Immunocytochemical Demonstration of Estrogen and Progesterone Receptors in Muscle Cells of Uterine Arteries in Rabbits and Humans*

Perrot-Applanat, M.; Groyer-Picard, Marie-Thérèse; Garcı́a, Esther; Lorenzo, Frédéric; Milgröm, Edwin

doi:10.1210/endo-123-3-1511

Cited by 178 publications

(67 citation statements)

References 41 publications

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“…ER has been visualized, using immunocytochemistry, in rat aortic smooth muscle cells (Orimo et al 1993) and in guinea pig, rabbit and human uterine arteries (Perrot-Applanat et al 1988, Leiberman et al 1990. In some other vascular segments, i.e.…”

Section: Introductionmentioning

confidence: 99%

Immunocytochemical demonstration of oestrogen receptor beta in blood vessels of the female rat

Andersson

Lydrup²,

Fernö³

et al. 2001

Journal of Endocrinology

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The role of oestrogen receptor (ER) in vascular function remains unclear. With the use of a specific ER antibody we have now, using immunocytochemistry, visualized ER in different parts of the vascular tree. In about 70% of medial smooth muscle cells of female rat aorta, tail artery and uterine artery, nuclear immunoreactivity to ER was observed. In these vessels endothelial cells also expressed ER . Vascular expression of the ER subtype was lower than that of ER . In aorta and tail artery, no immunoreactivity towards ER was observed, while in uterine vessels occasional medial smooth muscle and endothelial cells expressed this ER subtype. ER and expression in uterine vessels was independent of the stage of the oestrous cycle, suggesting that variations in uterine blood flow occurring during the cycle are independent of ER density. The regional distribution of ER , as determined by immunocytochemistry, was supported by measurements of ER levels by enzyme immunoassay. In the uterine artery, the level of ER was several times higher (P<0·001) than that of aorta and tail artery (10·1 1·7 fmol/mg protein in the uterine artery vs 3·3 1·0 and 0·5 0·5 fmol/mg protein in aorta and tail artery respectively). Thus, a prominent nuclear expression of ER was observed in the vascular wall of several parts of the vascular tree, while ER predominantly was expressed in uterine vessels, suggesting that ER and may have different roles in vascular function.

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Section: Introductionmentioning

confidence: 99%

Immunocytochemical demonstration of oestrogen receptor beta in blood vessels of the female rat

Andersson

Lydrup²,

Fernö³

et al. 2001

Journal of Endocrinology

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“…ods for studying the progesterone receptor in sections of paraffin-embedded tissue. Perrot-Applanat et al (16) have further examined the immunocytochemical localization of progesterone receptors in frozen sections from human breast tumors with a monoclonal antibody to this receptor and demonstrated that the progesterone receptors are accumulated in tumor cell nuclei. In this article we describe our results whichdemonstrate estradiol, progesterone and progesterone receptors in routine paraffinsections of human salivary glands and salivary adenoid cystic carcinomas, using an indirect immunoperoxidase technique.…”

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confidence: 99%

Immunohistochemical Localization of Estradiol, Progesterone, and Progesterone Receptor in Human Salivary Glands and Salivary Adenoid Cystic Carcinomas.

Ozono

Onozuka

Sato

et al. 1992

Cell Struct. Funct.

