Immunocompetence of the Severely Injured Spleen Verified by Differential Interference Contrast Microscopy: the Red Blood Cell Pit Test

Falimirski, Mark E.; Syed, Amjad; Prybilla, David

doi:10.1097/ta.0b013e3181507329

Cited by 15 publications

(6 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An analysis of 275 557 trauma admissions found hollow viscus injuries in association with only 0·3 per cent of blunt injuries, supporting the practice of non‐operative management49. Studies have confirmed long‐term preserved immunocompetence after conservative treatment of high‐grade splenic injury, and preservation should therefore be considered when resources allow adequate follow‐up, albeit with the risk of failure in mind50, 51.…”

Section: Failure Of Non‐operative Managementmentioning

confidence: 99%

Concise Synthesis of the Antidepressive Drug Candidate GSK1360707 by a Highly Enantioselective Gold‐Catalyzed Enyne Cycloisomerization Reaction

Teller

Fürstner

2011

Chemistry A European J

View full text Add to dashboard Cite

Out of depression: Indeed, no depressing results are obtained from a gold-catalyzed enyne cycloisomerization controlled by phosphoramidite ligands with TADDOL-related but acyclic backbones (see scheme; Cbz=benzyloxycarbonyl). The “triple-reuptake inhibitor” GSK1360707 was obtained in excellent yield and optical purity, therefore highlighting the relevance of asymmetric gold catalysis for practical applications

show abstract

Section: Failure Of Non‐operative Managementmentioning

confidence: 99%

Concise Synthesis of the Antidepressive Drug Candidate GSK1360707 by a Highly Enantioselective Gold‐Catalyzed Enyne Cycloisomerization Reaction

Teller

Fürstner

2011

Chemistry A European J

View full text Add to dashboard Cite

show abstract

“…However, it has to be noted that splenic infarcts after selective distal embolization rarely lead to clinical sequelae and splenic abscess can usually be treated percutaneously. Several studies have shown that PSAE and selective embolization have no major long-term impact on the splenic anatomy and immune function [42, 76, 77]. …”

Section: Diagnosticsmentioning

confidence: 99%

“…For that reason, the current recommendation is to treat patients after splenectomy with H. influenzae (Hib), pneumococcal, and meningococcal vaccination [78]. Several studies have shown that proximal ligation of the splenic artery and proximal and selective splenic embolization has no major long-term impact on the splenic anatomy and immune function [42, 76, 77]. Only one recent study suggests that the immunologic profile of embolized patients is reduced to controls.…”

Section: Diagnosticsmentioning

confidence: 99%

Literature Review of the Role of Ultrasound, Computed Tomography, and Transcatheter Arterial Embolization for the Treatment of Traumatic Splenic Injuries

Vlies

Delden

Punt

et al. 2010

Cardiovasc Intervent Radiol

View full text Add to dashboard Cite

IntroductionThe spleen is the second most frequently injured organ following blunt abdominal trauma. Trends in management have changed over the years. Traditionally, laparotomy and splenectomy was the standard management. Presently, nonoperative management (NOM) of splenic injury is the most common management strategy in hemodynamically stable patients. Splenic injuries can be managed via simple observation (OBS) or with angiography and embolization (AE). Angio-embolization has shown to be a valuable alternative to observational management and has increased the success rate of nonoperative management in many series.DiagnosticsImproved imaging techniques and advances in interventional radiology have led to a better selection of patients who are amenable to nonoperative management. Despite this, there is still a lot of debate about which patients are prone to NOM.Angiography and EmbolizationThe optimal patient selection is still a matter of debate and the role of CT and angio-embolization has not yet fully evolved. We discuss the role of sonography and CT features, such as contrast extravasation, pseudoaneurysms, arteriovenous fistulas, or hemoperitoneum, to determine the optimal patient selection for angiography and embolization. We also review the efficiency, technical considerations (proximal or selective embolization), logistics, and complication rates of AE for blunt traumatic splenic injuries.

show abstract

“…The CD20 + CD21 + B lymphocyte, which worked as an important producer of IgM, is mainly localized in the spleen (22). It has been reported that the level of IgM would be decreased in patients with splenectomy after trauma (6). Furthermore, there were no significant difference in WBC, RBC, PLT, and Hgb level among the three groups.…”

