Immunocytochemical localization and expression of heme oxygenase-1 in primary astroglial cell cultures during differentiation: effect of glutamate

Volti, Giovanni Li; Ientile, Riccardo; Abraham, Nader G.; Vanella, A.; Cannavò, Giuseppe; Mazza, Francesco; Currò, Monica; Raciti, G.; Avola, Roberto; Campisi, A.

doi:10.1016/j.bbrc.2004.01.090

Cited by 51 publications

(45 citation statements)

References 42 publications

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“…In particular, nuclear HO-1 localisation in astroglial cells was implicated in brain development and neurodegenerative diseases (Li Volti et al, 2004), in rat fetal lung cells exposed to hyperoxia as a chaperone or a nuclear messenger (Suttner et al, 1999) and in brown adipocyte as a transcription factor in adipogenesis (Giordano et al, 2000). Recently, HO-1 immunoreactive signal was detected in the nucleus of cultured cells after exposure to hypoxia and haeme, suggesting that this localisation may serve to upregulate genes that promote cytoprotection against oxidative stress (Lin et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…In response to haeme, these transcription factors bind to activation sequences of numerous genes encoding functions required for respiration and for controlling oxidative damage (Hon et al, 1999). As with other heat-shock proteins (Segui-Simarro et al, 2003), the transport of HO-1 could involve either interaction of the enzyme nuclear localisation signal with the nuclear pore complex or with other cytoplasmic components that would deliver the protein (Li Volti et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

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Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer

et al. 2007

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The role of oxidative stress in prostate cancer has been increasingly recognised. Acute and chronic inflammations generate reactive oxygen species that result in damage to cellular structures. Haeme oxygenase-1 (HO-1) has cytoprotective effects against oxidative damage. We hypothesise that modulation of HO-1 expression may be involved in the process of prostate carcinogenesis and prostate cancer progression. We thus studied HO-1 expression and localisation in 85 samples of organ-confined primary prostate cancer obtained via radical prostatectomy (Gleason grades 4 -9) and in 39 specimens of benign prostatic hyperplasia (BPH). We assessed HO-1 expression by immunohistochemical staining. No significant difference was observed in the cytoplasmic positive reactivity among tumours (84%), non-neoplastic surrounding parenchyma (89%), or BPH samples (87%) (P ¼ 0.53). Haeme oxygenase-1 immunostaining was detected in the nuclei of prostate cancer cells in 55 of 85 (65%) patients but less often in nonneoplastic surrounding parenchyma (30 of 85, 35%) or in BPH (9 of 39, 23%) (Po0.0001). Immunocytochemical and western blot analysis showed HO-1 only in the cytoplasmic compartment of PC3 and LNCaP prostate cancer cell lines. Treatment with hemin, a well-known specific inducer of HO-1, led to clear nuclear localisation of HO-1 in both cell lines and highly induced HO-1 expression in both cellular compartments. These findings have demonstrated, for the first time, that HO-1 expression and nuclear localisation can define a new subgroup of prostate cancer primary tumours and that the modulation of HO-1 expression and its nuclear translocation could represent new avenues for therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer

et al. 2007

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“…Considering the importance of Akt in myocardial homeostasis (34), the possibility that Akt and HO-1 may function in a similar fashion to exert cardioprotection is intriguing and merits further investigation. In addition, HO-1 translocates to the nucleus (33,45), raising the possibility that it may modulate nuclear events such as transcription and DNA binding. Thus the mechanism of HO-1 cardioprotection may involve both catalytic and noncatalytic processes.…”

Section: Discussionmentioning

confidence: 99%

Preemptive heme oxygenase-1 gene delivery reveals reduced mortality and preservation of left ventricular function 1 yr after acute myocardial infarction

Liu

Simpson²,

Brunt³

et al. 2007

American Journal of Physiology-Heart and Circulatory Physiology

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-We reported previously that predelivery of heme oxygenase-1 (HO-1) gene to the heart by adenoassociated virus-2 (AAV-2) markedly reduces ischemia and reperfusion (I/R)-induced myocardial injury. However, the effect of preemptive HO-1 gene delivery on long-term survival and prevention of postinfarction heart failure has not been determined. We assessed the effect of HO-1 gene delivery on long-term survival, myocardial function, and left ventricular (LV) remodeling 1 yr after myocardial infarction (MI) using echocardiographic imaging, pressure-volume (PV) analysis, and histomorphometric approaches. Two groups of Lewis rats were injected with 2 ϫ 10 11 particles of AAV-LacZ (control) or AAV-human HO-1 (hHO-1) in the anterior-posterior apical region of the LV wall. Six weeks after gene transfer, animals were subjected to 30 min of ischemia by ligation of the left anterior descending artery followed by reperfusion. Echocardiographic measurements and PV analysis of LV function were obtained at 2 wk and 12 mo after I/R. One year after acute MI, mortality was markedly reduced in the HO-1-treated animals compared with the LacZ-treated animals. PV analysis demonstrated significantly enhanced LV developed pressure, elevated maximal dP/dt, and lower end-diastolic volume in the HO-1 animals compared with the LacZ animals. Echocardiography showed a larger apical anterior-to-posterior wall ratio in HO-1 animals compared with LacZ animals. Morphometric analysis revealed extensive myocardial scarring and fibrosis in the infarcted LV area of LacZ animals, which was reduced by 62% in HO-1 animals. These results suggest that preemptive HO-1 gene delivery may be useful as a therapeutic strategy to reduce post-MI LV remodeling and heart failure.

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“…This protein can be translocated to the nucleus of differentiated astroglial cells (Li Volti et al, 2004), where it may participate in the regulation of heme metabolism. Since nuclear heme can activate a high molecular weight complex by altering the affinity of HapI to Hsp90, the translocation of HO-1 to the nucleus would be a factor in the regulation of different transcriptional factors (Li Volti et al, 2004). Subcellular localization of HO-1 has also been detected in cytoplasm, nuclear matrix, mitochondria, and peroxisomes of parenchymal and nonparenchymal liver cell populations (Immenschuh et al, 2003).…”

Section: Subcellular Localization Of Heme Oxygenasesmentioning

confidence: 99%

“…The faint immunoactivity of HO-1 proteins was primarily localized in the cytosol and the perinuclear region. As the primary culture was prolonged to 14 and 21 days, an abundant expression of HO-1 proteins in astrocytes was detected in the nucleus and nucleoli (Li Volti et al, 2004). It seems that the dynamic pattern of HO-1 expression in astroglial cells may give this protein an important role in brain development and neurodegenerative diseases (Li Volti et al, 2004).…”

Section: B Carbon Monoxide and Nervous Systemmentioning

confidence: 99%