Immunocytochemical visualization of coated pits and vesicles in human fibroblasts: Relation to low density lipoprotein receptor distribution

Anderson, Richard G. W.; Vasile, Eliza; Mello, Robert J.; Brown, Michael S.; Goldstein, Joseph L.

doi:10.1016/0092-8674(78)90276-3

Cited by 176 publications

(97 citation statements)

References 26 publications

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“…The GFP-NK␣ 1 signal was detected along the plasma membrane and as punctate structures within the cytosol ( Figure 1A). Analysis of GFP-NK␣ 1 revealed two distinct structures of 0.7-0.9-m diameter, mostly concentrated in the perinuclear region and vesicles of 0.2-0.3-m diameter throughout the cytoplasm and near the plasma membrane (consistent with the size of individual clathrin vesicles; Anderson et al, 1978;Gaidarov et al, 1999). Total cell Na ϩ ,K ϩ -ATPase activity was not different in A549 cells expressing stably the GFP-NK␣ 1 or the nontagged isoform ( Figure 1B).…”

Section: Incorporation Of Na ؉ K ؉ -Atpase-containing Vesicles Into mentioning

confidence: 83%

Analysis of Na⁺,K⁺-ATPase Motion and Incorporation into the Plasma Membrane in Response to G Protein–coupled Receptor Signals in Living Cells

Bertorello

Komarova

Smith

et al. 2003

MBoC

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Dopamine (DA) increases Na+,K+-ATPase activity in lung alveolar epithelial cells. This effect is associated with an increase in Na+,K+-ATPase molecules within the plasma membrane ( Ridge et al., 2002 ). Analysis of Na+,K+-ATPase motion was performed in real-time in alveolar cells stably expressing Na+,K+-ATPase molecules carrying a fluorescent tag (green fluorescent protein) in the α-subunit. The data demonstrate a distinct (random walk) pattern of basal movement of Na+,K+-ATPase–containing vesicles in nontreated cells. DA increased the directional movement (by 3.5 fold) of the vesicles and an increase in their velocity (by 25%) that consequently promoted the incorporation of vesicles into the plasma membrane. The movement of Na+,K+-ATPase–containing vesicles and incorporation into the plasma membrane were microtubule dependent, and disruption of this network perturbed vesicle motion toward the plasma membrane and prevented the increase in the Na+,K+-ATPase activity induced by DA. Thus, recruitment of new Na+,K+-ATPase molecules into the plasma membrane appears to be a major mechanism by which dopamine increases total cell Na+,K+-ATPase activity.

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Section: Incorporation Of Na ؉ K ؉ -Atpase-containing Vesicles Into mentioning

confidence: 83%

Analysis of Na⁺,K⁺-ATPase Motion and Incorporation into the Plasma Membrane in Response to G Protein–coupled Receptor Signals in Living Cells

Bertorello

Komarova

Smith

et al. 2003

MBoC

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“…This fibroblast cell line was used as a model for LDL receptor-expressing cells, since the receptor expression is well defined in this cell line (Anderson et al, 1978) and not well defined in the retinoblastoma cells. Results from experiments with retinoblastoma cells were similar ( Figures 5 and 6).…”

Section: Resultsmentioning

confidence: 99%

“…In this study, we report conditions to bind LDL molecules covalently to a second-generation photosensitizer, chlorin e6 (Ce6). Recognition by the LDL-receptor and uptake were tested in proliferating human fibroblasts with characteristically high receptor activity (Anderson et al, 1978). Subsequently, Ce6-LDL conjugates were used for the targeted photosensitization of human retinoblastoma cells in vitro.…”

mentioning

confidence: 99%

Photodynamic targeting of human retinoblastoma cells using covalent low-density lipoprotein conjugates

Schmidt-Erfurth

Diddens²,

Birngruber³

et al. 1997

Br J Cancer

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“…However, rather than depolymerize, actin polymerizes as coated vesicles move away from the membrane (229). In an early study of the distribution of clathrin coats on membranes of primary human fibroblasts, Anderson et al (6) observed that clathrin-coated pits that contained lowdensity lipoprotein (LDL) receptors lined up over stress fibers. A more recent study by light microscopy of GFPclathrin in live cells confirms that clathrin coats appear to organize in relation to cortical actin (302).…”

Section: A Ptdins(45)p 2 At the Plasma Membranementioning

confidence: 99%

Phosphoinositides in Constitutive Membrane Traffic

Roth

2004

Physiological Reviews

263

267

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Proteins that make, consume, and bind to phosphoinositides are important for constitutive membrane traffic. Different phosphoinositides are concentrated in different parts of the central vacuolar pathway, with phosphatidylinositol 4-phosphate predominate on Golgi, phosphatidylinositol 4,5-bisphosphate predominate at the plasma membrane, phosphatidylinositol 3-phosphate the major phosphoinositide on early endosomes, and phosphatidylinositol 3,5-bisphosphate found on late endocytic organelles. This spatial segregation may be the mechanism by which the direction of membrane traffic is controlled. Phosphoinositides increase the affinity of membranes for peripheral membrane proteins that function for sorting protein cargo or for the docking and fusion of transport vesicles. This implies that constitutive membrane traffic may be regulated by the mechanisms that control the activity of the enzymes that produce and consume phosphoinositides. Although the lipid kinases and phosphatases that function in constitutive membrane traffic are beginning to be identified, their regulation is poorly understood.

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Immunocytochemical visualization of coated pits and vesicles in human fibroblasts: Relation to low density lipoprotein receptor distribution

Cited by 176 publications

References 26 publications

Analysis of Na⁺,K⁺-ATPase Motion and Incorporation into the Plasma Membrane in Response to G Protein–coupled Receptor Signals in Living Cells

Analysis of Na⁺,K⁺-ATPase Motion and Incorporation into the Plasma Membrane in Response to G Protein–coupled Receptor Signals in Living Cells

Photodynamic targeting of human retinoblastoma cells using covalent low-density lipoprotein conjugates

Phosphoinositides in Constitutive Membrane Traffic

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Immunocytochemical visualization of coated pits and vesicles in human fibroblasts: Relation to low density lipoprotein receptor distribution

Cited by 176 publications

References 26 publications

Analysis of Na+,K+-ATPase Motion and Incorporation into the Plasma Membrane in Response to G Protein–coupled Receptor Signals in Living Cells

Analysis of Na+,K+-ATPase Motion and Incorporation into the Plasma Membrane in Response to G Protein–coupled Receptor Signals in Living Cells

Photodynamic targeting of human retinoblastoma cells using covalent low-density lipoprotein conjugates

Phosphoinositides in Constitutive Membrane Traffic

Contact Info

Product

Resources

About

Analysis of Na⁺,K⁺-ATPase Motion and Incorporation into the Plasma Membrane in Response to G Protein–coupled Receptor Signals in Living Cells

Analysis of Na⁺,K⁺-ATPase Motion and Incorporation into the Plasma Membrane in Response to G Protein–coupled Receptor Signals in Living Cells