Immunocytochemical investigation of catalase and peroxisomal lipid β-oxidation enzymes in human hepatocellular tumors and liver cirrhosis

Litwin, Jan A.; Beier, Konstantin; Völkl, Alfred; Hofmann, Walter; Fahimi, H. Dariush

doi:10.1007/s004280050432

Cited by 49 publications

(35 citation statements)

References 29 publications

(53 reference statements)

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“…The inhibition of catalase can have detrimental effects upon cell survival after oxidative stress. The catalase levels are significantly reduced in tumors of the liver (Litwin et al 1999) and other organs (Lauer et al 1999) as well as in a variety of pathological conditions, such as liver allograft rejection (Steinmetz et al 1998) and after ischemia-reperfusion injury (Singh 1996).…”

Section: Catalasementioning

confidence: 99%

Mammalian peroxisomes and reactive oxygen species

Schrader

Fahimi

2004

Histochem Cell Biol

155

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The central role of peroxisomes in the generation and scavenging of hydrogen peroxide has been well known ever since their discovery almost four decades ago. Recent studies have revealed their involvement in metabolism of oxygen free radicals and nitric oxide that have important functions in intra- and intercellular signaling. The analysis of the role of mammalian peroxisomes in a variety of physiological and pathological processes involving reactive oxygen species (ROS) is the subject of this review. The general characteristics of peroxisomes and their enzymes involved in the metabolism of ROS are briefly reviewed. An expansion of the peroxisomal compartment with proliferation of tubular peroxisomes is observed in cells exposed to UV irradiation and various oxidants and is apparently accompanied by upregulation of PEX genes. Significant reduction of peroxisomes and their enzymes is observed in inflammatory processes including infections, ischemia-reperfusion injury, and allograft rejection and seems to be related to the suppressive effect of tumor necrosis factor-alpha on peroxisome function and peroxisome proliferator activated receptor-alpha. Xenobiotic-induced proliferation of peroxisomes in rodents is accompanied by the formation of hepatic tumors, and evidently the imbalance in generation and decomposition of ROS plays an important role in this process. In PEX5-/- knockout mice lacking functional peroxisomes severe alterations of mitochondria in various organs are observed which seem to be due to a generalized increase in oxidative stress confirming the important role of peroxisomes in homeostasis of ROS and the implications of its disturbances for cell pathology.

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Section: Catalasementioning

confidence: 99%

Mammalian peroxisomes and reactive oxygen species

Schrader

Fahimi

2004

Histochem Cell Biol

155

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“…It has been demonstrated that human hepatocellular tumor cells contain fewer peroxisomes than extrafocal hepatocytes (48). Neoplastic transformation may affect the biogenesis of this organelle and might thus be responsible for some of the metabolic derangements observed in the disease processes of HCC.…”

Section: Members Of Heat Shock Protein 70 and 90 Familiesmentioning

confidence: 99%

Proteome Analysis of Hepatocellular Carcinoma by Two-dimensional Difference Gel Electrophoresis

