Immunocytochemical staining for stratifin and <scp>OCIAD</scp>2 in bronchial washing specimens increases sensitivity for diagnosis of lung cancer

Itoguchi, Naoe; Nakagawa, Takumi; Murata, Yoshihiko; Li, D; Shiba-Ishii, Aya; Minami, Yuko; Noguchi, Masayuki

doi:10.1111/cyt.12220

Cited by 17 publications

(19 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, some studies found that SFN could facilitate progression and development of lung adenocarcinoma at an early stage [7]. Besides, combined with OCIAD2, immunocytochemical staining for SFN could also enhance diagnostic sensitivity for lung cancers [8]. For breast cancer, it is determined that the biology of SFN is important in cellular movement and is contingent on breast cancer subtype [9].…”

Section: Introductionmentioning

confidence: 99%

Expression profile and prognostic value of SFN in human ovarian cancer

Zeng

et al. 2019

Bioscience Reports

View full text Add to dashboard Cite

Ovarian cancer is a highly lethal cancer in females. Therefore, it is necessary to explore effective biomarkers for the diagnosis and prognosis of the disease. Stratifin (SFN) is a cell cycle checkpoint protein that has been reported to be involved in oncogenesis. Our studies detected the expression of SFN in ovarian cancer by Oncomine, Human Protein Atlas database and ULCAN database. Meanwhile, we found its coexpression gene by cBioPortal online tool and validated their expression in different ovarian cancer cells by western blot and reverse transcription quantitative PCR. Then, we also investigated their prognostic values via the Kaplan–Meier plotter database in different subtypes of ovarian cancer patients. The results demonstrated that SFN was found to be increased in ten various ovarian cancer datasets, compared with healthy tissues. Additionally, up-regulation of SFN expression is associated with age and cancer grades. The higher expression of SFN in all patients with ovarian cancers is significantly correlated with worse postprogression survival. In addition, high SFN expression is associated with significantly worse overall survival in patients who received chemotherapy contains gemcitabine, taxol, taxol+platin, paclitaxel and avastin. In human ovarian carcinoma SKOV3 and A2780 cells, the expression of SFN and its coexpression gene MICB were also increased at protein and mRNA levels compared with the normal ovarian epithelial cells. Based on above results, overexpression of SFN was correlated with the prognosis in ovarian cancer. The present study might be useful for better understanding the clinical significance of SFN mRNA.

show abstract

Section: Introductionmentioning

confidence: 99%

Expression profile and prognostic value of SFN in human ovarian cancer

Zeng

et al. 2019

Bioscience Reports

View full text Add to dashboard Cite

show abstract

“…We have extensively studied the gene expression profiles of early stage adenocarcinoma and found several genes that are overexpressed in early invasive tumors but not in adenocarcinoma in situ . These genes include stratifin (SFN) [9], immunoglobulin binding protein 1 (IGBP1), [10] ovarian carcinoma immunoreactive antigen domain 2 (OCIAD2), [11] and dimethylarginine dimethylaminohydrolase 2 (DDAH2) [12]. In the course of our investigations, we realized that overexpression of all these genes does not occur through genetic alteration but is associated with epigenetic abnormality.…”

Section: Introductionmentioning

confidence: 99%

DNA hypomethylation-related overexpression of SFN, GORASP2 and ZYG11A is a novel prognostic biomarker for early stage lung adenocarcinoma

