Immunocytochemical localization of neutrophil elastase in term placenta decidua and myometrium in pregnancy‐induced hypertension

Butterworth, Bridget; Greer, I. A.; Liston, W. A.; Haddad, N. G.; Johnston, Tracey

doi:10.1111/j.1471-0528.1991.tb13516.x

Cited by 59 publications

(27 citation statements)

References 11 publications

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“…These results coincide with previous studies demonstrating that choriodecidual leukocytes express CAMs [16,39,40], cytokines [8,41,42], chemokines [16,34], and their receptors [16,34,43], enzymes [35,44,45], antimicrobial peptides [46], markers of activation [17,39,[47][48][49], and even hormone receptors [50] before and during the process of labor. Together, these findings demonstrate that the choriodecidual leukocytes are a rich source of inflammatory mediators that can participate in the proinflammatory milieu that accompanies the process of labor.…”

Section: Resultssupporting

confidence: 81%

Choriodecidual leukocytes display a unique gene expression signature in spontaneous labor at term

et al. 2018

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Prior to and during the process of human labor, maternal circulating leukocytes infiltrate the maternal-fetal interface (choriodecidua) and become activated resembling choriodecidual leukocytes. Since, there is no evidence comparing maternal circulating and choriodecidual leukocytes, herein, we characterized their transcriptome and explored the biological processes enriched in choriodecidual leukocytes. From women undergoing spontaneous term labor we isolated circulating and choriodecidual leukocytes, performed microarray analysis (n = 5) and qRT-PCR validation (n = 9) and interaction network analysis with up-regulated genes. We found 270 genes up-regulated and only 17 genes down-regulated in choriodecidual leukocytes compared to maternal circulating leukocytes. The most up-regulated genes were CCL18, GPNMB, SEPP1, FN1, RNASE1, SPP1, C1QC, and PLTP. The biological processes enriched in choriodecidual leukocytes were cell migration and regulation of immune response, chemotaxis, and humoral immune responses. Our results show striking differences between the transcriptome of choriodecidual and maternal circulating leukocytes. Choriodecidual leukocytes are enriched in immune mediators implicated in the spontaneous process of labor at term.

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Section: Resultssupporting

confidence: 81%

Choriodecidual leukocytes display a unique gene expression signature in spontaneous labor at term

et al. 2018

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“…In fact, the findings suggest an enhanced capacity for all the steps in leukocyte/endothelial interaction, including capture, rolling, firm adhesion, and transmigration in preeclampsia. This may be the mechanism behind the increased infiltrate of neutrophils 19 and mononuclear perivascular cells 20 found in the decidua in preeclampsia.…”

Section: Discussionmentioning

confidence: 99%

Activation of Leukocytes During the Uteroplacental Passage in Preeclampsia

Mellembakken¹,

Aukrust²,

Olafsen³

et al. 2002

Hypertension

152

105

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Abstract-Endothelial dysfunction and inflammation appear to play a major role in the pathogenesis of preeclampsia. We hypothesize that a chronic inflammation in the decidua and placenta during preeclampsia may lead to a local leukocyte activation in this compartment. Venous blood was sampled simultaneously from antecubital and uterine veins during cesarean sections in 30 women with preeclampsia, 29 with uncomplicated pregnancies, and from 17 nonpregnant women. The expression of adhesion molecules and complement-related markers on neutrophils and monocytes was analyzed by flow cytometry. In patients with preeclampsia, neutrophil expression of the integrins CD11a, CD11b, and CD11c and of the complement related markers CD35 and CD59 was significantly higher in samples from uterine than from antecubital veins. No differences were found in nonpregnant women. On monocytes the expression of the Sialyl Lewis x antigen, the integrins CD11a, CD11c, and CD49d, and the complement-related markers CD46 and CD59 was higher in samples from uterine than from antecubital veins during preeclampsia, but not in uncomplicated pregnancies, whereas in nonpregnant women CD31 was decreased. Our findings suggest activation of neutrophils and monocytes taking place during the uteroplacental passage in preeclamptic, but not in normal pregnancies. Such a local inflammatory response involving enhanced leukocyte/endothelial interaction may contribute to the pathogenesis of this disorder.

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“…2 Immune abnormality has been speculated to lead to cellular activation and release of substances that alter maternal endothelial function in a direction that causes vascular leakage, coagulopathy, and hypertension. Evidence suggests that neutrophils are activated in both the placental bed 3 and maternal circulation of women with preeclampsia 4 -8 and that this activation resolves after delivery. 9 Neutrophil activation is associated with free radical release that either can affect endothelial function directly or contribute indirectly through production of lipid peroxides.…”

mentioning

confidence: 99%

Study of Plasma Factors Associated With Neutrophil Activation and Lipid Peroxidation in Preeclampsia

Barden

Ritchie²,

Walters³

et al. 2001

Hypertension

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Abstract-Neutrophil activation occurs in women with preeclampsia and is resolved after delivery. The present study examined whether circulating factors in plasma of women with preeclampsia caused neutrophil activation and lipid peroxidation. Twenty-one women with proteinuric preeclampsia were matched for age and gestational age with 19 normal pregnant women. Plasma was collected from all subjects before delivery and at 6 weeks postpartum and incubated with autologous white-cell buffy coat collected at the postpartum visit. Neutrophil activation was assessed by level of CD11b and CD18 expression after incubation with autologous antepartum or postpartum plasma. Lipid peroxidation was assessed by measurement of F 2 -isoprostanes in plasma, plasma-white cell incubates, and urine. Neutrophil CD11b and CD18 expression was not differentially altered by incubation with plasma from either women with preeclampsia or normal pregnant women and was similar between groups when incubation was performed with plasma collected after delivery. In preeclampsia, plasma F 2 -isoprostanes were significantly increased before and after delivery compared with controls. Plasma F 2 -isoprostanes were increased 2-fold after incubation of plasma with buffy coat, but preeclamptic women had higher levels compared with those of controls when either pregnant or postpartum plasma was used. In pregnant preeclamptics, plasma F 2 -isoprostanes were positively correlated with lymphocyte count. Six weeks after delivery, plasma F 2 -isoprostanes in the preeclamptic women were significantly positively associated with lymphocyte count and cholesterol and negatively associated with albumin. In conclusion, the present study does not suggest that a stable circulating factor causes neutrophil activation in preeclampsia. However, lipid peroxidation is elevated before and after delivery in women with preeclampsia, which suggests that these women may have an underlying predisposition to increased oxidative stress that may be driven by or contribute to a persistent low-grade inflammatory response. (Hypertension. 2001;38:803-808.)

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Immunocytochemical localization of neutrophil elastase in term placenta decidua and myometrium in pregnancy‐induced hypertension

Cited by 59 publications

References 11 publications

Choriodecidual leukocytes display a unique gene expression signature in spontaneous labor at term

Choriodecidual leukocytes display a unique gene expression signature in spontaneous labor at term

Activation of Leukocytes During the Uteroplacental Passage in Preeclampsia

Study of Plasma Factors Associated With Neutrophil Activation and Lipid Peroxidation in Preeclampsia

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