Immunocytochemical localization of RNA polymerase I in the fibrillar centers of nucleoli

Scheer, Ulrich; Raška, Ivan

doi:10.1007/978-94-010-9166-4_27

Cited by 37 publications

(11 citation statements)

References 11 publications

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“…The failure to detect DNA in the DFC, together with the observation that RNA polymerase I molecules were mainly located in the FCs (24,25), led these authors to conclude that the FC was the site of ribosomal transcription and not the DFC, as generally accepted (5,6), since, according to these authors, DNA and RNA polymerase I molecules were both present only in the FC. This conclusion must be revised in the light of our data demonstrating that DNA filaments are also distributed in a portion of the DFC.…”

Section: Discussionmentioning

confidence: 99%

Localization of DNA in the fibrillar components of the nucleolus: a cytochemical and morphometric study.

Derenzini¹,

Farabegoli²,

Trerè³

1993

J Histochem Cytochem.

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We studied the distribution of DNA in human circulating lymphocyte nucleoli using three different cytochemical methods for selective visualization of DNA in thin sections: the Feulgen-like osmium-ammine reaction, the NAMA-Ur procedure, and the osmium-ammine staining in glycine buffer, pH 1.5. All three methods indicated the presence of uniformly distributed, highly decondensed DNA filaments forming a large solitary agglomerate in the central part of the nucleolar area, corresponding to the solitary large fibrillar center (FC) as revealed by uranium and lead staining. We also studied the relationship between DNA agglomerates and nucleolar fibtillat components in resting and phytohemagglutinin (PIU)-stimulated lymphocytes by morphometric analysis of the areas occupied by these structures. In resting lymphocytes the mean area of the DNA agglomerates was 0.479 pm2 2 0.161 SD, whereas that of FCs was

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Section: Discussionmentioning

confidence: 99%

Localization of DNA in the fibrillar components of the nucleolus: a cytochemical and morphometric study.

Derenzini¹,

Farabegoli²,

Trerè³

1993

J Histochem Cytochem.

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“…The intensity of the ISH signal for the plasmids in NOY 558 under inhibition was significantly higher when compared with cells without inhibition (Figures 2E and 7E). As already discussed (Goessens, 1984;Scheer and Raska, 1987), DNA may be more accessible to the probe if not transcribed.…”

Section: Active Rdna Transcription Is Necessary For the Colocalizatiomentioning

confidence: 92%

Assembly and Functional Organization of the Nucleolus: Ultrastructural Analysis ofSaccharomyces cerevisiaeMutants

Trumtel

Léger-Silvestre

Gleizes

et al. 2000

MBoC

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Using Saccharomyces cerevisiae strains with genetically modified nucleoli, we show here that changing parameters as critical as the tandem organization of the ribosomal genes and the polymerase transcribing rDNA, although profoundly modifying the position and the shape of the nucleolus, only partially alter its functional subcompartmentation. High-resolution morphology achieved by cryofixation, together with ultrastructural localization of nucleolar proteins and rRNA, reveals that the nucleolar structure, arising upon transcription of rDNA from plasmids by RNA polymerase I, is still divided in functional subcompartments like the wild-type nucleolus. rRNA maturation is restricted to a fibrillar component, reminiscent of the dense fibrillar component in wild-type cells; a granular component is also present, whereas no fibrillar center can be distinguished, which directly links this latter substructure to rDNA chromosomal organization. Although morphologically different, the mininucleoli observed in cells transcribing rDNA with RNA polymerase II also contain a fibrillar subregion of analogous function, in addition to a dense core of unknown nature. Upon repression of rDNA transcription in this strain or in an RNA polymerase I thermosensitive mutant, the nucleolar structure falls apart (in a reversible manner), and nucleolar constituents partially relocate to the nucleoplasm, indicating that rRNA is a primary determinant for the assembly of the nucleolus.

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“…Data are more abundant for animal cells. [49,50], using immunoelectron microscopy, and Thiry [56], using in situ hybridization have suggested that in animal nucleoli the rDNA is located exclusively in the fibrillar centres. Recently Raska et al [46] have observed, with specific antiserum, that RNA polymerase I is localized mainly in the fibrillar centres but is also present in the DFC.…”

Section: What Is the Molecular Composition And Organization Of The Numentioning

confidence: 99%

The nucleolonema of plant and animal cells: a comparison

Deltour¹,

Motté²

1990

Biology of the Cell

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Summary -Depending on the author and the animal or plant origin of the material under study, the term "nucleolonema" is used in different contexts and thus indicates nucleolar ultrastructures that are different. In this paper, we attempt to clarify this state of affairs and to propose a definition for the plant cell nucleolonema nucleolonema / nucleolus / fibrillar centre / dense fibrillar component / rDNA

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Immunocytochemical localization of RNA polymerase I in the fibrillar centers of nucleoli

Cited by 37 publications

References 11 publications

Localization of DNA in the fibrillar components of the nucleolus: a cytochemical and morphometric study.

Localization of DNA in the fibrillar components of the nucleolus: a cytochemical and morphometric study.

Assembly and Functional Organization of the Nucleolus: Ultrastructural Analysis ofSaccharomyces cerevisiaeMutants

The nucleolonema of plant and animal cells: a comparison

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