Immunocytochemical demonstration of glucagon-like peptides in Mytilus edulis cerebral ganglia and an in vitro effect of vertebrate glucagon on glycogen metabolism

Kellner, K; Heude-Berthelin, Clothilde; Mathieu, Michel

doi:10.1016/s0040-8166(02)00020-4

Cited by 6 publications

(3 citation statements)

References 24 publications

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“…Previously, amino acids have been detected and quantified in freshwater and marine bivalves by Suzuki et al (1987). The presence of GnRH-like immunoreactive material in marine bivalve gonads (Pazos and Mathieu, 1999), detection of APGWamide-like immunoreactivity in scallops (Smith et al, 1997) and immunocytochemical demonstration of glucagon-like peptides in Mytilus edulis CNS (Kellner et al, 2002), all support the presence of peptide-like substances and their possible physiological role in the scallop. Furthermore, previously reported immunolocalization of FMRFamidecontaining neurons in the ganglia and gonad of the scallop P. maximus (Henry et al, 1995), detection of FMRFamide-like activity in P. magellanicus (Too et al, 1994) and the presence of GnRH-like immunoreactive material in bivalve gonads (Pazos and Mathieu, 1999) corroborate our data.…”

Section: Discussionmentioning

confidence: 74%

Identification of peptide-like substances in the Placopecten: Possible role in growth and reproduction

Pani¹,

Roghani²,

Croll³

2005

Biogenic Amines

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1. We have separated and quantified six putative peptide-like substances from the central nervous system (CNS), heamolymph, and gonads of the deep-sea scallop Placopecten magellanicus using high performance liquid chromatography with ultraviolet detection (HPLC-UV). 2. These peptide-like substances are tentatively identified as APGWamide, YFMRFamide, FMRFamide, nLRFamide, MNYLAFPRMamide and EDPFLRFamide. 3. All six peptide-like substances are co-eluted with known external peptide standards. Two of these compounds, APGWamide and FMRFaminde, are also confirmed for their identity by dot immunobinding assay (DIA). 4. The relative abundance of these peptide-like substances in the scallop is as follows: ganglia, greater than haemolymph, greater than gonads. 5. These compounds may be involved in growth and reproduction as demonstrated by their relative changes in HPLC-UV detected peak heights after thermal shockinduced spawning in the scallop.

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Section: Discussionmentioning

confidence: 74%

Identification of peptide-like substances in the Placopecten: Possible role in growth and reproduction

Pani¹,

Roghani²,

Croll³

2005

Biogenic Amines

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“…One of the final goals of our study is to obtain purified cell suspensions in order to develop homologous functional bioassays in oyster. At present, only haemocytes and storage cells of oysters can be maintained for a few days; these have been used, respectively, in immunological (Labreuche et al 2006;Lelong et al 2007) and energetic metabolism (Berthelin et al 2000;Kellner et al 2002;Hanquet-Dufour et al 2006) studies. Until recently, exploration of the functional role of molecules identified in oyster was only based on heterogeneous systems like rabbit chondrocytes to study the role of a chitinase (Badariotti et al 2006) or zebra fish embryos to explore the function of a receptor of the TGF-β family (Le Quéré et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Male germ cells of the Pacific oysterCrassostrea gigas: flow cytometry analysis, cell sorting and molecular expression

Franco

Kellner

Mathieu

et al. 2011

Aquat. Living Resour.

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-A technique was developed for dissection and isolation of male germ cells in the oyster Crassostrea gigas.This procedure can provide cells for the exploration of processes involved in the reproductive physiology of bivalves. Spermatogonia were chosen because of their essential role in spermatogenesis and the impact of gonia proliferation on reproductive effort. A non lethal method for determining sex and reproductive cycle stage was first validated in oysters. This first step was essential in order to constitute a homogeneous pool of oysters at the same stages of gametogenesis. Germ cell fractions were then obtained from a density gradient, and enrichment of each fraction was ratified by electron microscopy and by means of a 2-parameter flow cytometry procedure (DNA and mitochondrial staining). A significant enrichment in spermatogonia and spermatocytes was confirmed by the increased expression of markers of proliferative cells (proliferative cell nuclear antigen, PCNA) and early germ cells (oyster vasa-like gene). A preliminary cell sorting procedure is also reported, which was applied to fractions enriched in spermatogonia.