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Keywords:estradiol/progesterone/progesterone receptor/humansalivary gland/salivary adenoidcystic carcinomas ABSTRACT. Immunohistochemical analyses of estradiol, progesterone and progesterone receptor were carried out in human salivary gland and salivary adenoid cystic carcinoma. Immunoreactivity to estradiol and progesterone was found in cytoplasm of the cells of the excretory duct system within normal salivary glands, whereas the progesterone receptor was restricted to nuclei of the cells where both sex steroids were positive. In addition, we demonstrated the presence of both sex steroids and the receptor for progesterone in salivary adenoid cystic carcinomas. These data indicate that the human salivary gland is one of the target tissues of estrogen. This also suggests the good possibility that tumors which express progesterone receptors will respond to endocrine therapy.Humansaliva contains the steroid hormones estrogen and progesterone, which provide a basis for radioimmunoassay for the different types of salivary steroids (9, 18, 20). However, there is no evidence presently available to indicate the direct action of sex steroid hormones on humansalivary glands or salivary gland cancer. In addition, it is of great importance to know the exact localization of the two types of sex steroid hormonesto understand the function and metabolic properties of normal and tumor cells; however, there has been no previous attempt to directly identify estradiol and progesterone in microscopic sections of normal and neoplastic salivary glands. Kurmanet al. (8) in rats that progesterone receptors are present in cell nuclei of the intralobular duct system within submandibular glands (14). These findings suggest that the immunohistochemical technique, using specific antibodies against estradiol and progesterone or these receptors, makes possible investigation of this subject in the hor- (16) have further examined the immunocytochemical localization of progesterone receptors in frozen sections from human breast tumors with a monoclonal antibody to this receptor and demonstrated that the progesterone receptors are accumulated in tumor cell nuclei. In this article we describe our results whichdemonstrate estradiol, progesterone and progesterone receptors in routine paraffinsections of human salivary glands and salivary adenoid cystic carcinomas, using an indirect immunoperoxidase technique. MATERIALS AND METHODSThe following specimens were examined: 5 cases of primary adenoid cystic carcinoma and 6 specimens of normal salivary gland tissue (2 submandibular glands, 2 parotid glands and 2 minor salivary glands of the palate). The 5 patients included a 42-year-old manand 4 postmenopausal women,whose age at first diagnosis ranged from 61 to 82 (average 68) years (Table I).All samples were fixed in 3.7% buffered formalin (in 0.1 M sodium phosphate buffer, pH 7.2) for 24 hrs, and embedded in paraffin for diagnostic surgical pathology. In addition to routine hematoxylin and eosin stains, sections from representative paraffin blocks of...

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“…It is known that blood vessels express functional PgR (Perrot-Applanat et al 1988), which would be regulated by Pg as well as by synthetic progestins, like MPA (Sitruk-Ware 2000). The involvement of PgR in cellular and molecular effects of MPA and Pg at the vascular level has been reported.…”

Section: Discussionmentioning

confidence: 99%

Differential regulation of endothelium behavior by progesterone and medroxyprogesterone acetate

Cutini¹,

Campelo²,

Massheimer³

2013

Journal of Endocrinology

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Medroxyprogesterone acetate (MPA) is a synthetic progestin commonly used in hormone replacement therapy (HRT). The aim of this research was to study and compare the effect of progesterone (Pg) and MPA on the regulation of cellular events associated with vascular homeostasis and disease. Platelet adhesion to endothelial cells (ECs), nitric oxide (NO) production, and cell migration were studied using murine ECs in vitro exposed to the progestins. After 7 min of treatment, MPA significantly inhibited NO synthesis with respect to control values; meanwhile, Pg markedly increased vasoactive production. In senile ECs, the stimulatory action of Pg decreases; meanwhile, MPA maintained its ability to inhibit NO synthesis. The presence of RU486 antagonized the action of each steroid. When ECs were preincubated with PD98059 (MAPK inhibitor) or chelerythrine (protein kinase C (PKC) inhibitor) before Pg or MPA treatment, the former totally suppressed the steroid action, but the PKC antagonist did not affect NO production. In the presence of a PI3K inhibitor (LY294002), a partial reduction in Pg effect and a reversal of MPA action were detected. Using indomethacin, the contribution of the cyclooxygenase (COX) pathway was also detected. On platelet adhesion assays, Pg inhibited and MPA stimulated platelet adhesion to ECs. Under inflammatory conditions, Pg prevented platelet adhesion induced by lipopolysaccharide (LPS); meanwhile, MPA potentiated the stimulatory action of LPS. Finally, although both steroids enhanced migration of ECs, MPA exhibited a greater effect. In conclusion, the data presented in this research provide evidence of a differential regulation of vascular function by Pg and MPA.

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Immunocytochemical Demonstration of Estrogen and Progesterone Receptors in Muscle Cells of Uterine Arteries in Rabbits and Humans*

Cited by 178 publications

References 41 publications

Immunocytochemical demonstration of oestrogen receptor beta in blood vessels of the female rat

Immunocytochemical demonstration of oestrogen receptor beta in blood vessels of the female rat

Immunohistochemical Localization of Estradiol, Progesterone, and Progesterone Receptor in Human Salivary Glands and Salivary Adenoid Cystic Carcinomas.

Differential regulation of endothelium behavior by progesterone and medroxyprogesterone acetate

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