Immunocytochemical Localization of Prolactin-Releasing Peptide in the Rat Brain

Maruyama, Minoru

doi:10.1210/en.140.5.2326

Cited by 82 publications

(53 citation statements)

References 7 publications

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“…They were then incubated with primary antibodies (listed in Table 1) in PBS containing 1% normal horse serum and 0.4% Triton X-100 (except that for the lipids detection) overnight at 4°C, followed by detection with biotinylated secondary antibodies and an avidin-biotin complex kit (Vector Laboratories, Burlingame, CA) [39]. A positive reaction was detected using diaminobenzidine tetrahydrochloride (DAB) containing 0.01% hydrogen peroxide and counterstaining with hematoxylin.…”

Section: Methodsmentioning

confidence: 99%

Distinct mechanisms of axonal globule formation in mice expressing human wild type α-synuclein or dementia with Lewy bodies-linked P123H ß-synuclein

et al. 2012

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BackgroundAxonopathy is critical in the early pathogenesis of neurodegenerative diseases, including Parkinson’s disease (PD) and dementia with Lewy bodies (DLB). Axonal swellings such as globules and spheroids are a distinct feature of axonopathy and our recent study showed that transgenic (tg) mice expressing DLB-linked P123H β-synuclein (P123H βS) were characterized by P123H βS-immunoreactive axonal swellings (P123H βS-globules). Therefore, the objectives of this study were to evaluate α-synuclein (αS)-immunoreactive axonal swellings (αS-globules) in the brains of tg mice expressing human wild-type αS and to compare them with the globules in P123H βS tg mice.ResultsIn αS tg mice, αS-globules were formed in an age-dependent manner in various brain regions, including the thalamus and basal ganglia. These globules were composed of autophagosome-like membranous structures and were reminiscent of P123H βS-globules in P123H βS tg mice. In the αS-globules, frequent clustering and deformation of mitochondria were observed. These changes were associated with oxidative stress, based on staining of nitrated αS and 4-hydroxy-2-nonenal (4-HNE). In accord with the absence of mitochondria in the P123H βS-globules, staining of nitrated αS and 4-HNE in these globules was weaker than that for αS-globules. Leucine-rich repeat kinase 2 (LRRK2), the PARK8 of familial PD, was detected exclusively in αS-globules, suggesting a specific role of this molecule in these globules.ConclusionsLysosomal pathology was similarly observed for both αS- and P123H βS-globules, while oxidative stress was associated with the αS-globules, and to a lesser extent with the P123H βS-globules. Other pathologies, such as mitochondrial alteration and LRRK2 accumulation, were exclusively detected for αS-globules. Collectively, both αS- and P123H βS-globules were formed through similar but distinct pathogenic mechanisms. Our findings suggest that synuclein family members might contribute to diverse axonal pathologies.

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Section: Methodsmentioning

confidence: 99%

Distinct mechanisms of axonal globule formation in mice expressing human wild type α-synuclein or dementia with Lewy bodies-linked P123H ß-synuclein

et al. 2012

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“…In situ hybridization histology, RT-PCR, and immunohistochemical studies indicate that PrRP is expressed in neurons of the nucleus tractus solitarius (NTS), the ventrolateral medulla (VLM), and in the caudal portion of the dorsomedial hypothalamic nucleus (DMN) (Figure 1) (Chen et al, 1999; Maruyama et al, 1999; Ibata et al, 2000; Lee et al, 2000). PrRP mRNA has also been found in a number of peripheral tissues, including the adrenal gland, pancreas, placenta, and testis (Fujii et al, 1999; Matsumoto et al, 1999a; Kalliomaki et al, 2004).…”

Section: Prrp and Gpr10 Expressionmentioning

confidence: 99%

“…PrRP-immunoreactive fibers are visible in many areas of the brain, such as the DMN, area postrema (AP), pontine parabrachial area, preoptic areas, bed nucleus of the stria terminalis (BNST), amygdala, mediodorsal nucleus of the thalamus, septal nucleus, and ependymal linings of the ventricles and blood vessels (Lin, 2008). One of the major projection sites is the paraventricular hypothalamus (PVN), where PrRP neurons appear to synapse directly on corticotrophin-releasing hormone (CRH) (Matsumoto et al, 1999a) and oxytocin neurons (Maruyama et al, 1999). Cell-specific connections also have been identified on magnocellular oxytocin/vasopressin neurons of the hypothalamic supraoptic nucleus (Maruyama et al, 1999), somatostatin neurons in the hypothalamic periventricular nucleus (Iijima et al, 2001), and on catecholaminergic cells of the adrenal medulla (Fujiwara et al, 2005).…”

Section: Prrp and Gpr10 Expressionmentioning

confidence: 99%

“…The sensation of satiety, and integration with descending motor pathways to regulate feeding, requires integration with higher brain centers. PrRP-immunoreactive fibers and GPR10 mRNA expression have been demonstrated in a number of hypothalamic nuclei (Fujii et al, 1999; Maruyama et al, 1999; Roland et al, 1999; Ibata et al, 2000; Lee et al, 2000). In particular, PrRP-containing neurons in the NTS project directly to the PVN (Onaka, 2004), where neurons containing CRH or oxytocin possess PrRP receptor (Lin et al, 2002a; Takayanagi and Onaka, 2010).…”

Section: Conserved Function Of Prrp and Gpr10 Signaling In Feeding Bementioning

confidence: 99%

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Physiological Roles of GPR10 and PrRP Signaling

Dodd¹,

Luckman²

2013

Front. Endocrinol.

