Immunocytochemical demonstration of vertebrate neuropeptides in the earthworm Lumbricus terrestris (Annelida, Oligochaeta)

Curry, W.J.; Fairweather, Ian; Johnston, C.F.; Halton, D W; Buchanan, K. D.

doi:10.1007/bf00221468

Cited by 45 publications

(13 citation statements)

References 45 publications

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“…Immunoreactivity related to several vertebrate and invertebrate peptides, including neuropeptide Y, substance P, cholecystokinin, enkephalins, somatostatin, pancreatic polypeptides, bombesin, adrenocorticotropic hormone, gastrin, and proctolin (Sundler et al 1977;Alumets et al 1979;Aros et al 1980;Rzasa et al 1982Rzasa et al , 1984Renda et al 1987;Curry et al 1989;Lengvári et al 1992Lengvári et al , 1994 has also been demonstrated in the oligochaete Lumbricus terrestris. However, no data on the distribution of FMRFamide-like immunoreactivity in L. terrestris have yet been published.…”

Section: Introductionmentioning

confidence: 86%

“…Epidermal sensory cells containing noradrenaline, dopamine, met-and leu-enkephalin, pancreatic polypeptide, serotonin, and proctolin have been described in L. terrestris (Rude 1966;Ehinger and Myhrberg 1971;Gesser and Larsson 1986;Curry et al 1989;Lengvári et al 1992Lengvári et al , 1994. The importance of these peptides is not known, although the presence of FMRFamide-like immunoreactivity in approximately 5% of the epidermal sensory cells suggests that FMRFamide-related peptides may be involved in sensory processes in addition to the functions suggested above.…”

Section: Sensory Processesmentioning

confidence: 97%

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Distribution of FMRFamide-like immunoreactivity in the nervous system of Lumbricus terrestris

Reglődi

Slezák

Lubics

et al. 1997

Cell and Tissue Research

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The distribution of FMRFamide-like immunoreactive cell bodies and fibers in the nervous system of the earthworm Lumbricus terrestris has been studied by means of immunocytochemistry. The cerebral ganglion contains 150-170 immunoreactive nerve cells that are organized into six major groups in the rostral and five major groups in the caudal part of the ganglion; 160-180 immunoreactive nerve cells are present in the subesophageal ganglion, and 80-90 in the ventral cord ganglia. Immunoreactive neurons of the subesophageal and the ventral cord ganglia show similar distributions, in that FMRFamide-like immunoreactive cells form a ventromedial and a lateral cell group. Neuropil in all parts of the central nervous system shows intensively stained varicose and non-varicose fibers. Each segmental nerve contains FMRFamide-like immunoreactive fibers that can partly be traced to the two muscle layers of the body wall, and a fine immunoreactive network lies among the muscle fibers. A similar network is found in the wall of the alimentary canal. Immunopositive perikarya and fibers have been detected in the prostomial nerves, in the stomatogastric system. Some epithelial cells of the body wall are also immunopositive. The morphological characteristics and localization of FMRFamide immunoreactive neurons suggest that they may be involved in: (1) central integratory processes; (2) neuromuscular regulation in both the body wall and enteric system; (3) sensory processes.

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Section: Introductionmentioning

confidence: 86%

Section: Sensory Processesmentioning

confidence: 97%

Distribution of FMRFamide-like immunoreactivity in the nervous system of Lumbricus terrestris

Reglődi

Slezák

Lubics

et al. 1997

Cell and Tissue Research

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“…A number of vertebrate peptides and their homologous peptides have been suggested to be present in annelid tissues on the basis of immunohistochemical and radioimmunoassay studies (Alumets et al 1979;Carraway et al 1982;Dhainaut-Courtois et al 1985;Leake et al 1986;Curry et al 1989;Diaz-Miranda et al 1991;Salzet et al 1993a). Possible peptide regulation of gut motility has been suggested for several species of annelids.…”

