Immunocytochemical localization of AMPA selective glutamate receptor subunits in the rat cochlea

Kuriyama, Hiromichi; Jenkins, Oliver H.; Altschuler, Richard A.

doi:10.1016/0378-5955(94)90114-7

Cited by 38 publications

(27 citation statements)

References 37 publications

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“…Several other neurotransmitter receptors have been reported to be expressed in both vestibular and cochlear hair cells, including AMPA receptors (Devau et al, 1993;Kuriyama et al, 1994;Demêmes et al, 1995;Matsubara et al, 1996;Knipper et al, 1997), the muscarinic receptor m3 (Safieddine et al, 1996), and ␣9 nicotinic receptors (Elgoyhen et al, 1994). None of these receptors is found solely expressed in IHCs of the organ of Corti.…”

Section: Discussionmentioning

confidence: 91%

“…Accumulating evidence suggests that fast excitatory synaptic transmission in the cochlea is mediated by an excitatory amino acid. The strongest evidence supporting this hypothesis is the expression of f unctional glutamate receptors by SGNs and their localization postsynaptic of the hair cell synapse (Bledsoe et al, 1981;Puel et al, 1991a,b;Ryan et al, 1991;Safieddine and Eybalin, 1992b;Kuriyama et al, 1994;Niedzielski and Wenthold, 1995). Although glutamate is present in hair cells, and its release from hair cells is stimulated by K ϩ in a Ca 2ϩ -dependent manner (Jenisson et al, 1985;Bobbin et al, 1990Bobbin et al, , 1991Kataoka and Ohmori, 1994; for review, see Eybalin, 1993), some studies suggest that a novel excitatory amino acid may be f unctioning at this synapse (Sewell and Morz, 1987).…”

Section: Abstract: Cochlea; Vestibular End Organ; Hair Cells; Spiralmentioning

confidence: 95%

“…Spiral and vestibular ganglion neurons express many glutamate receptors including AMPA, kainate, and NMDA ionotropic receptors and several metabotropic receptors (Ryan et al, 1991;Eybalin, 1992b, 1995;Demêmes et al, 1995;Niedzielski and Wenthold, 1995;Usami and Ottersen, 1995). Although only AMPA receptors have been shown to be postsynaptic to hair cells (Kuriyama et al, 1994;Demêmes et al, 1995;Usami and Ottersen, 1995;Matsubara et al, 1996), it is generally assumed that all of these receptors are expressed on the postsynaptic mem- brane of afferent fibers. AM PA and kainate receptors have been characterized pharmacologically (Billett et al, 1989;Nakagawa et al, 1991;Puel et al, 1991bPuel et al, , 1994Pujol et al, 1992;Deveaux et al, 1993), and some studies have identified f unctional NMDA receptors on SGNs (Felix and Ehrenberger, 1990;Puel et al, 1991a,b;Pujol et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

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The Glutamate Receptor Subunit δ1 Is Highly Expressed in Hair Cells of the Auditory and Vestibular Systems

Safieddine

Wenthold

1997

J. Neurosci.

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In the inner ear, fast excitatory synaptic transmission is mediated by ionotropic glutamate receptors, including AMPA, kainate, and NMDA receptors. The recently identified ␦1 and ␦2 glutamate receptors share low homology with the other three types, and no clear response or ligand binding has been obtained from cells transfected with ␦ alone or in combination with other ionotropic receptors. Studies of mice lacking expression of ␦2 show that this subunit plays a crucial role in plasticity of cerebellar glutamatergic synapses. In addition, these mice show a deficit in vestibular compensation. These findings and the nature of glutamatergic synapses between vestibulocochlear hair cells and primary afferent dendrites suggest that ␦ receptors may be functionally important in the inner ear and prompted us to investigate the expression of ␦ receptors in the cochlea and peripheral vestibular system. Reverse transcription and DNA amplification by PCR combined with immunocytochemistry and in situ hybridization were used. Our results show that the expression of ␦1 in the organ of Corti is intense and restricted to the inner hair cells, whereas ␦1 is expressed in all spiral ganglion neurons as well as in their satellite glial cells. In the vestibular end organ, ␦1 was highly expressed in both hair cell types and also was expressed in the vestibular ganglion neurons. The prominent expression of ␦1 in inner hair cells and in type I and type II vestibular hair cells suggests a functional role in hair cell neurotransmission. Key words: cochlea; vestibular end organ; hair cells; spiral ganglion neurons; PCR; in situ hybridization; Western blot; immunocytochemistryAuditory and vestibular stimuli are detected by hair cells in the inner ear and are transmitted to the brain by way of the auditory and vestibular nerves. Of the two types of hair cells found in the organ of Corti, inner hair cells (IHC s) and outer hair cells (OHC s), IHC s are the primary transducers of sensory information. IHC s form chemical synapses with dendrites of type I spiral ganglion neurons (SGNs) that constitute 90 -95% of the SGNs. OHC s synapse on the remaining type II SGNs (Spoendlin, 1972;Berglund and Ryugo, 1987). The vestibular epithelium contains two types of sensory hair cells. T ype I cells (V HCI) are flaskshaped and surrounded by an afferent nerve caly x, whereas type

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Section: Discussionmentioning

confidence: 91%

Section: Abstract: Cochlea; Vestibular End Organ; Hair Cells; Spiralmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

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The Glutamate Receptor Subunit δ1 Is Highly Expressed in Hair Cells of the Auditory and Vestibular Systems

