Immunocytochemical typification of mesothelial cells in effusions: In vivo and in vitro models

Ferrández-Izquierdo, Antonio; Navarro-Fos, Samuel; González-Devesa, M; Gil-Benso, Rosario; Llombart‐Bosch, Antonio

doi:10.1002/dc.2840100313

Cited by 20 publications

(12 citation statements)

References 21 publications

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“…Intermediate filaments are important indicators of epithelial differentiation (13). Mesothelial cells are unique as although they are derived from mesoderm and express the mesenchymal intermediate filaments vimentin and desmin, they also express cytokeratins which are intermediate filaments characteristic of epithelial cells (14). While cytokeratins predominate in situ , cultured mesothelial cells were found to express small amount of cytokeratins (22).…”

Section: Discussionmentioning

confidence: 99%

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Mesothelial cells can detach from the mesentery and differentiate into macrophage-like cells

Katz

Balogh

Kiss

2011

Apmis

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Peritoneal cell suspension is composed of heterogeneous cell population. Macrophages are the most numerous cells among them. They can originate from different sources and can be resident, exudate and elicited. When we used Freund's adjuvant to elicit peritoneal macrophages, cells having large amount of caveolae on their plasma membrane appeared in the peritoneal wash. The number of these caveolae-rich cells increased by the time of the Freund's adjuvant treatment. Although their morphology was different form from the common macrophages, they were labelled with pan-macrophage antibodies. As the origin of these cells is unknown in this work, we tried to find out where they can originate from. Our interest turned towards the mesothelial cells. We found that the adjuvant treatment resulted in significant morphological changes in these cells and stimulate them to leave the surface of the mesentery. By the time of the adjuvant treatment, the macrophage markers expression increased in the mesothelial cells and more cells were found to detach from the mesentery. These results strongly suggest that under special stimuli mesothelial cells can leave the mesentery and differentiate into phagocytotic (macrophage-like) cells. These data raises the idea that mesothelial cells might not entirely differentiated and represent a multipotential cell lineage. To study whether this is the case we used anti-nestin antibody, which is a specific marker for multifunctional, multi-lineage progenitor cells. Mesothelial cells showed strong labelling with this antibody indicating that these cells really represent a 'young', not entirely differentiated cell population.

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Section: Discussionmentioning

confidence: 99%

“…Intermediate filaments are important indicators of epithelial differentiation (13). Rat mesothelial cells co‐express vimentin (mesenchymal) and the simple epithelial cytokeratins (14). While cytokeratins predominate in situ , the loss of cytokeratin production can be a sign of changing the phenotype.…”

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confidence: 99%

Mesothelial cells can detach from the mesentery and differentiate into macrophage-like cells

Katz

Balogh

Kiss

2011

Apmis

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“…Kawai et al 11 reported that RCA‐I is useful for differentiating between AC and RMC. However, Ferrandez‐Izquierdo et al 22 described that tissue should be treated with neuraminidase to identify cryptic PNA binding sites. Because RCA‐I is thought to bind to galactose and cryptic binding sites, 23 we considered that tissue should also be treated with neuraminidase to be able to better identify RCA‐I binding sites.…”

Section: Discussionmentioning

confidence: 99%

Comparative immunochemical study of lectin‐binding sites and cytoskeletal filaments in static and reactive mesothelium and adenocarcinoma

Ueda

Iwata²,

Takahashi

et al. 2001

Pathology International

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In cytological preparations, reactive mesothelial cells (RMC) in serous effusions are sometimes difficult to distinguish from adenocarcinoma cells (AC). RMC and AC can be distinguished by lectin-binding patterns, but the pattern of binding of lectins to normal mesothelium is not well defined. We investigated the expression of cytoskeletal filaments, cytokeratin (CK) and vimentin (VM), and the cell surface binding pattern of 10 lectins (HPA, SBA, ABA, DSA, PNA, RCA-I, UEA-I, LTA, WGA and ConA) in the serosa of 48 adenocarcinoma specimens. We also investigated the usefulness of six lectins (HPA, SBA, RCA-I, UEA-I, LTA and WGA) in identification of RMC and AC in 16 serous effusions. DSA reactivity was significantly higher (P < 0.05) in static mesothelial cells (SMC) than in RMC. Reactivity for LTA and ConA was significantly lower (P < 0.05) in SMC than in RMC. Anti-CK and anti-VM immunoreactivity was always positive in RMC and almost negative in SMC. In serous effusions, HPA, SBA and UEA-I binding was evident in 100, 88 and 81% of AC, respectively. Little to no binding of HPA, SBA or UEA-I was detected in RMC. Our results suggest that the morphological differences between SMC and RMC are likely to be due to differences in cytoskeletal composition, with accompanying changes in cell-surface lectin-binding patterns. HPA, SBA and UEA-I are likely to be useful markers for identification of RMC and AC in cytology.

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“…MOC-31 is a sensitive epithelial marker and is commonly used in a panel to detect adenocarcinoma in effusions [11,12,13]. To distinguish between benign and malignant mesothelial populations we included the muscle marker desmin, which has been reported as a marker for benign mesothelial cells and is generally negative in mesothelioma and adenocarcinoma [14,15,16]. …”

Section: Introductionmentioning

confidence: 99%

The Diagnostic Utility of D2-40, Calretinin, CK5/6, Desmin and MOC-31 in the Differentiation of Mesothelioma from Adenocarcinoma in Pleural Effusion Cytology

Hyun¹,

Barnes²,

Tabatabai³

2012

Acta Cytologica

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Objective: To evaluate the utility of the lymphatic endothelial marker D2-40, along with calretinin, CK5/6, desmin and MOC-31, in differentiating mesothelioma and adenocarcinoma in pleural effusion cytology. Study Design: Forty-five pleural effusion cases representing confirmed reactive effusions (13), mesotheliomas (11) and metastatic adenocarcinomas (21) were immunostained with antibodies against D2-40, calretinin, CK5/6, desmin and MOC-31. Results: D2-40 showed membranous staining in 82% of mesotheliomas and 77% of reactive effusions. Calretinin and CK5/6 were positive in 100 and 64% of mesotheliomas, and 92 and 31% of reactive effusions, respectively. All adenocarcinomas showed lack of staining with these markers. Desmin was negative in all malignant cases and positive in 85% of reactive effusions. All adenocarcinomas were positive for MOC-31 and negative for the remaining markers. Conclusion: Calretinin was the most sensitive in detecting mesothelial differentiation, followed by D2-40. Although useful, D2-40 necessitated cautious interpretation due to occasional focal/weak positivity, particularly in limited cellularity samples. The muscle marker desmin was useful in differentiating benign from malignant effusions but not in distinguishing mesotheliomas from adenocarcinomas. MOC-31 was both highly sensitive and specific for detecting adenocarcinoma and was useful as part of a panel of stains in differentiating cells of mesothelial origin from adenocarcinoma.

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Immunocytochemical typification of mesothelial cells in effusions: In vivo and in vitro models

Cited by 20 publications

References 21 publications

Mesothelial cells can detach from the mesentery and differentiate into macrophage-like cells

Mesothelial cells can detach from the mesentery and differentiate into macrophage-like cells

Comparative immunochemical study of lectin‐binding sites and cytoskeletal filaments in static and reactive mesothelium and adenocarcinoma

The Diagnostic Utility of D2-40, Calretinin, CK5/6, Desmin and MOC-31 in the Differentiation of Mesothelioma from Adenocarcinoma in Pleural Effusion Cytology

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