Immunocytochemical Localization of Lymphatic and Venous Vessels in Colonic Polyps and Adenomas

Abstract

Histopathological localization of lymphatic vessels has been hindered because of a lack of suitable immunocytochemical markers for lymphatic vessels. Using lymphatic vessels endothelial hyaluronan receptor-1 (LYVE-1) immunocytochemical staining, hyperplastic polyps, tubular adenomas to villous adenomas, were investigated for lymphatic vessels compared with immunostained blood vessels using factor-8. Four cases each of hyperplastic polyps, tubular adenomas to villous adenomas, were routinely fixed in formalin and embedded in paraffin and were immunostained using goat anti-LYVE-1 for lymphatic vessels and rabbit anti-factor-8 for blood vessels. In normal colon and hyperplastic polyps, slender lymphatic vessels were noted in muscularis mucosa, which spread into the base of colonic crypt, whereas round venous vessels, they extend into lamina propria. In tubular adenomas, small lymphatic and venous vessels were noted in broad fibrous stalks. In villous adenomas, smaller lymphatic and venous vessels were noted in fine intervillous stroma. In normal colon and hyperplastic polyps, slender, irregularly shaped lymphatic vessels were present in muscularis mucosa, spreading into the base of the colonic crypt. In tubular adenomas, small lymphatic and venous vessels were noted in fibrous stalks. In villous adenomas, smaller lymphatic and venous vessels were noted in intervillous stroma. There are no increased lymphatic and venous vessels in intermucosal stroma and stalks of adenomas compared with normal colon.

Tumor-Associated Lymphatic and Venous Vessels in Medullary Thyroid Carcinomas

“…It is very likely that formalin-fixation and paraffin-embedding masked or damaged the LYVE-1 epitope and/or collapsed lymphatic vessels. Therefore, it is very likely that LYVE-1 and F-8 immunostaining for MTCs was under detected by immunocytochemical staining with formalin-fixation and paraffin-embedding and more LYVE-1 and F-8 immunostaining would have been identified by adequate tissue processing as in frozen sections [13] [14].…”

“…Deparaffinized sections were treated with antigen retrieval procedure using citrate buffer pH 6.2. All the staining procedure was the same as previously reported including calcitonin [12]- [14] except for LYVE-1 and factor VIII (F-8) immunostaining: goat antihuman LYVE-1 (R & D System, Minneapolis, MN) was used at 1:100 dilution and F-8 immunostaining was performed using rabbit antihuman F-8 (Dako, Carpenteria, CA) diluted at 1:100 [12] [13]. In all cases, LYVE-1 and F-8 immunostaining was evaluated at the peri-and intratumoral tissues.…”

Cancer-Associated Lymphatic and Venous Vessels in Colonic Carcinomas

“…By immunocytochemical staining using lymphatic vessel endothelial hyluronan receptor-1 (LYVE-1) for lymphatic vessels and von Willebrand factor (factor-8, F-8) for venous vessels, lymphatic and venous vessels were concomitantly studied in surgically resected colonic cancer specimens since this immunocytochemical staining was previously used with colonic specimens harboring polyps and adenomas [13]. Recently, immunocytochemical staining for lymphatic vessels has been performed using Prox1, podoplanin, D2-40 and LYVE-1, the latter has been widely used as a reliable marker in the previous studies [7] [10]- [13].…”

“…Recently, immunocytochemical staining for lymphatic vessels has been performed using Prox1, podoplanin, D2-40 and LYVE-1, the latter has been widely used as a reliable marker in the previous studies [7] [10]- [13].…”

Cyclic Changes of Lymphatic and Venous Vessels in Human Endometrium

“…Using cryosections of human endometrium of menstrual, proliferative and secretary phase, this study aimed to unveil possible cyclic changes of venous and lymphatic vessels in the menstrual period, early proliferative, mid-secretary and late-secretary phase. For immunostaining, lymphatic vessel endotheliumhyarulonan receptor 1 (LYVE-1) was used for lymphatic vessels and von Willebr and factor (F-8) was used for venous vessels, respectively [4] [5]. Polyclonal LYVE-1 revealed more lymphatic vessels than using monoclonal D2-40 (Unpublished data).…”