endothelial markers in well and poorly differentiated areas of angiosarcomas

Poblet

González-Palacios

Jiménez

1996

Vichows Archiv A Pathol Anat

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“…VWF (factor VIII-related antigen), synthesized by endothelial cells and megakaryocvytes is a glycoprotein, that mediates platelet adhesion and stabilizes factor VIII at sites of vascular injury [38]. Moreover vWF can also be detected in thrombocytes.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemial study on the expression of von Willebrand factor (vWF) after onlay autogenous iliac grafts for lateral alveolar ridge augmentation

et al. 2013

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IntroductionThe main problems of autogenous bone transplants are their unpredictable atrophy and their loss of structure. One key factor lies in the poor revascularization of simple onlay grafts. The the aim of this study was to evaluate the revascularization processes in autogenous bone grafts from the iliac crest to the alveolar ridge.MethodsIn a sheep model, autogenous bone grafts were harvested from the iliac crest. A combination of a resorbable collagen membrane (CM) and deproteinized bovine bone material (DBBM) was used to modify the bone graft (experiment 2). This was compared with a simple onlay bone graft (control group, experiment 1). The amount of vessels in bone and connective tissue (CT), and the amount of CT were analyzed. The expression of von Willebrand factor (vWF) was compared between the two experimental groups using immunohistochemical analysis.ResultsThe ratio of the amount of vessels in bone and CT changed over time, and more vessels could be detected in bone at 12–16 weeks of graft healing. The number of vessels were significantly higher in experiment 2 than in experiment 1. More CT was found in experiment 1, whereas the amount of CT in both experiments decreased over time.ConclusionThis study shows a more intensive and extensive revascularization in experiment 2, as significantly more vessels were detected. The decreased amount of CT in experiment 2 clarifies its clinical superiority.

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“…Weibel-Palade bodies were present inconsistently, usually in the better differentiated neoplastic endothelial cells [ 35]. It is generally accepted that, in addition to the WPB, the immunoreactivity for factor VIII and CD31 constitutes one of the most speci® c ways to identify endothelial cells [ 33,36,37] . However, a failure to detect factor VIII or WPB does not exclude the presence of the endothelial cells, nor does it reliably correlate with the degree of tumor differentiation.…”

Section: Fig 5 Angiosarcoma Cells In the Intercapillary Stroma The mentioning

confidence: 99%

Primary Composite Angiogenic Leiomyosarcoma?Epithelioid Angiosarcoma of the Brain

Lach

Benoit

2000

Ultrastructural Pathology

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The authors describe a primary sarcoma of the brain with immunohistochemical and ultrastructural features of leiomyosarcoma as well as epithelioid hemangiosarcoma. The leiomyosarcomatous component consisted of spindle cells with well-developed external lamina, subsarcolemmal densities composed of micro® laments, pinocytic vesicles, and abundant intermediate ® laments, and showed immunohistochemical reactions for smooth muscle actin. The epithelioid part of the tumor contained scattered cells reactive for alkaline phosphatase as well as CD31 and factor VIII. Many epithelioid cells were lipidized and remarkably similar to``stromal cells'' of a hemangioblastoma. Occasional Weibel-Palade bodies, indicating endothelial differentiation, were present in scattered neoplastic cells. There were also cells with features intermediate between endothelium, pericytes and smooth muscle cells, and undifferentiated mesenchymal cells. The brain at the periphery of sarcoma showed conglomerates of well-differentiated capillaries, telangiectasias and small dysplastic arteries, features that raise the possibility of origin of this tumor from a preexisting vascular developmental abnormality.

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Immunocytochemistry of Angiosarcomas: A Study of 19 Cases with Special Emphasis on the Applicability of Endothelial Cell Specific Markers to Routinely Prepared Tissues

Cited by 59 publications

References 0 publications

Different immunoreactivity of endothelial markers in well and poorly differentiated areas of angiosarcomas

Different immunoreactivity of endothelial markers in well and poorly differentiated areas of angiosarcomas

Immunohistochemial study on the expression of von Willebrand factor (vWF) after onlay autogenous iliac grafts for lateral alveolar ridge augmentation

Primary Composite Angiogenic Leiomyosarcoma?Epithelioid Angiosarcoma of the Brain

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