Immunocytochemical Localisation of Transferrin in the Human Brain

Morris, Christopher M.; Candy, J.; Bloxham, C. A.; Edwardson, James A.

doi:10.1159/000147223

Cited by 41 publications

(18 citation statements)

References 8 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To characterize the inclusion‐bearing glial cells, anti‐GFAP was used as a marker for astrocytes and anti‐transferrin as a marker for oligodendroglia. In the central nervous system, transferrin is considered to be a reliable oligodendroglia cytoplasmic marker in grey and white matter [12,26,39]. In this study, transferrin‐immunoreactive cells were small with a perinuclear cytoplasm and short stubby processes, suggesting an oligodendroglial origine.…”

Section: Methodsmentioning

confidence: 53%

Widespread occurrence of argyrophilic glial inclusions in Parkinson's disease

Hishikawa

Hashizume

Yoshida

et al. 2001

Neuropathology Appl Neurobio

View full text Add to dashboard Cite

Argyrophilic glial inclusions, which are immunohistochemically positive for alpha-synuclein but negative for tau protein, were examined in the brain of Parkinson's disease (PD) patients. Autopsied brains of 10 individuals who died from PD, of two incidental Lewy body disease cases and of five age-matched individuals whose deaths were caused by non-neurological diseases were studied, histopathologically, by Gallyas-Braak staining and, immunohistochemically, with anti-alpha-synuclein antibody, anti-ubiquitin, and anti-tyrosine hydroxylase. All postmortem PD brains showed a significant number of argyrophilic glial inclusions, but no glial inclusions were found in control brains. The inclusions were found not only in the regions showing neuronal loss and gliosis, such as the substantia nigra, locus ceruleus and dorsal vagal nucleus, but also in regions without neuronal loss and gliosis, such as the cerebral cortex, cerebral white matter, striatum, globus pallidus, thalamus, cerebellum and spinal cord. The distribution and density of glial inclusions in PD brains varied from case to case but, in the cerebral cortex, the number of glial inclusions were fairly well correlated with the number of Lewy bodies. The distribution pattern of glial inclusions also showed a striking resemblance to that of catecholaminergic neurones and fibres. The abnormal accumulation of alpha-synuclein in glial cells was more widespread than neurone loss, and appears to be an important pathological feature of PD.

show abstract

Section: Methodsmentioning

confidence: 53%

Widespread occurrence of argyrophilic glial inclusions in Parkinson's disease

Hishikawa

Hashizume

Yoshida

et al. 2001

Neuropathology Appl Neurobio

View full text Add to dashboard Cite

show abstract

“…Dementia appeared within 1 year of the onset of parkinsonism in cases 2, 4, 6, and 7. Severe and fluctuating cognition, and impairment of visuospatial performance were observed in 4 of these 12 cases (cases 15,17,21,24). Clinical diagnosis was DLB in 3 cases, PD with dementia in 3 cases, and Shy-Drager syndrome in 2 cases (Table 1).…”

Section: Clinical and Pathological Correlationmentioning

confidence: 99%

Clinical and neuropathological correlates of Lewy body disease

et al. 2003

View full text Add to dashboard Cite

We investigated distribution of neuronal and glial inclusions in 30 brains obtained at autopsy from patients with Lewy bodies (LBs) disease, which was clinically diagnosed as Parkinson's disease (PD), dementia with Lewy bodies (DLB), or pure autonomic failure (PAF). The cases were classified, according to the guidelines for the pathological diagnosis of DLB, into three types: the neocortical type, the limbic type, and the brain stem-predominant type. All postmortem brains had coil-like glial inclusions as well as LBs, and the distribution pattern and density of glial inclusions and LBs varied. The distribution of glial inclusions was strikingly similar to that of LBs. In the cerebral cortex in particular, the number of glial inclusions was fairly well correlated with the number of LBs, irrespective of the three pathological types. In the brain stem, distribution was similar between glial inclusions and LBs, and there was no distinct pathological difference among the three types. Glial inclusions and LBs were immunohistopathologically similar with respect to ubiquitin, α-synuclein, and Gallyas-Braak staining. The clinical features of the three types of LB disease were also similar; i.e., parkinsonism, some dementia, and/or autonomic failure. The inclusions in neurons and glial cells occurred in parallel with respect to tissue distribution and immunohistochemical features, suggesting that accumulation of neuronal and glial inclusions in the LB diseases have a common pathological feature. Our findings suggest that DLB, PD with and without dementia, and PAF share one clinicopathological entity.

show abstract

“…Although the liver is the main site ofTf production (12), several other tissues including the brain (13)(14)(15)(16), testicular Sertoli cells (9). bone marrow (17).…”

Section: Discussionmentioning

confidence: 99%