Immunocytochemical demonstration of nerve growth factor (NGF) receptor in the pineal gland: effect of NGF on pinealocyte neurite formation

McNulty, John A.; Fox, Linda M.; Silberman, Simone

doi:10.1016/0006-8993(93)91223-f

Cited by 16 publications

(16 citation statements)

References 32 publications

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“…These sites all lack a blood-brain barrier. Within the pineal gland, p75 NTR -ir has been localized to a population of perivascular macrophage/microglial cells very similar in appearance to those found in the NL (McNulty et al 1993). Thus it is possible that p75 NTR is expressed by these cells at least partially in response to blood-borne factors, potentially including the NTs themselves, since the level of p75 NTR expression can be modulated by the amount of ligand available (Kumar et al 1993;Spoerri et al 1992).…”

Section: Discussionmentioning

confidence: 94%

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Upregulation of the p75 low-affinity neurotrophin receptor by phagocytically active perivascular cells in the rat neural lobe

Watt

Paden

2001

Cell and Tissue Research

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Expression of the p75 low-affinity neurotrophin receptor (p75NTR) was investigated immunocytochemically at the light and ultrastructural level during the axonal degeneration that follows partial denervation of the rat neural lobe (NL) and following systemic administration of lipopolysaccharide (LPS). A significant increase in the intensity and extent of p75NTR immunoreactivity in the NL of partially denervated animals compared with age-matched, sham-operated controls was observed at 5-10 days postdenervation, with immunoreactivity returning to control values by 35 days. Dual-label confocal comparison of p75NTR localization with that of the C3bi complement receptor, a microglial marker, and S100, an astrocyte-specific Ca2+-binding protein, revealed no colocalization. Immunoelectron-microscopic examination demonstrated that the p75NTR immunoreactivity is present in a subpopulation of cells located within the extensive perivascular space of the NL. No examples of p75NTR-immunoreactive pituicytes or endothelia were observed at the light or ultrastructural level. Dense p75NTR immunoreactivity was frequently observed surrounding endocytotic omega profiles of plasmalemma engulfing extracellular debris as well as lining vacuoles within the cytoplasm of perivascular cells. The association of p75NTR with phagocytosis was confirmed by confocal microscopy, showing the presence of p75NTR in all cells expressing the ED-1 antigen, which is restricted to the lysosomal membrane of phagocytes (Damoiseaux et al. 1994). Likewise, a marked increase in p75NTR and ED-1 immunoreactivity was observed in the NL following systemic administration of LPS. These results suggest a strong correlation between modulation of p75NTR immunoreactivity and conditions that induce high levels of phagocytic activity by perivascular cells in the NL of the rat. Implications for understanding the mechanisms by which phagocytes may support compensatory responses to neuronal injury are discussed.

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Section: Discussionmentioning

confidence: 94%

“…1) as well as in other circumventricular organs (Yan and Johnson 1989) and the pineal gland (McNulty et al 1993). These sites all lack a blood-brain barrier.…”

Section: Discussionmentioning

confidence: 98%

Upregulation of the p75 low-affinity neurotrophin receptor by phagocytically active perivascular cells in the rat neural lobe

Watt

Paden

2001

Cell and Tissue Research

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“…The addition of growth factors to the culture medium did not markedly affect process formation of pinealocytes, although it is known that NGF can promote the growth of processes in cultured rat pinealocytes (McNulty et al, 1993). Growth factors probably had no major impact in our system since the culture conditions already permitted a high degree of morphological differentiation of pinealocytes and we did not test the effects of these factors on cells maintained in serum-free medium.…”

Section: Discussionmentioning

confidence: 94%

“…Growth factors probably had no major impact in our system since the culture conditions already permitted a high degree of morphological differentiation of pinealocytes and we did not test the effects of these factors on cells maintained in serum-free medium. The presence of non-neuronal cells, which are a common constituent of many primary cultures of the pineal gland (e.g., Rowe et al, 1981;Araki et al, 1988;Rüppel and Olcese, 1991;McNulty et al, 1993McNulty et al, , 1995Welsh, 1994) and which have been reported to comprise a mixture of astrocyte-like glial cells, fibro- . Representative confocal optical sections (less than 0.8 µm thick) of pinealocytes double labelled with antibodies against synaptophysin and the transferrin receptor.…”

Section: Discussionmentioning

confidence: 99%

Expression of synaptic membrane proteins in gerbil pinealocytes in primary culture

Redecker¹,

Pabst²,

Gebert³

et al. 1997

J. Neurosci. Res.

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“…High KCl concentration induces membrane depolarization, which has been shown to increase the level of neurotransmitters in vitro (Hefti et al, 1982;Kessler, 1985;Garcia-Arraras, 1991). NGF appears to stimulate the growth of neurite processes in cultured pineal cells (McNulty et al, 1993). Our recent study showed that some other neurotrophic factors have similar neurogenic effects on cell differentiation of the rat pineal tissue as seen in elevated KCl concentration, although the molecular mechanisms involved are not known at the moment.…”

Section: Cellular Mechanism For Pineal Cell Differentiation: Analysismentioning

confidence: 95%

Developmental potency of cultured pineal cells: An approach to pineal developmental biology

Araki

2001

Microscopy Res & Technique

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The pineal organ is still an enigma in regard to its developmental and phylogenetic origin. Little is known of the mechanism involved in determination and differentiation of pineal cells and virtually no studies have been done on the induction and tissue interactions during pinealogenesis. Interest is also centered on the evolutional transformation in structure and function, which may be related to the developmental alterations in pineal morphogenesis between the lower and higher vertebrate species. For developmental studies, avian embryos have great advantages for various experimental manipulations, such as cell and organ culture, surgical operation, and in situ transfection of developmental genes. The present review describes our cell culture studies, which have been done on developing rat and quail pineal organs, in order to elucidate the developmental potency of pineal cells and the regulatory mechanism involved in the phenotypic expression of cell properties. A number of phenotypes including numerous neuron-specific substances are shown immunohistochemically to be expressed only under culture conditions, and not observed in the mature pineal organ. As development proceeds, some of the potencies for cell differentiation are lost; hence, in the mature pineal organs most neuronal phenotypes are not expressed. Numerous factors were discovered which affect phenotypic expression of cultured pineal cells in a cell-type-specific manner. These findings, together with immunohistochemical observations on developing pineal organs, reveal that the developing pineal organ is a unique and useful model system for developmental neurobiology and that cell culture techniques offer a powerful tool for the understanding of development and cell differentiation of this particular organ.

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Immunocytochemical demonstration of nerve growth factor (NGF) receptor in the pineal gland: effect of NGF on pinealocyte neurite formation

Cited by 16 publications

References 32 publications

Upregulation of the p75 low-affinity neurotrophin receptor by phagocytically active perivascular cells in the rat neural lobe

Upregulation of the p75 low-affinity neurotrophin receptor by phagocytically active perivascular cells in the rat neural lobe

Expression of synaptic membrane proteins in gerbil pinealocytes in primary culture

Developmental potency of cultured pineal cells: An approach to pineal developmental biology

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