Section: Introductionmentioning

confidence: 97%

Immunochemical demonstration of Eisenia tetradecapeptide, a bioactive peptide isolated from the gut of the earthworm Eisenia foetida , in tissues of the earthworm

Ukena

Oumi

Morishita

et al. 1997

Cell and Tissue Research

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The quantity and localization of Eisenia tetradecapeptide which was isolated from the gut of the earthworm Eisenia foetida were examined in tissues of the same species by enzyme-linked immunosorbent assay and immunohistochemistry. Analysis by enzyme-linked immunosorbent assay showed that Eisenia-tetradecapeptide-like immunoreactivity was present in both the central nervous system (cerebral ganglion, subesophageal ganglion, ventral ganglia, and ventral nerve cord) and the gut (esophagus, crop, gizzard, and intestine). The central nervous system contained a higher amount of Eisenia-tetradecapeptide-like immunoreactivity (1.3 pmol/mg wet weight) than the gut (0.2-0.6 pmol/mg wet weight). Eisenia-tetradecapeptide-like immunoreactivity was scarcely detected in the body-wall muscle, nephridia, and sexual organs (testis, ovary, seminal vesicle, and ovisac). Immunohistochemical analysis demonstrated that intense Eisenia-tetradecapeptide-like immunopositive cells and nerve fibers were present in the central nervous system. Immunoreactivity was found in the epithelial cells lining the esophagus and in the submucous plexus in various parts of the gut. Thus, the present study suggests that Eisenia tetradecapeptide is a neuropeptide and/or peptide hormone present in both the central nervous system and the gut of the earthworm and that its role involves the regulation of gut motility.

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“…The aim of this study was the investigation on the oocytes of abyssal teleost Hoplostethus mediterraneus in order to ascertain the presence of bioactive molecules too [9][10][11][12][13] and to confirm their role in the reproductive function.…”

Section: Introductionmentioning

confidence: 99%

Histological, histochemical and immunohistochemical study on the growing oocytes of the abyssal teleost Hoplostethus mediterraneus (V).

Calabrò¹,

Albanese²,

Bertuccio³

et al. 2008

Folia Histochem Cytobiol.

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Abstract:The oocytes of the abyssal Teleost, Hoplostethus mediterraneus were studied. Four stages of growth were observed and the oocytes of all the stages were surrounded by follicular cells and had several nucleoli in the nucleus. In the oocytes of the II° stage, vacuoles without contents, in oocytes of the III° stage several vacuoles with a basophilic contents and small yolk globules were identified. General and basic proteins, ribonucleoproteins, acid proteoglycans with -COOH groups were recognized in the cytoplasm, in the nucleoli of oocytes in the II° stage and in the vacuolar contents of oocytes in the III° stage. In the follicular cells, in the pellucid zone, in the yolk globules, from their beginning, glycoproteins were present. Positivity, for all lectins used, was revealed in the follicular cells and in the four stages of oocytes growth. α-D-glucose and α-D-mannose binding sites were in the pellucid zone and in the initial yolk globules. In the lather galactose and β-N-acetyl glucosamine were present too. nNOS and VIP immunopositivity revealed at the periphery of the cytoplasm and at network of nerve fibres between oocytes, suggests NO is involved in a mechanism of regulation of the gametogenesis and of the spawning.

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Immunocytochemical demonstration of vertebrate neuropeptides in the earthworm Lumbricus terrestris (Annelida, Oligochaeta)

Cited by 45 publications

References 45 publications

Distribution of FMRFamide-like immunoreactivity in the nervous system of Lumbricus terrestris

Distribution of FMRFamide-like immunoreactivity in the nervous system of Lumbricus terrestris

Immunochemical demonstration of Eisenia tetradecapeptide, a bioactive peptide isolated from the gut of the earthworm Eisenia foetida , in tissues of the earthworm

Histological, histochemical and immunohistochemical study on the growing oocytes of the abyssal teleost Hoplostethus mediterraneus (V).

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