Immunocytochemical studies of the interactions of cadherins and catenins in the earlyXenopus embryo

Kurth, Thomas; Fesenko, Irina; Schneider, Stephan; Münchberg, Frank E.; Joos, Thomas; Spieker, Thomas P.; Hausen, Peter

doi:10.1002/(sici)1097-0177(199906)215:2<155::aid-dvdy8>3.0.co;2-s

Cited by 33 publications

(3 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To test a functional requirement for adherens junctions in orienting the spindle, we focused on maternal C-cadherin (cadherin 3), which is expressed at the highest level in Stage 10-11 Xenopus embryos (Heasman et al, 1994; Lee and Gumbiner, 1995). We used two constructs to manipulate C-cadherin in the tissue: C-cadherin FL-6xmyc (CdhFL: Full length C-cadherin with 6xmyc tags at the intracellular c-terminus) and C-cadherin ΔC - 6xmyc (CdhΔC: C-cadherin with extracellular and transmembrane domains, but lacking the cytosolic domain) (Figure 4A) (Kurth et al, 1999). CdhFL- and CdhΔC-injected embryos developed normally up to Stage 10/11 (Figure S3A), but the majority of embryos failed to complete gastrulation (Lee and Gumbiner, 1995) (and data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…Xenopus laevis embryos were obtained and injected as described previously (Woolner and Papalopulu, 2012). RNA was synthesised as described previously (Sokac et al, 2003) and microinjected at the following needle concentrations: 0.5 mg/ml GFP-α-tubulin; 0.1 mg/ml cherry-histone2B(Kanda et al, 1998); 0.125 mg/ml cadherin 3a full length:6x myc-tag; 0.125 mg/ml cadherin 3a deleted cytosolic domain:6x myc-tag (Kurth et al, 1999). For mosaic expression of β-catenin-GFP (Addgene plasmid #16839, Randall Moon) and GFP-LGN (sub-cloned into pCS2+ from Addgene plasmid #37360, Iain Cheeseman), RNA was injected into a single cell at the 4-cell stage at 0.25 mg/ml (needle concentration).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Decoupling the roles of cell shape and mechanical stress in orienting and cueing epithelia mitosis

Nestor-Bergmann

Stooke‐Vaughan

Goddard

et al. 2017

Preprint

View full text Add to dashboard Cite

19Distinct mechanisms involving cell shape and mechanical force are known to influence the 20 rate and orientation of division in cultured cells. However, uncoupling the impact of shape and 21 force in tissues remains challenging. Combining stretching of Xenopus laevis tissue with a 22 novel method of inferring relative mechanical stress, we find separate roles for cell shape in 23 orientating division and mechanical stress in cueing division. We demonstrate that division 24 orientation is best predicted by an axis of cell shape defined by the position of tricellular 25 junctions, which aligns exactly with the principal axis of local cell stress rather than the tissue-26 level stress. The alignment of division to cell shape requires functional cadherin, but is not 27 sensitive to relative cell stress magnitude. In contrast, cell proliferation rate is more directly 28 regulated by mechanical stress, being correlated with relative isotropic stress, and can be 29 decoupled from cell shape when myosin II is depleted.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Decoupling the roles of cell shape and mechanical stress in orienting and cueing epithelia mitosis

Nestor-Bergmann

Stooke‐Vaughan

Goddard

et al. 2017

Preprint

View full text Add to dashboard Cite

show abstract

“…The pCS2‐Oct‐3/4 was constructed by cloning the full‐length Oct‐3/4 cDNA into the ClaI site of pCS2. The other expression plasmids were pEVRF‐Oct‐3/4, Oct‐3/4‐ΔN, Oct‐3/4‐ΔC (Ben‐Shushan et al , 1998); pOct1 (Tanaka and Herr, 1990); pOct‐2 (Clerc et al , 1988); pOct‐6 (Meijer et al , 1992); Flag‐β‐catenin, Flag‐DP, Flag‐D32N, Flag‐S33, Flag‐Axin1, Myc‐Axin1 and GFP (Amit et al , 2002); pCS2‐xWnt‐8 (Kelly et al , 1995); pCS2‐xSiamois (Lemaire et al , 1995); pCS2‐x β‐catenin (Kurth et al , 1999) and TOP‐Flash and FOP‐Flash (Korinek et al , 1997). The pLKO.1 lentivral vector‐expressing shRNA against β‐catenin was bought from Openbiosystems (Cat RMM4534).…”

Section: Methodsmentioning

confidence: 99%

Oct-3/4 regulates stem cell identity and cell fate decisions by modulating Wnt/β-catenin signalling

Abu-Remaileh

Gerson

Farago

et al. 2010

EMBO J

View full text Add to dashboard Cite

Although the transcriptional regulatory events triggered by Oct-3/4 are well documented, understanding the proteomic networks that mediate the diverse functions of this POU domain homeobox protein remains a major challenge. Here, we present genetic and biochemical studies that suggest an unexpected novel strategy for Oct-3/4-dependent regulation of embryogenesis and cell lineage determination. Our data suggest that Oct-3/4 specifically interacts with nuclear b-catenin and facilitates its proteasomal degradation, resulting in the maintenance of an undifferentiated, early embryonic phenotype both in Xenopus embryos and embryonic stem (ES) cells. Our data also show that Oct-3/4-mediated control of b-catenin stability has an important function in regulating ES cell motility. Down-regulation of Oct-3/4 increases b-catenin protein levels, enhancing Wnt signalling and initiating invasive cellular activity characteristic of epithelialmesenchymal transition. Our data suggest a novel mode of regulation by which a delicate balance between b-catenin, Tcf3 and Oct-3/4 regulates maintenance of stem cell identity. Altering the balance between these proteins can direct cell fate decisions and differentiation.

show abstract

Establishment of mesodermal gene expression patterns in earlyXenopusembryos: The role of repression

Kurth

Meißner

Schäckel

et al. 2005

Developmental Dynamics

Self Cite

View full text Add to dashboard Cite

In Xenopus, activin-like signals are able to induce and pattern mesoderm in a concentration-dependent manner. Previous experiments demonstrated that discrete gene expression patterns can be formed in animal cap explants as a response to graded activin signals. We analyzed the spatiotemporal appearance of goosecoid (gsc), chordin (chd), and Xbrachyury (Xbra) mRNAs in whole Xenopus embryos ectopically expressing activin or BVg1. To discriminate between direct transcriptional regulation and indirect, protein synthesis-dependent effects of ectopic signals, we combined overexpression studies and cycloheximide treatment. Our experiments revealed long-range signaling of activin/BVg1, but the expression patterns of gsc, chd, and Xbra in response to activin/BVg1 indicated that repressors are essential to establish the proper expression of these genes. Analysis of endogenous gsc, chd, and Xbra transcript distribution in embryos treated with cycloheximide supported this concept. We, therefore, conclude that inhibition is fundamental during early embryonic patterning. Developmental Dynamics 233:418 -429, 2005.

show abstract

Immunocytochemical studies of the interactions of cadherins and catenins in the earlyXenopus embryo

Cited by 33 publications

References 77 publications

Decoupling the roles of cell shape and mechanical stress in orienting and cueing epithelia mitosis

Decoupling the roles of cell shape and mechanical stress in orienting and cueing epithelia mitosis

Oct-3/4 regulates stem cell identity and cell fate decisions by modulating Wnt/β-catenin signalling

Establishment of mesodermal gene expression patterns in earlyXenopusembryos: The role of repression

Contact Info

Product

Resources

About