Immunocytochemical demonstration of dopamine-beta-hydroxylase and cytochrome B561 on the axonal reticulum in bovine sympathetic neurons.

Quatacker, J.; Annaert, Wim; Miserez, Bernard; Potter, W.P. De

doi:10.1177/40.10.1527378

Cited by 14 publications

(13 citation statements)

References 12 publications

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“…Also, at the light microscopical level the distribution of CGA-and cytochrome b,,,-IR in vas deferens is basically different from p38-IR. In addition, previous immunoelectron microscopic data clearly showed no colocalization of DPH and p38 on LDV in the bovine vas deferens (Quatacker et al, 1992). This is in agreement with the generally accepted idea that LDV and SSV form two distinct secretory pathways [see also reviews of De Camilli and Jahn (1990) and Matteoli et al ( 1991)…”

Section: Enrichment Of Sdvsupporting

confidence: 86%

“…Concerning the first finding, it has been demonstrated that two proteins present in LDV, i.e., DPH and cytochrome b561, are also present in the AR as visualized by a novel counterstaining technique (Quatacker et al, 1990(Quatacker et al, , 1992, confirming that the AR forms another amine-storing compartment (Tranzer, 1972). This membrane compartment extends from the trans-Golgi zone to the nerve terminal.…”

Section: Introductionmentioning

confidence: 94%

“…This membrane compartment extends from the trans-Golgi zone to the nerve terminal. The AR is anterogradely transported (Annaert et al, 1994) and differentiates locally in a tubular complex including vesiculotubular elements, LDV, and SDV (Quatacker et al, 1992). This led to the suggestion that these vesicles might also originate from the AR.…”

Section: Introductionmentioning

confidence: 99%

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Subcellular localization of synaptophysin in noradrenergic nerve terminals: A biochemical and morphological study

Annaert

Llona

Ridder

et al. 1995

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The subcellular localization of synaptophysin was investigated in noradrenergic nerve terminals of bovine vas deferens and dog spleen and compared with membrane-bound and soluble markers of noradrenergic storage vesicles. At the light microscopical level chromogranin A- and cytochrome b561-immunoreactivity revealed an identical and very dense innervation of the entire vas deferens. In the case of synaptophysin, most immunoreactivity was found only in the outmost varicosities closest to the lumen, which were also positive for chromogranin A. Small dense-core vesicles of dog spleen were purified using a combination of velocity gradient centrifugation and size exclusion chromatography. Small dense-core vesicles were enriched 64 times as measured by the noradrenaline content. Enrichments for dopamine-beta-hydroxylase were in a similar range. Synaptophysin-containing vesicles were smaller in size and they did not contain the typical noradrenergic markers dopamine-beta-hydroxylase, cytochrome b561, and noradrenaline. Instead, they might store adenosine triphosphate (ATP). A greater part of synaptophysin immunoreactivity was consistently found at high sucrose densities at the position of large dense-core vesicles. We conclude that in the noradrenergic nerve terminal: (1) small dense-core vesicles have a membrane composition similar to large dense-core vesicles, indicating that the former are derived from the latter, and (2) synaptophysin seems not to be present on small dense-core vesicles. We suggest the possibility that synaptophysin-containing vesicles form a residual population whose role in neurotransmission has been taken over by large and small dense-core vesicles following noradrenergic differentiation.

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Section: Enrichment Of Sdvsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Subcellular localization of synaptophysin in noradrenergic nerve terminals: A biochemical and morphological study

Annaert

Llona

Ridder

et al. 1995

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“…However, with regard to our further discussion, it should be emphasized that these results do not exclude that part of the NPY might still be present in another subcellular structure, such as, for example, the axonal reticulum. This subcellular organelle has already been demonstrated, by immunocytochemical methods, to contain NPY, DbH, and cytochrome b 561 (Quatacker et al, 1992(Quatacker et al, , 1993 and may have, as appears from gradient centrifugation studies on splenic nerve axons, an equilibrium density similar to that of axonal LDCV (De Potter, 1976).…”

Section: Analytical Subcellular Fractionation Studies Of a Dog Spleenmentioning

confidence: 87%

Noradrenergic neurons release both noradrenaline and neuropeptide Y from a single pool: The large dense cored vesicles

