Immunocytochemical localization of aromatase in ovaries of some rodents, cow and human.

Matsuda, Hiroshi; Fujita, Hisao; Ishimura, Kazunori; Osawa, Yoshio

doi:10.1267/ahc.17.311

Cited by 32 publications

(7 citation statements)

References 36 publications

(32 reference statements)

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“…However, no immunohistochemical localization has been reported in human ovaries to our knowledge. Immunohistochemical localization of P-450AROM *n *ne ovary has not been published except for one report showing the presence of P-450AROM in the theca interna (Matsuda et al, 1984). In addition, immunohistochemical localization of P-450AROM was reported in syncytiotrophoblast ofthe human placenta (Fournet-Dulguerov et al, 1987), and confirmed in the present study.…”

Section: Discussionsupporting

confidence: 85%

Immunolocalization of aromatase, 17 -hydroxylase and side-chain-cleavage cytochromes P-450 in the human ovary

et al. 1989

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Summary. Immunohistochemical localization of cholesterol side-chain-cleavage, 17\ g=a\ \ x=r eq-\ hydroxylase and aromatase cytochromes P-450 was performed in 35 morphologically normal human premenopausal ovaries by using specific antibodies against the enzymes. In well-developed ovarian follicles in the late stages of follicular growth, immunoreactivity of P-450arom was only seen in granulosa cells while P-45017\g=a\ and P-450scc activity was confined to theca interna cells, confirming that follicular oestrogen is produced in granulosa cells by the aromatization of androgens derived from the theca interna cells. In the corpus luteum, this functional differentiation is maintained, since immunoreactivity of P-450arom was exclusively present in luteinized granulosa cells while that of P-45017\g=a\ was present in luteinized theca cells. Immunoreactivity of P-450scc was present in both types of cells in the corpus luteum.

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Section: Discussionsupporting

confidence: 85%

Immunolocalization of aromatase, 17 -hydroxylase and side-chain-cleavage cytochromes P-450 in the human ovary

et al. 1989

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“…In other large antral follicles, a significant immunoreactivity was strictly localized in granulosa and absent in the theca interna. This could suggest a functional differentiation in the biosynthesis of estradiol; indeed, according to Matsuda et al [56], aromatase is expressed in these cells in many rodents; elsewhere, these observations coincide with the data of Irian et al and Tsafritrri et al [59,60] that described a maximal production of estradiol in the largest antral follicles.…”

Section: Discussionsupporting

confidence: 84%

“…In advanced preantral follicles, estradiol label was slight and uniform between theca and granulosa compartments, the labelling increased in antral follicles. The label expressed in theca cells would suggest the involvement of this component in biosynthesis of estradiol, as it is expressed in mouse and hamster theca cells which express aromatase [56] or in pigs [57]. If the estradiol observed in theca comes from granulosa, this feature reflects a paracrine regulation; indeed, estradiol causes an increase of androgen production by thecal cells and, contrarily, the suppression of progesterone production, throughout the 6-day culture period [58].…”

Section: Discussionmentioning

confidence: 99%

Morphometry and immunohistochemistry of follicles growth and steroidogenesis in saharian wild sand rat, Psammomys obesus, ovary.

Boubekri

Gernigon-Spychalowicz²,

Khammar³

et al. 2010

Folia Histochem Cytobiol.

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Abstract:The sand rat (Psammomys obesus) constitutes a model to study seasonal changes and several metabolic disorders. In order to perform breeding laboratory conditions, the reproductive function of this species living in North Occidental Algerian Sahara was studied. The aim of this work was to investigate the follicular growth changes and the steroidogenic associated aspects. The study was performed using morphometrical and immunohistochemical methods. From primordial to preantral states, the follicle diameter increased progressively from 17-20 μm to 192-225 μm. The preovulatory follicles reached about 500 μm in diameter. Immunoreactivity to progesterone, androstenediol and estradiol, varied in the different parts of the ovary and follicular cells. The progesterone antibody appeared clearly labelled in the theca interna of the growing follicle and increased in the granulosa; the androgen antibody was continuously weak and diffuses in all follicles; the estradiol labelling appeared weak and diffuse in preantral follicles then increased in antral follicles in both theca and granulosa or only in granulosa. In antral follicles, estradiol label was clearly localized in granulosa cells and totally devoid in theca cells. In Psammomys ovary, labels of hormone were diffuse or localized, weak or intense in the theca and or in the granulosa according to the follicle size.