Section: Discussionmentioning

confidence: 99%

“…We presumed that in our MI model, there was no organic injury to the worked as an important producer of IgM, is mainly localized in the spleen (22). It has been reported that the lungs; therefore, there was no appropriate microenvironment for the activation of milieu-dependent differentialevel of IgM would be decreased in patients with splenectomy after trauma (6). Furthermore, there were no tion of MSCs.…”

Section: Statistical Analysis Assessment Of Liver and Spleen Functionmentioning

confidence: 99%

Impact of Escaped Bone Marrow Mesenchymal Stromal Cells on Extracardiac Organs after Intramyocardial Implantation in a Rat Myocardial Infarction Model

et al. 2010

View full text Add to dashboard Cite

Cell escape occurs after intramyocardial injection for treatment of myocardial infarction (MI) and then the migrated cells might be entrapped by extracardiac organs. We investigated the fate of migrated bone marrowderived mesenchymal stromal cells (MSCs) and their impact on lung, liver, and spleen. MI model was created by coronary artery ligation in female Lewis rats. Three weeks after the ligation, bromodeoxyuridine (BrdU)-labeled male MSCs were directly injected into the infarcted area in the cell transplantation group (n = 22). The same volume of phosphate-buffered solution (PBS) was injected in the control group (n = 21). In the sham group (n = 10) intramyocardial injection of the same volume of PBS was performed in healthy rats. Four weeks later, echocardiography was performed and the cell retention was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). Immunohistochemistry study was performed to identify the migrated cells. Heart function was improved after the cell injection. qRT-PCR results showed the percentage of retained cells in heart, spleen, liver, and lung ranked 3.63 ± 0.48%, 0.77 ± 0.13%, 0.68 ± 0.10%, 0.62 ± 0.11%, respectively, after cell transplantation. The implanted MSCs that escaped to liver, spleen, and lung did not differentiate into fibroblast, myofibroblast, or alveolar epithelial cells. However, the migrated MSCs in liver expressed functional hepatocyte marker. In conclusion, cell migration after intramyocardial injection did not result in deterioration of lung, liver, and spleen function. Our study might pave the way for new safety investigation of emerging cell resources and their impact on target and untargeted organs.Key words: Bone marrow mesenchymal stromal cells; Myocardial infarction; Lung; Liver; Spleen INTRODUCTIONfilter organs in pulmonary or systemic circulation (7,9, 27). Until now, little is known about the impact of escaped progenitor cells on extracardiac organs. ThereBone marrow-derived mesenchymal stromal cells (MSCs), which contain multipotent mesenchymal stem fore, in the present study we address 1) the cell retention after intramyocardial injection in a chronic MI model cells, have been a popular cell resource for cardiac regeneration in recent years (18). The therapeutic potency and 2) the fate and impact of escaped MSCs on liver, spleen, and lung. of MSC transplantation for the treatment of myocardial infarction (MI) has been well demonstrated in experi-MATERIALS AND METHODS mental and clinical studies. Intramyocardial injection is Animals considered as the most effective route for the precise cell delivery to the target areas. However, many cells Seventy syngenic Lewis rats (Vital River Laboratory Animal, Inc., Beijing, China) weighing 200-250 g were still migrated from the injection sites to the extracardiac organs, and these cells tended to be entrapped by the enrolled in this study. All animals received humane careReceived rats were sacrificed and the lung tissue homogenized. Cell CultureTumor necrosis factor-α (TNF-α) and interleukin-...

show abstract

Immunocompetence of the Severely Injured Spleen Verified by Differential Interference Contrast Microscopy: the Red Blood Cell Pit Test

Cited by 15 publications

References 31 publications

Concise Synthesis of the Antidepressive Drug Candidate GSK1360707 by a Highly Enantioselective Gold‐Catalyzed Enyne Cycloisomerization Reaction

Concise Synthesis of the Antidepressive Drug Candidate GSK1360707 by a Highly Enantioselective Gold‐Catalyzed Enyne Cycloisomerization Reaction

Literature Review of the Role of Ultrasound, Computed Tomography, and Transcatheter Arterial Embolization for the Treatment of Traumatic Splenic Injuries

Impact of Escaped Bone Marrow Mesenchymal Stromal Cells on Extracardiac Organs after Intramyocardial Implantation in a Rat Myocardial Infarction Model

Contact Info

Product

Resources

About