Immunocytochemical Demonstration of the Synovial Membrane in Experimentally Induced Arthritis of the Rat Temporomandibular Joint.

Nozawa-Inoue, Kayoko; Takagi, Ritsuo; Kobayashi, Tatsuaki; Ohashi, Yasushi; Maeda, Takeyasu

doi:10.1679/aohc.61.451

Cited by 24 publications

(16 citation statements)

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“…The synovial lining layer shows an epithelium‐like arrangement in spite of the absence or incompleteness of adhesive structures between synovial type B cells (Nozawa‐Inoue et al,1998,1999b), giving rise to the question of how the lining cells form and keep their epithelium‐like arrangement. The present immunostaining showed intense CD44 immunoreaction in these type B cells whose peripheral ECM displayed HABP reaction.…”

Section: Discussionmentioning

confidence: 99%

“…The temporomandibular joint (TMJ), which is a bilateral diarthrosis between the temporal bone and the mandibular condyle, develops a synovial membrane. Many researchers agree with the notion that the synovial lining layer of the TMJ consists of macrophage‐like type A cells and fibroblast‐like type B cells (Graabæk,1984; Nozawa‐Inoue et al,1998,2003), as shown in the systemic joints (for review, see Iwanaga et al,2000). According to their ultrastructural configurations, the former cells absorb and degrade of various extracellular matrices and cell debris as well as antigens in the synovial fluid and synovial membrane (Okada et al,1981; Graabæk,1984; Athanasou,1995), whereas the latter cells serve in the synthesis and secretion of collagen (Visnapuu et al,2000), fibronectins (Mapp and Revell,1985), and hyaluronan (HA) (Roy and Ghadially,1967), all of which are the components of the synovial fluid as well as the extracellular matrices (ECMs) of the synovial membrane.…”

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confidence: 89%

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Localization of CD44 and hyaluronan in the synovial membrane of the rat temporomandibular joint

et al. 2006

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Previous studies have pointed out a lack of adhesion structures in the synovial lining layer of the rat temporomandibular joint (TMJ) despite showing an epithelial arrangement. CD44, a major cell adhesion molecule, plays crucial roles as an anchor between cells and extracellular matrices by binding hyaluronan (HA) for the development of organs or the metastasis of tumors. The present study examined the localization of CD44 in the synovial membrane of the rat TMJ by immunocytochemistry for OX50, ED1, and Hsp25, which are markers for the rat CD44, macrophage-like type A, and fibroblast-like type B synoviocytes, respectively. Histochemistry for HA-binding protein (HABP) was also employed for the detection of HA. OX50 immunoreactions were found along the cell surface and, in particular, accumulated along the surface of the articular cavity. Observations by a double immunostaining and immunoelectron microscopy revealed that all the OX50-immunopositive cells were categorized as fibroblastic type B cells, which had many caveolae and a few vesicles reactive to intense OX50. However, the macrophage-like type A cells did not have any OX50 immunoreaction in the synovial lining layer. A strong HABP reaction was discernable in the extracellular matrix surrounding both OX50-positive and -negative cells in the synovial lining layers, exhibiting a meshwork distribution, but weak in its sublining layer. This localization pattern of CD44 and HABP might be involved in the formation of the epithelial arrangement of the synovial lining layer. Furthermore, OX50 immunonegativity in the type A cells suggests their low phagocytotic activity in the rat TMJ under normal conditions. Anat Rec Part A, 288A: 646 -652, 2006.

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Section: Discussionmentioning

confidence: 99%

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confidence: 89%

Localization of CD44 and hyaluronan in the synovial membrane of the rat temporomandibular joint

et al. 2006

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“…A schematic drawing of the distribution of endothelial cells and macrophages in the developing rat TMJ. Graabaek, 1984;Nozawa-Inoue et al, 1998Iwanaga et al, 2000). Observation of the synovial membrane in osteopetrotic (op/op) mice showed a lack of type A cells (Naito et al, 1991), strongly suggesting that type A cells are differentiated from a monocyte lineage.…”