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Prolactin-releasing peptide (PrRP) was first isolated from bovine hypothalamus, and was found to act as an endogenous ligand at the G-protein-coupled receptor 10 (GPR10 or hGR3). Although originally named as it can affect the secretion of prolactin from anterior pituitary cells, the potential functions for this peptide have been greatly expanded over the past decade. Anatomical, pharmacological, and physiological studies indicate that PrRP, signaling via the GPR10 receptor, may have a wide range of roles in neuroendocrinology; such as in energy homeostasis, stress responses, cardiovascular regulation, and circadian function. This review will provide the current knowledge of the PrRP and GPR10 signaling system, its putative functions, implications for therapy, and future perspectives.

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“…Prolactin-releasing peptide (PrRP) is produced in hypothalamic, thalamic and amyg dal oid nuclei, the pituitary gland and the bed nucleus of the stria terminalis (Maruyama, Matsumoto, Fujiwara, Kitada, Himuna, Onda, Fujino, & Inoue, 1999). It has been proposed as a mediator of stress responses (Maruyama et al, 2001;Adachi et al, 2005).…”

Section: Prolactin-releasing Peptidementioning

confidence: 99%

Influence of the estrous cycle on some non reproductive behaviors and on brain mechanisms in the female rat

Jatuff

Torrecilla

Quercetti

et al. 2012

Interd

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El rol de las hormonas sexuales sobre el comportamiento reproductivo ha sido extensamente documentado. La fluctuación periódica de hormonas sexuales en hembras de numerosas especies ha sido relacionada con cambios comportamentales no sexuales tales como humor, ansie dad, agresión y respuesta a estrés. El sustrato bioló-gico-neural de estos cambios se basa en los cambios que estas hormonas inducen en el Sistema Nervioso Central. Este trabajo resume algunos cambios que afectan a receptores y neurotrasmisores de los sistemas GABAérgico, serotoninér-gico, dopaminérgico y péptido liberador de prolactina del SNC de la rata. La rata hembra posee un ciclo sexual de 4 días de duración, denominado ciclo estrual. La presente revisión se informa en tres secciones. (1) Se presenta una breve descripción de la variación de tres hormonas sexuales principales con acción directa sobre el SNC: estrógenos (E2), progesterona (P4) y prolactina (PRL). Se han descripto propiedades ansiolíticas para P4 y anti-estrés para PRL; para E2, la bibliografía es controvertida, describiéndose acciones excitatorias y anti-estrés. (2) Se informan algunos cambios cerebrales y comportamentales que tienen lugar en cada estadio del ciclo estrual. Las fluctuaciones hormonales se consideran bá-sicas para la interpretación de tales cambios. (3) Se mencionan algunos hallazgos acerca de la influencia hormonal cíclica sobre los sistemas de neurotransmisión del SNC y sobre un nuevo pép-tido propuesto como mediador de la respuesta a INTERDISCIPLINARIA, 2012, 29, 1, 63-77 63 INFLUENCE OF THE ESTROUS ABSTRACTSex hormone fluctuations in females are involv ed in some behavioral states such as mood, anxiety, aggression and stress response, due to functional changes in the central nervous system (CNS) activity induced by the cyclic sex hormone fluctuation. This review includes three sections.1.-A description of the three major hormone fluctuations in the estrous cycle: estrogens (E2), progesterone (P4) and prolactine (PRL). E2 achieves the maximum circulation levels during P. E2 is mainly excitatory and has been considered to have an antidepressive action. The highest plasma levels of P4 and its metabolite allopregnenolone (ALLO) occur in P. Ovulation takes place in the night of P, and the resulting corpora lutea produces a secondary increase in P4 (and ALLO) on D. The P4 peak level occurring in D1 and in the evening of P was shown to exert benzodiazepine-like effects, including sedation. It has been proposed that ALLO, rather than P4 is the one acting on GABA systems. Circulating levels of ALLO parallel those of P4 across the estrous cycle and is known to have anxiolytic properties.PRL is produced mainly in the adeno hy pophysis, though synthesis in other sites of the brain also occurs. Its regulation is mostly inhibitory and is exerted by dopamine (DA) released in the hypothalamus. A surge in PRL secretion occurs during P. PRL would be a modulator of the HPA-axis, and is considered as a stress hormone.2.-Some behavioral and neural changes occur at each stage ...

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Immunocytochemical Localization of Prolactin-Releasing Peptide in the Rat Brain

Cited by 82 publications

References 7 publications

Distinct mechanisms of axonal globule formation in mice expressing human wild type α-synuclein or dementia with Lewy bodies-linked P123H ß-synuclein

Distinct mechanisms of axonal globule formation in mice expressing human wild type α-synuclein or dementia with Lewy bodies-linked P123H ß-synuclein

Physiological Roles of GPR10 and PrRP Signaling

Influence of the estrous cycle on some non reproductive behaviors and on brain mechanisms in the female rat

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