Safieddine

Wenthold

1997

J. Neurosci.

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“…Although punctate immuno¯uorescence labeling was found for AMPA-type receptors around the OHC base (Kuriyama et al 1994), a lack of immunogold labeling was found in another study (Matsubara et al 1996). This suggests that glutamate receptors of this type, if they are present at all, are at low density.…”

Section: Fig 5 Graphs Based On the Preliminary Analysis Of Completementioning

confidence: 93%

“…There is also evidence that all spiral ganglion neurons, including those innervating OHCs, express a range of glutamate receptors (Niedzielski and Wenthold 1995). However, there are con¯icting reports as to whether the a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) type GluR4 receptor subunit found at IHC synapses is also present at those of the OHCs (Kuriyama et al 1994;Matsubara et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Distribution of the Glutamate/Aspartate Transporter GLAST in Relation to the Afferent Synapses of Outer Hair Cells in the Guinea Pig Cochlea

Furness

Hulme

Lawton

et al. 2001

JARO

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The glutamate/aspartate transporter GLAST is known to occur in the plasma membrane of supporting cells and their glialike processes around the synaptic region of inner hair cells of the mammalian cochlea. Its function there is presumably to take up glutamate following the release of this putative amino acid neuro transmitter from the inner hair cells. In this study, we have investigated whether GLAST is also associated with the outer hair cells using postembedding immunogold labeling. This is interesting because it is uncertain whether the outer hair cells have a functional synapse at which glutamate may be released. However, earlier ultrastructural studies of the afferent synapses in outer hair cells in several mammalian species have shown features normally associated with synaptic activity. These observations are con®rmed and extended here in guinea pig where these afferent synapses have presynaptic bodies, putative synaptic vesicles, and coated pits associated with them. Immunoreactivity for GLAST was found along the plasma membranes of Deiters' cells, especially around the synaptic region of the hair cell, on processes wrapped around approaching nerve ®bers. Semiquantitative analysis of the distribution of immunogold labeling of Deiters' cells con®rmed that it was densest in the region adjacent to the synapses. There was also more labeling in apical than in basal regions of the cochlea in three of the four animals examined, suggesting an association with the number of afferent synapses, which are more numerous in apical regions. Interestingly, labeling also occurred in other regions of the cell membrane away from the afferent terminals. This suggests that glutamate uptake is also required away from the immediate vicinity of synapses, perhaps as a consequence of glutamate dispersal resulting from the mechanical displacement of the cochlear partition during stimulation. Nonetheless, the particular association of GLAST with the synaptic region of the outer hair cell implies that the latter have active afferent synapses at which glutamate is released.

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Developmental regulation of neuron‐specific P2X₃ receptor expression in the rat cochlea

Huang

Greenwood

Thorne

et al. 2005

J of Comparative Neurology

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ATP-gated ion channels assembled from P2X3 receptor (P2X3R) subunits contribute to neurotransmission and neurotrophic signaling, associated with neurite development and synaptogenesis, particularly in peripheral sensory neurons. Here, P2X3R expression was characterized in the rat cochlea from embryonic day 16 (E16) to adult (P49-56), using RT-PCR and immunohistochemistry. P2X3R mRNA was strongly expressed in the cochlea prior to birth, declined to a minimal level at P14, and was absent in adult tissue. P2X3R protein expression was confined to spiral ganglion neurons (SGN) within Rosenthal's canal of the cochlea. At E16, immunolabeling was detected in the SGN neurites, but not the distal neurite projection within the developing sensory epithelium (greater epithelial ridge). From E18, the immunolabeling was observed in the peripheral neurites innervating the inner hair cells but was reduced by P6. However, from P2-8, immunolabeling of the SGN neurites extended to include the outer spiral bundle fiber tract beneath the outer hair cells. This labeling of type II SGN afferent fiber declined after P8. By P14, all synaptic terminal immunolabeling in the organ of Corti was absent, and SGN cell body labeling was minimal. In adult cochlear tissue, P2X3R immunolabeling was not detected. Noise exposure did not induce P2X3R expression in the adult cochlea. These data indicate that ATP-gated ion channels incorporating P2X3R subunit expression are specifically targeted to the afferent terminals just prior to the onset of hearing, and likely contribute to the neurotrophic signaling which establishes functional auditory neurotransmission.

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Immunocytochemical localization of AMPA selective glutamate receptor subunits in the rat cochlea

Cited by 38 publications

References 37 publications

The Glutamate Receptor Subunit δ1 Is Highly Expressed in Hair Cells of the Auditory and Vestibular Systems

The Glutamate Receptor Subunit δ1 Is Highly Expressed in Hair Cells of the Auditory and Vestibular Systems

Distribution of the Glutamate/Aspartate Transporter GLAST in Relation to the Afferent Synapses of Outer Hair Cells in the Guinea Pig Cochlea

Developmental regulation of neuron‐specific P2X₃ receptor expression in the rat cochlea

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Immunocytochemical localization of AMPA selective glutamate receptor subunits in the rat cochlea

Cited by 38 publications

References 37 publications

The Glutamate Receptor Subunit δ1 Is Highly Expressed in Hair Cells of the Auditory and Vestibular Systems

The Glutamate Receptor Subunit δ1 Is Highly Expressed in Hair Cells of the Auditory and Vestibular Systems

Distribution of the Glutamate/Aspartate Transporter GLAST in Relation to the Afferent Synapses of Outer Hair Cells in the Guinea Pig Cochlea

Developmental regulation of neuron‐specific P2X3 receptor expression in the rat cochlea

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Developmental regulation of neuron‐specific P2X₃ receptor expression in the rat cochlea