Immunocytochemical localization of the ?6 subunit of the ?-aminobutyric acidA receptor in the rat nervous system

Gutiérrez, Antonia; Khan, Z U; Blas, Angel L. De

doi:10.1002/(sici)1096-9861(19960212)365:3<504::aid-cne12>3.0.co;2-q

Cited by 50 publications

(22 citation statements)

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“…Light and electron microscopy immunocytochemistry have shown the almost exclusive presence of a6 subunit peptide in granule cells ( Fig. 3; Baude et al, 1992;Thompson et al, 1992;Gutiérrez et al, 1996). The a1-subunit peptide is also expressed by cerebellar granule cells (Baude et al, 1992;Gutiérrez et al, 1994) and al and a6 colocalize in some cerebellar synapses (Baude et al, 1992).…”

Section: Discussionmentioning

confidence: 92%

The α₁ and α₆ Subunits Can Coexist in the Same Cerebellar GABA_A Receptor Maintaining Their Individual Benzodiazepine‐Binding Specificities

Khan

Gutiérrez

Blas

1996

Journal of Neurochemistry

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Two GABAA receptor subunit‐specific antibodies anti‐α6 and anti‐α1 have been used for elucidating the relationship between the presence of α1 and/or α6 subunits in the cerebellar GABAA receptors and the benzodiazepine‐binding specificity. Receptor immunoprecipitation with the subunit‐specific antibodies shows that 39% of the cerebellar GABAA receptors have α6, whereas 76% of the receptors have α1 as determined by [3H]muscimol binding. Results show that 42–45% of the receptors having α6 also have α1, whereas 13–15% of the receptors that contain α1 also have α6. The immunoprecipitation results as well as immunopurification and immunoblotting experiments reveal the existence of three types of cerebellar GABAA receptors; i.e., one has both α1 and α6 subunits, a second type has α1 but not α6, and a third type has α6 but not α1 subunits. The results also show that receptors where α1 and α6 subunits coexist have two pharmacologically different benzodiazepine‐binding properties, each associated with a different α subunit. The α1 subunit contributes the high‐affinity binding of [3H]Ro 15‐1788 (flumazenil) and the diazepam‐sensitive binding of [3H]Ro 15‐4513. The α6 subunit contributes the diazepam‐insensitive binding of [3H]Ro 15‐4513, but it does not bind [3H]Ro 15‐1788 with high affinity. Thus, in the cerebellar α1–α6 GABAA receptors, there is no dominance of the pharmacology of one α subunit over the other.

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Section: Discussionmentioning

confidence: 92%

The α₁ and α₆ Subunits Can Coexist in the Same Cerebellar GABA_A Receptor Maintaining Their Individual Benzodiazepine‐Binding Specificities

Khan

Gutiérrez

Blas

1996

Journal of Neurochemistry

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“…Receptor cluster density increases slightly with distance from the soma. The total number of immunoreactive puncta varied substantially between the cells, but the average density of immunoreactive puncta (about 0.1 puncta/m dendritic length) Greferath et al (1995) 11 Greferath et al (1994b) 12 Greferath et al (1993) 13 Grigorenko & Yeh (1994) Gutiérrez et al (1996) 19 Hughes et al (1989) 20 Hughes et al (1991) 21 Khan et al (1996) 22 Koulen et al (1998a) 23 Koulen et al (1997) 24 Koulen et al (1996) 25 Lin et al (2000) 26 Macri et al (2000) 27 Mü ller et al (1992) 28 Pourcho & Owczarzak (1991) 29 Sassoè-Pognetto et al (1994) 30 Vardi & Sterling (1994) 31 Wä ssle et al (1998) stayed relatively constant for ON-and OFF-parasol and midget ganglion cells. Parasol and midget cells also did not differ in the distribution of receptor clusters containing the ␣1 subunit of the GABA A receptor.…”

Section: Localization Of Gaba a And Glycine Receptors On Midget And Pmentioning

confidence: 99%

Distribution of GABA and glycine receptors on bipolar and ganglion cells in the mammalian retina

Grünert

2000

Microsc. Res. Tech.

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“…27,28,[44][45][46][47][48][49][50][51] More recently, the differential distribution of many more subunits has been studied. 25,28,[52][53][54] Each subunit is expressed in characteristic strata in the IPL 25 (Fig. 1).…”

Section: Distribution Of Gabaa Receptor Subunitsmentioning

confidence: 99%

DISTRIBUTION OF GABA_A AND GLYCINE RECEPTORS IN THE MAMMALIAN RETINA

Grünert

1999

Clin Exp Pharma Physio

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1. GABA and glycine mediate synaptic inhibition via specific neurotransmitter receptors. Molecular cloning studies have shown that there is a great diversity of receptors for these two neurotransmitters. In the present paper, the distribution of GABAA and glycine receptors in the mammalian retina is reviewed. 2. In situ hybridization, immunocytochemistry with subunit-specific antibodies and single cell injection were used to analyse the localization of receptor subunits. Specific subunits are expressed in characteristic strata of the inner plexi-form layer, suggesting that different functional circuits involve specific subtypes of neurotransmitter receptors. 3. Different cell types express different combinations of receptor subunits and an individual neuron can express several receptor isoforms at distinct post-synaptic sites.

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Immunocytochemical localization of the ?6 subunit of the ?-aminobutyric acidA receptor in the rat nervous system

Cited by 50 publications

References 41 publications

The α₁ and α₆ Subunits Can Coexist in the Same Cerebellar GABA_A Receptor Maintaining Their Individual Benzodiazepine‐Binding Specificities

The α₁ and α₆ Subunits Can Coexist in the Same Cerebellar GABA_A Receptor Maintaining Their Individual Benzodiazepine‐Binding Specificities

Distribution of GABA and glycine receptors on bipolar and ganglion cells in the mammalian retina

DISTRIBUTION OF GABA_A AND GLYCINE RECEPTORS IN THE MAMMALIAN RETINA

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Immunocytochemical localization of the ?6 subunit of the ?-aminobutyric acidA receptor in the rat nervous system

Cited by 50 publications

References 41 publications

The α1 and α6 Subunits Can Coexist in the Same Cerebellar GABAA Receptor Maintaining Their Individual Benzodiazepine‐Binding Specificities

The α1 and α6 Subunits Can Coexist in the Same Cerebellar GABAA Receptor Maintaining Their Individual Benzodiazepine‐Binding Specificities

Distribution of GABA and glycine receptors on bipolar and ganglion cells in the mammalian retina

DISTRIBUTION OF GABAA AND GLYCINE RECEPTORS IN THE MAMMALIAN RETINA

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The α₁ and α₆ Subunits Can Coexist in the Same Cerebellar GABA_A Receptor Maintaining Their Individual Benzodiazepine‐Binding Specificities

The α₁ and α₆ Subunits Can Coexist in the Same Cerebellar GABA_A Receptor Maintaining Their Individual Benzodiazepine‐Binding Specificities

DISTRIBUTION OF GABA_A AND GLYCINE RECEPTORS IN THE MAMMALIAN RETINA