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“…Our data showed that the leech PCSK1 is prominently expressed in the supraoesophageal ganglion, representing the brain of the leech. Given that many invertebrate peptide hormones, including those of the leech, are produced by neurosecretory cells of the brain (Grimmelikhuijzen, et al 1980;Engelhardt, et al 1982;Vigna, et al 1984;Pestarino 1990;van Heumen and Roubos 1990;Gomot, et al 1992;Meester, et al 1992;Sonetti, et al 1992;Kellner-Cousin, et al 1994;Kellner, et al 2002;Wang, et al 2007;Miller and Newmark 2012), these specialized Hau-pcsk1-expressing cells may well be some of the neurosecretory cells of the leech.…”

Section: Invertebrate Pcsk1 Orthologs Have Not Been Characterized In mentioning

confidence: 99%

Diversification of metazoan Kexin-like proprotein convertases: insights from the leechHelobdella

Tao

Cheng

Song

et al. 2019

Preprint

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Intercellular communication is quintessential for multicellularity and often mediated by secreted peptide ligands. In Metazoa, proprotein convertases are a major class of endoproteases partaking in the proteolytic processing of these ligands, which is in turn required for their signaling activities. In vertebrates, the best-studied convertase substrates are neuropeptides, peptide hormones, and members of the TGFβ/BMP-family. Each ligand is processed by a particular subset of convertases. Therefore, the diversification of convertases may have contributed to the growing complexity of cellular communication in metazoan evolution.However, proprotein convertases have not been systematically explored in Metazoa. Here, we sampled the representative metazoan genomes and established that six Kexin-like proprotein convertases were present in the last common ancestor of protostomes and deuterostomes.Among these, we identified a novel PCSKX orthologous group (OG) that was lost in vertebrates. Spiralian protosomes have, in general, maintained all six OGs. Therefore, we characterized the functional divergence of the Kexin-like OGs in the leech Helobdella, an experimentally tractable spiralian. Gene expression patterns suggested that PCSK1 and PCSK2 are specialized for the processing of neuropeptides and peptide hormones in bilaterians and that the newly identified PCSKX is probably functionally similar to furin and PCSK7. Finally, we showed that, distinct from the BMP morphogen in vertebrate embryos, the convertase-mediated proteolytic cleavage is not required for the short-range BMP signaling in the dorsoventral patterning of leech ectoderm. Together, our data revealed the complexity of the Kexin-like proprotein convertase gene family and their roles in generating diverse patterns of cellular communication in Metazoa. 4 each individual convertase has distinct, preferred cleavage sites (Nelsen and Christian 2009), tissue-specific expression of convertase activity may be a critical factor in determining the differing strength and range of TGFβ/BMP signaling between tissue types.While it has been shown that the requirement for and consequence of convertasemediated cleavage differ between BMP ligands and between species (Fritsch, et al. 2012), the diversity in the roles of convertase-mediated cleavage in the regulation of cellular signaling has been largely unexplored. In the leech Helobdella, a novel form of short-range BMP signal patterns the dorsoventral axis of ectoderm (Kuo and Weisblat 2011b). Thus, to investigate the hypothesis that differential expression of selected Kexin-like proprotein convertases contributes to such a non-canonical deployment of this BMP signal, we set out to characterize the genes encoding proprotein convertases in the leech genome.Proprotein convertases have only been studied in a small number of mostly vertebrate model species, and the pre-vertebrate evolutionary history of this gene family is largely unknown. To provide a phylogenetic framework for our studies on the leech proprotein convertases a...

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Immunocytochemical demonstration of glucagon-like peptides in Mytilus edulis cerebral ganglia and an in vitro effect of vertebrate glucagon on glycogen metabolism

Cited by 6 publications

References 24 publications

Identification of peptide-like substances in the Placopecten: Possible role in growth and reproduction

Identification of peptide-like substances in the Placopecten: Possible role in growth and reproduction

Male germ cells of the Pacific oysterCrassostrea gigas: flow cytometry analysis, cell sorting and molecular expression

Diversification of metazoan Kexin-like proprotein convertases: insights from the leechHelobdella

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