et al. 2019

View full text Add to dashboard Cite

Although alteration of DNA methylation in advanced cancer has been extensively investigated, few data for early-stage lung adenocarcinoma are available. Here, we compared DNA methylation profiles between adenocarcinoma in situ (AIS) and early invasive adenocarcinoma using the Infinium methylation array to investigate methylation abnormalities causing early progression of adenocarcinomas. We focused on differentially methylated sites which were located in promoter CpG islands or shore regions, and identified 579 hypermethylated sites and 23 hypomethylated sites in early invasive adenocarcinoma relative to AIS and normal lung. These hypermethylated genes were significantly associated with neuronal pathways such as the GABA receptor and serotonin signaling pathways. Among the hypomethylated genes, we found that GORASP2, ZYG11A, and SFN had significantly lower methylation rates at the shore regions and significantly higher protein expression in invasive adenocarcinoma. Moreover, overexpression of those proteins was strongly associated with patient’s poor outcome. Despite DNA demethylation at the promoter region might be rare relative to DNA hypermethylation, we identified 2 new genes, GORASP2 and ZYG11A, which show hypomethylation and overexpression in invasive adenocarcinoma, suggesting that they have important functions in tumor cells. These genes may be clinically applicable as prognostic indicators and could be potential novel target molecules for drug development.

show abstract

“…OCIAD1 and OCIAD2 are human cancer-related proteins implicated in ovarian 1 , thyroid 2 and lung cancers 3 , and in various hematological neoplasms 4 , 5 including multiple myeloma 6 and neutrophilia 7 . Their names derive from the first report on OCIAD1 (Ovarian Carcinoma Immunoreactive Antigen domain-containing protein 1), which was found in ascites fluid of patients with metastatic ovarian cancer and mapped to chromosome 4p11 8 .…”

Section: Introductionmentioning

confidence: 99%

“…Although OCIAD2 expression varies among different cancers, its precise function remains unknown - while high levels of OCIAD2 are reported in ovarian mucinous tumors 1 and lung carcinomas 3 , 13 ; significantly reduced OCIAD2 expression is reported in liver and gastric carcinomas 14 , glioblastomas 15 and chronic lymphocytic leukemia 16 . Further, loss of OCIAD2 function promotes cancer progression by increasing activation of the PI3K/Akt pathway 17 .…”

Section: Introductionmentioning

confidence: 99%

A double helical motif in OCIAD2 is essential for its localization, interactions and STAT3 activation

Sinha

Bheemsetty

Inamdar

2018

Sci Rep

View full text Add to dashboard Cite

The Ovarian Carcinoma Immunoreactive Antigen domain (OCIAD) - containing proteins OCIAD1/Asrij and OCIAD2, are implicated in several cancers and neurodegenerative diseases. While Asrij has a conserved role in facilitating STAT3 activation for JAK/STAT signaling, the expression and function of OCIAD2 in non-cancerous contexts remains unknown. Here, we report that ociad2 neighbors ociad1/asrij in most vertebrate genomes, and the two genes likely arose by tandem gene duplication, probably somewhere between the Ordovician and Silurian eras. We show that ociad2 expression is higher in the mouse kidney, liver and brain relative to other tissues. OCIAD2 localizes to early endosomes and mitochondria, and interacts with Asrij and STAT3. Knockdown and overexpression studies showed that OCIAD2 is essential for STAT3 activation and cell migration, which could contribute to its role in tumor metastasis. Structure prediction programs, protein disruption studies, biochemical and functional assays revealed a double helical motif in the OCIA domain that is necessary and sufficient for its localization, interactions and STAT3 activation. Given the importance of JAK/STAT signaling in development and disease, our studies shed light on the evolution and conserved function of the OCIA domain in regulating this pathway and will be critical for understanding this clinically important protein family.

show abstract

Immunocytochemical staining for stratifin and OCIAD2 in bronchial washing specimens increases sensitivity for diagnosis of lung cancer

Cited by 17 publications

References 22 publications

Expression profile and prognostic value of SFN in human ovarian cancer

Expression profile and prognostic value of SFN in human ovarian cancer

DNA hypomethylation-related overexpression of SFN, GORASP2 and ZYG11A is a novel prognostic biomarker for early stage lung adenocarcinoma

A double helical motif in OCIAD2 is essential for its localization, interactions and STAT3 activation

Contact Info

Product

Resources

About