Sun

Xing

Sun

et al. 2007

Molecular & Cellular Proteomics

222

172

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Hepatocellular carcinoma (HCC) is a highly malignant tumor, and chronic infection with hepatitis B virus is one of its major risk factors. To identify the proteins involved in HCC carcinogenesis, we used two-dimensional fluorescence DIGE to study the differentially expressed proteins in tumor and adjacent nontumor tissue samples. Samples from 12 hepatitis B virus-associated HCC patients were analyzed. A total of 61 spots were significantly up-regulated (ratio > 2, p < 0.01) in tumor samples, whereas 158 spots were down-regulated (ratio < ؊2, p < 0.01). Seventyone gene products were identified among these spots. Members of the heat shock protein 70 and 90 families were simultaneously up-regulated, whereas metabolismassociated proteins were decreased in HCC samples. The down-regulation of mitochondrial and peroxisomal proteins in these results suggested loss of special organelle functions during HCC carcinogenesis. Four metabolic enzymes involved in the methylation cycle in the liver were down-regulated in HCC tissues, indicating S-adenosylmethionine deficiency in HCC. Two gene products, glyceraldehyde-3-phosphate dehydrogenase and formimidoyltransferase-cyclodeaminase, were identified from inversely altered spots, suggesting that different isoforms or post-translational modifications of these two proteins might play different roles in HCC. For the first time, the overexpression of Hcp70/Hsp90-organizing protein and heterogeneous nuclear ribonucleoproteins C1/C2 in HCC tissues was confirmed by Western blot and then by immunohistochemistry staining in 70 HCC samples, suggesting their potential as protein tumor markers. In summary, we profiled proteome alterations in HCC tissues, and these results may provide useful insights for understanding the mechanism involved in the process of Proteomics analysis is currently considered to be a powerful tool for global evaluation of protein expression, and proteomics has been widely applied in analysis of diseases, especially in fields of cancer research. Quantitative protein expression profiling is a crucial part of proteomics, and such profiling requires methods that are able to efficiently provide accurate and reproducible differential expression values for proteins in two or more biological samples. Two-dimensional electrophoresis (2DE) 1 was a technique that was widely used for proteomics research. However, intergel variation and excessive time/labor costs have been common problems with standard 2DE. Two-dimensional (2D) DIGE might therefore be considered as one of the most significant advances in quantitative proteomics. Using the 2D DIGE approach, different samples prelabeled with mass-and charge-matched fluorescent cyanine dyes are co-separated in the same 2D gel, and an internal standard is used in every gel that has negated the problem of intergel variation (1). Moreover with the great sensitivity and dynamic range that is afforded by these dyes, 2D DIGE can give greater accuracy of quantitation than silver staining (2). It has been reported that the correlation betw...

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“…The tissue-specific expression of peroxisomal enzymes contributes substantially to the biochemical maturation of cells (15). The presence of peroxisomes has been cytochemically revealed in human breast, colon and liver carcinomas (15)(16) and a relationship is found between the specific activities of some peroxisomal enzymes and the histological tumour grades (15)(16).…”

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confidence: 99%

Lipid Metabolism Impairment in Human Gliomas: Expression of Peroxisomal Proteins in Human Gliomas at Different Grades of Malignancy

Benedetti

Galzio

Laurenti

et al. 2010

Int J Immunopathol Pharmacol

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Gliomas are histologically graded by cellularity, cytological atypia, necrosis, mitotic figures, and vascular proliferation, features associated with biologically aggressive behaviour. However, abundant evidence suggests the presence of unrecognized, clinically relevant subclasses of the diffuse gliomas, both in respect to their underlying molecular phenotype and their clinical response to therapy. It is wellknown that patient prognosis and therapeutic decisions rely on accurate pathological grading. Recently, it was reported that human gliomas accumulate lipid droplets during progression, suggesting a lipid metabolism impairment. Considering the crucial role of peroxisomes in lipid metabolism, in the present work we studied the expression profiles of proteins either exclusively localized to peroxisomes, such as peroxin14 (PEX14), peroxisomal membrane protein 70Kda (PMP70), acyl-CoA oxidase, thiolase, or partially associated to peroxisomes such as Hydroxymethylglutaryl-CoA reductase (HMGCoA-red) and peroxisomal-related proteins, namely PPARa, in human glioma specimens at different grades of malignancy. Moreover, Nile red staining of lipid droplets, thin layer chromatography (TLC) and proton nuclear magnetic resonance spectroscopy (NMR) were carried out in order to correlate the biochemical results with the lipid content of tumor tissues. The results obtained indicate that correlating the malignancy grade with the expression of peroxisomal genes and proteins, may constitute a sensitive tool to highlight possible subtypes not recognized by the classical histological techniques.

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Immunocytochemical investigation of catalase and peroxisomal lipid β-oxidation enzymes in human hepatocellular tumors and liver cirrhosis

Cited by 49 publications

References 29 publications

Mammalian peroxisomes and reactive oxygen species

Mammalian peroxisomes and reactive oxygen species

Proteome Analysis of Hepatocellular Carcinoma by Two-dimensional Difference Gel Electrophoresis

Lipid Metabolism Impairment in Human Gliomas: Expression of Peroxisomal Proteins in Human Gliomas at Different Grades of Malignancy

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