Section: Discussionmentioning

confidence: 99%

Development of the articular cavity in the rat temporomandibular joint with special reference to the behavior of endothelial cells and macrophages

et al. 2005

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Previous developmental studies on the temporomandibular joint (TMJ) have proposed several hypotheses on the formation of its articular cavity. However, detailed information is meager. The present study examined the formation process of the articular cavity in the rat TMJ by immunocytochemistry for CD31, RECA-1, and ED1, which are useful cellular markers for endothelial cells and monocyte/macrophage lineages, respectively. The upper articular cavity formation had begun by embryonic day 21 (E21) and was completed at postnatal day 1 (P1) in advance of the lower cavitation; the latter took place from P1 to P3. The occurrence and distribution pattern of the CD31-, RECA-1-, and ED1-positive cells differed between the upper and lower articular cavity-forming areas: the ED1-positive cells exclusively occurred in the area of the prospective upper articular cavity prior to its formation, while no ED1-positive cell appeared in the lower cavity-forming area. In contrast, the CD31-and RECA-1-positive endothelial cells were restricted to the lower cavity-forming area (never the prospective upper cavity) at E19 and diminished thereafter. Throughout the cavity formation, we failed to find any apoptotic cells in the cavity formation area, indicating no involvement of apoptosis in the cavity formation in TMJ. The present findings on the behaviors of endothelial cells and ED1-positive cells show a possibility of different mechanism in the cavity formation between the upper and lower articular cavities in the rat TMJ. The appearance of ED1-reactive cells and temporal vascularization may play crucial roles in the upper and lower articular cavity formation, respectively.

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“…Arthritic joints can be further categorized as having or not having an inflammatory exudate. Nozawa-Inoue et al (43) have demonstrated that induced arthritis is accompanied by joint effusion and synovial hypertrophy. In the human TMJ, effusion is strongly associated with disc displacement and OA changes (44).…”

Section: ␤mentioning

confidence: 99%

Amelioration of pain and histopathologic joint abnormalities in the Col1‐IL‐1β^XAT mouse model of arthritis by intraarticular induction of μ‐opioid receptor into the temporomandibular joint

Kyrkanides¹,

Fiorentino²,

Miller³

et al. 2007

Arthritis & Rheumatism

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Objective. To evaluate opioid receptor function as a basis for novel antinociceptive therapy in arthritis.Methods. We induced human -opioid receptor (HuMOR) expression in arthritic joints of mice, using the feline immunodeficiency virus (FIV) vector, which is capable of stably transducing dividing, growth-arrested, and terminally differentiated cells . Male and female Col1-IL-1␤ XAT -transgenic mice developed on a C57BL/6J background and wild-type littermates were studied.Results Conclusion. Our results indicate that prophylactic therapy with MOR overexpression in joints can successfully prevent the development of pain, dysfunction, and histopathologic abnormalities in the joints in arthritis. These findings may provide a basis for the future development of spatiotemporally controlled antinociceptive and antiinflammatory therapy for arthritis.Tissue injury caused by trauma, infection, and/or arthritis can produce inflammation, spontaneous pain, and hyperalgesia (1,2). In fact, according to US Public Health Service (PHS) estimates, Ͼ50 million Americans experience pain every year, with 45% seeking medical care at some point in their lives and 40% of pain patients never receiving adequate relief. Lipton et al (3) reported that 22% of the US population had experienced Ն1 episode of orofacial pain in the previous 6 months.

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Immunocytochemical Demonstration of the Synovial Membrane in Experimentally Induced Arthritis of the Rat Temporomandibular Joint.

Cited by 24 publications

References 19 publications

Localization of CD44 and hyaluronan in the synovial membrane of the rat temporomandibular joint

Localization of CD44 and hyaluronan in the synovial membrane of the rat temporomandibular joint

Development of the articular cavity in the rat temporomandibular joint with special reference to the behavior of endothelial cells and macrophages

Amelioration of pain and histopathologic joint abnormalities in the Col1‐IL‐1β^XAT mouse model of arthritis by intraarticular induction of μ‐opioid receptor into the temporomandibular joint

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Immunocytochemical Demonstration of the Synovial Membrane in Experimentally Induced Arthritis of the Rat Temporomandibular Joint.

Cited by 24 publications

References 19 publications

Localization of CD44 and hyaluronan in the synovial membrane of the rat temporomandibular joint

Localization of CD44 and hyaluronan in the synovial membrane of the rat temporomandibular joint

Development of the articular cavity in the rat temporomandibular joint with special reference to the behavior of endothelial cells and macrophages

Amelioration of pain and histopathologic joint abnormalities in the Col1‐IL‐1βXAT mouse model of arthritis by intraarticular induction of μ‐opioid receptor into the temporomandibular joint

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Amelioration of pain and histopathologic joint abnormalities in the Col1‐IL‐1β^XAT mouse model of arthritis by intraarticular induction of μ‐opioid receptor into the temporomandibular joint