Immunocytochemical Localization and Immunological Characterization of Vitamin D-Dependent Calcium-Binding Protein in the Bullfrog Cerebellum

Gona, Amos G.; Pendurthi, T. K.; Al‐Rabiai, Suad; Gona, Ophelia; Christakos, Sylvia

doi:10.1159/000118679

Cited by 23 publications

(9 citation statements)

References 23 publications

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“…Interestingly, the two-stage process of cerebellar development in the bullfrog appears to be an intriguing parallel to the findings on chick-quail chimeras, where the cerebellum differentiates from contributions of both metencephalic and mesencephalic regions [Martinez and Alvarado-Mallart, 1989;Hallonet et al, 1990;Alvarez-Otero et al, 1993]. Since vitamin D-dependent calcium binding protein (CaBP, calbindin) not only appears specifically in Purkinje cells of various animals such as chicks [Roth et al, 1981], rats [Legrand et al, 1983], and the bullfrog [Gona et al, 1986], but also at an early stage when the Purkinje cells are still bipolar, a CaBP immunocytochemical study of the ventral cerebellar region of the metamorphosing cerebellum in tadpoles is a particularly valuable tool to shed light on the pattern of distribution and maturation of the second wave of Purkinje cells.…”

Section: Introductionmentioning

confidence: 72%

Calbindin Immunoreactivity in Purkinje Cells of the Bullfrog Cerebellum during Thyroxine-Induced Metamorphosis

Uray

Gona²,

Sexton³

1998

Brain Behav Evol

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Calbindin-immunoreactive Purkinje cells were identified in the cerebella of frog tadpoles that had been treated with thyroxine to accelerate metamorphosis. The dorsal part of the cerebellar plate contained the full complement of Purkinje cells which were all CaBP-immunoreactive, while in the ventral part of the cerebellum Purkinje cells acquired CaBP-immunoreactivity only after several days of thyroxine treatment. The ventral group of Purkinje cells was separated from the dorsal group by a distinct gap, which is the site of a shallow sulcus in adult frogs. Additionally, following thyroxine treatment, the numbers of CaBP-immunoreactive Purkinje cells in the ventral group were only half the numbers seen in frogs that metamorphosed spontaneously. We suggest that the variation in the CaBP-immunoreactivity of the dorsal and ventral groups of Purkinje cells, along with the gap in the Purkinje cell layer between the two groups, may be indicative of two distinct populations of Purkinje cells, with distinct patterns of generation, maturation, and perhaps, origin and connectivity, in the cerebellum of frogs.

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Section: Introductionmentioning

confidence: 72%

Calbindin Immunoreactivity in Purkinje Cells of the Bullfrog Cerebellum during Thyroxine-Induced Metamorphosis

Uray

Gona²,

Sexton³

1998

Brain Behav Evol

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“…In an earlier study, Gona et al [1986] observed CaBP immunoreactivity in mature Purkinje cells of the cerebellum in adults and tadpoles as well as in smaller cells in the auricular lobe region of tadpoles. The latter, small CaBP-immunoreactive cells were presumed to be immature Purkinje cells which were going to mature during metamorphosis.…”

Section: Introductionmentioning

confidence: 81%

Calbindin Immunoreactivity in the Auricular Lobe and Interauricular Granular Band of the Cerebellum in Bullfrogs

Uray

Gona²

1998

Brain Behav Evol

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Calcium binding protein (CaBP) immunoreactivity in the cerebellum of bullfrogs was examined, concentrating on cells associated with the auricular lobe. While anti-calretinin and anti-parvalbumin also immunoreacted with the same cell populations, anti-calbindin exhibited the most robust and typical pattern of immunostaining. Calbindin immunoreactivity was observed in various populations of cells in the auricular lobe and interauricular granular band of the cerebellum, in the cerebellar peduncle, and in a bundle of interauricular commissural fibers which course through the dorsal, marginal, part of the molecular layer. Cells in the granular layer of the ventral part (i.e., corpus cerebelli) of the cerebellar plate were not CaBP-immunoreactive, nor were any fibers in the molecular layer of this cerebellar region. We believe that axons of CaBP-immunoreactive granule-like cells of the auricular lobes contribute to the formation of the interauricular fiber bundle, which corresponds to the lateral commissure of urodele amphibians. The pattern of calbindin immunoreactivity in the auricular lobes and marginal part of the cerebellar plate provides additional evidence that this cerebellar compartment, which is already present in tadpoles, has a distinct origin, biochemical characterization and connectivity and is separate from the compartment that forms the corpus cerebelli of frogs during metamorphosis.

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“…Antibody to calbindin, the 28kDa calciumbinding protein that has been shown within the cerebellum to localize exclusively to Purkinje cells (Gona et al, 1986;Christakos et al, 1987) was used to verify the identity of the mutant Purkinje cells. Initial experiments were done with rabbit antisera from S. Christakos (Christakes et al, 1987), which was used at a primary antibody concentration of 1: 1000.…”

Section: Methodsmentioning

confidence: 99%

The Lurcher cerebellar mutant phenotype is not expressed on a staggerer mutant background

1991

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The hierarchy of the various processes responsible for the development of the complex, elaborated Purkinje cell can be examined by taking advantage of a series of spontaneous mutations that affect cerebellar development in the mouse. This study uses double mutants containing genes for two separate hereditary cerebellar mutations that have been shown to act intrinsically in Purkinje cells in order to investigate the time course and modes of action of these mutations. Lurcher mice show 100% degeneration of Purkinje cells, starting during the second postnatal week, while staggerer mice show reduced numbers of Purkinje cells in a distinctive mediolateral distribution from the time of birth, with the remainder grossly stunted. When these mutations are combined genetically, mice shown by progeny tests to harbor both staggerer and Lurcher genotypes exhibit staggerer-like behavior and overall cerebellar morphology; they also do not lose 100% of their Purkinje cells, as Lurcher mutants would otherwise do. Instead, they show a characteristic staggerer cerebellar pathology. We conclude that the intrinsic action of the staggerer gene in Purkinje cells occurs earlier in development than do effects of the Lurcher gene, and that the action of the staggerer gene prevents Purkinje cells from acquiring the characteristics required for the cytotoxic action of the Lurcher gene.

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Immunocytochemical Localization and Immunological Characterization of Vitamin D-Dependent Calcium-Binding Protein in the Bullfrog Cerebellum

Cited by 23 publications

References 23 publications

Calbindin Immunoreactivity in Purkinje Cells of the Bullfrog Cerebellum during Thyroxine-Induced Metamorphosis

Calbindin Immunoreactivity in Purkinje Cells of the Bullfrog Cerebellum during Thyroxine-Induced Metamorphosis

Calbindin Immunoreactivity in the Auricular Lobe and Interauricular Granular Band of the Cerebellum in Bullfrogs

The Lurcher cerebellar mutant phenotype is not expressed on a staggerer mutant background

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