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“…P450arom has been immunohistochemically localized in the syncytiotrophoblast of the human placenta (11)(12)(13)(14)(15), in ovaries of human, rat, mice, golden hamster, guinea pig, and cow (11,14,(16)(17)(18)(19)(20)(21)(22), in testes of rat, mouse, Supported in part by a grant from the Association pour la Recherche sur le Cancer (grant no. 6933) and by a grant from the Ligue Nationale Fransaise contre le Cancer (Comitt de la Manche).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical localization of cytochrome P450 aromatase in equine gonads.

Almadhidi

Seralini²,

Fresnel³

et al. 1995

J Histochem Cytochem.

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Estrogens are the major steroids produced by equine gonads.To identrfy the cells responsible for estrogen synthesis, an antiserum against purified equine testicular cytochrome P450 aromatase was produced in rabbits. The reactivity and specificity of the antiserum were assessed by ELISA, immuneblot analysis, and immunoneutralization studies. Immunofluorescence microscopy demonstrated that in the male gonad, cytochrome P450 aromtase (P45Oamm) was localized in the interstitial tissue, whereas, under the experimental conditions used, the Sertoli and germ cells did not show any specific staining. In the ovary, the granulosa cells of small follicles exhibited faint immunofluorescent staining for IntroductionThe conversion of androgens to estrogens, termed aromatization, is catalyzed by P45Oarom enzyme, the product of the gene called CYP19 in humans (1). Recently, Osawa et al. (2) showed that aromatase also acts as estrogen 2-hydroxylase and postulated that this enzyme could be responsible for the high levels of catechol estrogens and 19-hydroxyandrogens during pregnancy. The enzymatic complex, which is located in the endoplasmic reticulum, includes the heme glycoprotein P450arom and the flavoprotein NADPH-cytochrome P450 reductase.Estrogen biosynthesis occurs in several tissues and cells of mammals, including adipose tissue, skin fibroblasts, brain, pituitary, prostate, testis, ovary, placenta, and retina (3-10). P450arom has been immunohistochemically localized in the syncytiotrophoblast of the human placenta (11)(12)(13)(14)(15), in ovaries of human, rat, mice, golden hamster, guinea pig, and cow (11,14,(16)(17)(18)(19)(20)(21)(22), in testes of rat, mouse, Supported in part by a grant from the Association pour la Recherche sur le Cancer (grant no. 6933) and by a grant from the Ligue Nationale Fransaise contre le Cancer (Comitt de la Manche). and bear (23)(24)(25), in human prostate (6), in the brain of quail, rat, and mouse (26-28), and in the retina of goldfish (10).In mare, the concentration of estradiol-l7fl, which is the main estrogen in the follicular fluid, is greatly increased in the large follicles compared with the small follicles (8,29). The mare cyclic corpus luteum aromatizes androgens very efficiently (30), although only low levels of circulating estradioL17fl are present during the luteal phase (31,32). In comparison to other species (33,34), the stallion testis secretes very high amounts of estrogens, essentially as estrogen-3-sulfates. Plasma estrogens markedly increase with age (35), together with the number and volume of Leydig cells (36). However, the cellular localization of aromatase has not yet been established in equine ovary and testis.We have previously shown that equine aromatase exhibits some distinct properties compared with the human enzyme: the equine testicular, placental, and ovarian estrogen synthetase aromatizes androgens and 19-norandrogens with similar velocity, but not 16a-hydroxytestosterone or epitestosterone, in contrast to the human placental enzyme, which aromatizes androg...

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Immunocytochemical localization of aromatase in ovaries of some rodents, cow and human.

Cited by 32 publications

References 36 publications

Immunolocalization of aromatase, 17 -hydroxylase and side-chain-cleavage cytochromes P-450 in the human ovary

Immunolocalization of aromatase, 17 -hydroxylase and side-chain-cleavage cytochromes P-450 in the human ovary

Morphometry and immunohistochemistry of follicles growth and steroidogenesis in saharian wild sand rat, Psammomys obesus, ovary.

Immunohistochemical localization of cytochrome P450 aromatase in equine gonads.

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