Immunocytochemical Evidence for the Presence of Prolactin in the Plerocercoid of Ligula Intestinalis (Cestoda: Pseudophyllidea)

Liu, Bo; Wakuri, Hidekazu; Mutoh, Ken-ichiro

doi:10.2535/ofaj1936.72.5_277

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…The reason for the difference between the result of the present and their study is probably partly due to the fact that the species of cestode and the specificity of antiserum were different. In our previous study we have in-formed that PRL immunoreactive bipolar or multipolar neurons have been extensively localizated in the peripheral nervous system, particularly in the transversal muscle layer and the medullary parenchyma, but there is a apparent lack of PRL-IR cells in the cerebral ganglia and the main nerve cords in the plerocercoid of Ligula intestinalis (Liu et al, 1995). The present study shows that there are some differences in the distribution of PRL immunoreactivity between the plerocercoid and the adult cestode.…”

Section: Discussionmentioning

confidence: 99%

“…PRL in the nervous system of cestodes seems to be a neglected field of research. In our previous study, the distribution of PRL immunoreactive neurons has been extensively observed in the peripheral nervous system (PNS) in the plerocercoid of Ligula intestinalis (Liu et al, 1995). The presence of PRL in the neurons can, of cauce, provide only indirect evidence of its function, but in view of that its physiologic activities in invertebrates is unknown, it is necessary to determine the patterns of the localization and innervation of PRL in cestodes.…”

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Prolactin in the Cestodes, Taenia solium and Taenia hydatigena

Liu

Wakuri

Mutoh

1996

Okajimas Folia Anatomica Japonica

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The demonstration of prolactin in cestodes, Taenia solium and Taenia hydatigena, was investigated by using immunocytochemical method. PRL immunoreactivity was widely distributed in the CNS and PNS in the worms. In the scolex PRL-IR perikarya occurred in the cerebral ganglia, around the basic area of the sucker and the rostral border region between the rostellum and the suckers with strong immunoreaction, distinctly in Taenia solium. A number of PRL-IR nerve fibres were observed in the main nerve cords, and a few fibres in the transverse nerve commissure. The holdfast musculature of the scolex was innervated by the PRL-IR fibres. A large number of PRL positive cells with processes and nerve plexuses were seen in the ootype/Mehlis' gland complex, oviduct, the duct of uterum, seminal receptacle and its associated duct, conspicuously in Taenia hydatigena. PRL-IR was evident in the wall of vas efferens of tests and vas deferens. A few PRL positive cells occurred in the medullary parenchyma amongst testes and sent their varicose processes to terminate to the testes. On the wall of the main excretory duct a few PRL-IR cells and fibres were seen.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Prolactin in the Cestodes, Taenia solium and Taenia hydatigena

Liu

Wakuri

Mutoh

1996

Okajimas Folia Anatomica Japonica

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“…Quintanar et al (2007a) found PRL in larval stages of the nematode Trichinella spiralis, in the nervous and reproductive systems of cestodes: Ligula intestinalis (Liu et al 1995), Taenia solium and Taenia hydatigena (Liu et al 1996), and it has been found in granules of neurons in insects: Leucophaena maderae (Veenstra et al 1985), Apis mellifera (Schmid et al 1990), Locusta migratoria and Sarcophaga bullata (Swinnen et al 1990).…”

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Immunodetection of Luteinizing Hormone (LH), Follicle-Stimulating Hormone (FSH), Thyroid Stimulating Hormone (TSH) and Prolactin (PRL) in Brachionus calyciflorus (Rotifera: Monogononta)

Alvarado-Flores¹,

Montoya-Garcia²,

Ventura-Juárez³

et al. 2008

RBT

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Abstract:The endocrine system controls and coordinates behavioral, biochemical, and physiological processes through signal mechanisms using neuropeptides or products of neurosecretory cells. Among invertebrates, this system is poorly studied in rotifers, in which estrogens and androgens significantly affect sexual reproduction. This is the first report of the presence of the Luteinizing Hormone (LH), Follicle-Stimulating Hormone (FSH), Thyroid Stimulating Hormone (TSH) and Prolactin (PRL) in rotifers. Analyses included the avidin-biotin-peroxidase complex method with primary antibodies LH (Anti-Rat LH serum for RiA), PRL (Anti-Rat PRL serum for RiA), FSH (Anti-Rat FSH serum for RiA) and TSH (Anti-Rat TSH serum for RiA). These hormones were found in females, males and parthenogenetic and sexual eggs of the freshwater Brachionus calyciflorus. The immunoreactivity of FSH, LH, TSH and PRL in females was observed in: ovaries, cerebrum, mastax, stomach, lorica, and the stomach gland. However, in males LH was observed only at the trochal disk and cerebrum. The hormones FSH, TSH and PRL, were observed in testicles, contractil vesicles, and cementary gland of males. Regarding amictic or parthenogenetic eggs, the hormones LH, FSH, TSH, and PRL were located mainly in the micromeres, and the staining in the macromeres was weak. On the other hand, in the mictic or sexual eggs the inner shell is stained for the hormones PRL and LH, opposite to the staining of FSH and TSH, located mainly in the embryo. in general, immuno-reactivity was observed in areas important for the reproductive, excretory, digestive and developmental processes. Rev. Biol. Trop. 57 (4):

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