Potter

Partoens

Schoups

et al. 1997

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In peripheral adrenergic nerve endings, noradrenaline is stored in two different types of vesicles, the large and the small dense cored vesicles. A systematic study was undertaken to examine the release of noradrenaline and neuropeptide Y from dog spleen and rat vas deferens under various conditions of stimulation, particularly those which previously have demonstrated a differential regulation of exocytosis of the different types of storage vesicles. Here we present evidence that noradrenaline is released by exocytosis exclusively from the large dense cored vesicles, in which it is stored together with neuropeptide Y. Upon a single stimulation (at frequencies varying from 2-20 Hz), the release of noradrenaline and neuropeptide Y from the dog splenic nerve increased with the frequency of stimulation, but the ratio of noradrenaline to neuropeptide Y remained constant. After repeated stimulation of the splenic nerve, both substances' overflow decreased gradually and in parallel to values of 12.5% and 11.1% of the first stimulation for noradrenaline and neuropeptide Y, respectively. Similarly, repeated stimulation of the rat vas deferens (of which only 2-10% is large dense cored vesicles, whereas in the dog splenic nerve the large dense cored vesicles make up 30-40% of the total vesicle population) with 120 mM K+, in the presence of phentolamine, caused a gradual and parallel decline in the release of noradrenaline and neuropeptide Y (31.6% and 34.0%, respectively). Moreover, omega-conotoxin (10(-8) M to 10(-5) M) had a similar inhibitory effect on the release of both substances, alpha-latrotoxin (10(-9) M) evoked a parallel release of both noradrenaline and neuropeptide Y. The results indicate that noradrenaline in peripheral noradrenergic nerves is released exclusively from large dense cored vesicles by an exocytotic mechanism.

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“…Immunogold-silver particles also were associated with tubular membranes (Fig. 5B) that may represent the axonal reticulum, a membranous structure often found to be positive for LDCV markers (Quatacker et al, 1992;Annaert et al, 1994). Despite the high abundance of SSVs within synaptic profiles, immunogold-silver particles rarely were found to be affiliated with SSVs (Fig.…”

Section: The Cellular and Subcellular Localizations Of P64h1 In The Rmentioning

confidence: 88%

A 29 kDa Intracellular Chloride Channel p64H1 Is Associated with Large Dense-Core Vesicles in Rat Hippocampal Neurons

et al. 1999

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A novel class of intracellular chloride channels, the p64 family, has been found on several types of vesicles. These channels, acting in concert with the electrogenic proton pump, regulate the pH of the vesicle interior, which is critical for vesicular function. Here we describe the molecular cloning of p64H1, a p64 homolog, from both human and cow. Northern blot analysis showed that p64H1 is expressed abundantly in brain and retina, whereas the other members of this family (e.g., p64 and NCC27) are expressed only at low levels in these tissues. Immunohistochemical analysis of p64H1 in rat brain, using an affinity-purified antibody, revealed a high level of expression in the limbic system-the hippocampal formation, the amygdala, the hypothalamus, and the septum. Immunoelectron microscopic analysis of p64H1 in hippocampal neurons demonstrated a striking association between p64H1 and large densecore vesicles (LDCVs) and microtubules. In contrast, very low p64H1 labeling was found in perikarya or associated with small synaptic vesicles (SSVs) in axonal profiles. Immunoblot analysis confirmed that p64H1 is colocalized with heavy membrane fractions containing LDCVs rather than the fractions containing SSVs. These results suggest that p64H1-mediated Cl Ϫ permeability may be involved in the maintenance of low internal pH in LDCVs and in the maturation of LDCVs and the biogenesis of functional neuropeptides.

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Immunocytochemical demonstration of dopamine-beta-hydroxylase and cytochrome B561 on the axonal reticulum in bovine sympathetic neurons.

Cited by 14 publications

References 12 publications

Subcellular localization of synaptophysin in noradrenergic nerve terminals: A biochemical and morphological study

Subcellular localization of synaptophysin in noradrenergic nerve terminals: A biochemical and morphological study

Noradrenergic neurons release both noradrenaline and neuropeptide Y from a single pool: The large dense cored vesicles

A 29 kDa Intracellular Chloride Channel p64H1 Is Associated with Large Dense-Core Vesicles in Rat Hippocampal Neurons

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