Immunochromatographic<i>Brucella-</i>Specific Immunoglobulin M and G Lateral Flow Assays for Rapid Serodiagnosis of Human Brucellosis

Smits, Henk L.; Abdoel, Theresia H.; Solera, J; Clavijo, Encarnación; Díaz, Ramón

doi:10.1128/cdli.10.6.1141-1146.2003

Cited by 100 publications

(110 citation statements)

References 24 publications

(16 reference statements)

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“…This could be an important aspect in countries such as Egypt where the disease is widely spread. Furthermore, our results are in agreement with the high sensitivity and specificity observed for the flow assay by earlier reports from Spain [19], Turkey [21,22], and Kazakhstan [21]. Therefore, in the absence of the laboratory support, the flow assay may be used for the confirmation of brucellosis in febrile patients with clinical suspicion of brucellosis by testing a drop of blood collected by finger prick.…”

Section: Discussionsupporting

confidence: 79%

“…For the lateral flow assay, 5 µl of serum followed by 130 µl of running fluid were added to the sample application pad in the sample well of the plastic assay device (Royal Tropical Institute, Amsterdam, The Netherlands) [19]. The assay was read after 10 to 15 minutes by visual inspection for staining of the test and control lines in the assay window of the device.…”

Section: Laboratory Testingmentioning

confidence: 99%

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Laboratory diagnosis of human brucellosis in Egypt and persistence of the pathogen following treatment

Marei

Boghdadi

Abdel-Hamed

et al. 2011

J Infect Dev Ctries

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Introduction: Brucellosis is a major public health problem in Egypt. The Brucella IgM/IgG lateral flow assay was developed as a point-ofcare test for the diagnosis of human brucellosis. The aim of this study was to assess the diagnostic value of the lateral flow assay for use in Egypt. Methodology: Fifty samples of patients who presented with clinical suspicion of brucellosis over a one-year period were collected. All samples were subjected to the Brucella IgM/IgG lateral flow assay, serum agglutination test (SAT), rose bengal RB Test (RB), 2-mercapteoethanol (2-ME), culture and PCR. SAT, 2-ME, culture and PCR were retested after the end of the treatment. Results: Culture and SAT confirmed the diagnosis of brucellosis in twenty patients. While 90% of the samples were positive by SAT, only 30% and 85% were positive by culture and PCR respectively. The sensitivity of the lateral flow assay calculated for the Brucella IgM/IgG was 95% and specificity was 97%. Conclusion: These data show that the lateral flow assay is more suitable for diagnosis of brucellosis in Egypt than culture and SAT. Application of the PCR on serum samples collected during follow-up revealed that the DNA of the pathogen was yet not completely cleared almost 60 days after the start of treatment with doxycycline and ciprofloxacin.

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Section: Discussionsupporting

confidence: 79%

Section: Laboratory Testingmentioning

confidence: 99%

Laboratory diagnosis of human brucellosis in Egypt and persistence of the pathogen following treatment

Marei

Boghdadi

Abdel-Hamed

et al. 2011

J Infect Dev Ctries

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“…Clinical features of the 13 patients of brucellosis were typical of the disease (Table 4). Three newly (5), sweats (9), myalgia (11), and arthralgia (18). ‡Any of the screening tests positive.…”

Section: Resultsmentioning

confidence: 99%

“…However, previous studies have shown that even in endemic regions the LFA IgM/IgM test has a sensitivity and specificity of~ 96%, even when used in endemic areas. 18,33,34 The performance of screening agglutination tests (either the RB or slide agglutination test) occasionally is inferior to the tube agglutination test, which has different incubation times and conditions. 35 Confirmatory serologies performed in this study included the tube agglutination test (with a 1 of 160 titer cutoff) and the 2-ME test (that differentiates IgM from IgG antibodies, indicating acute infection) to enhance the specificity of testing; previous studies from Peru and elsewhere have shown these criteria to be strongly suggestive of a new episode of brucellosis.…”

Section: Discussionmentioning

confidence: 99%

“…The standard RB test [14][15][16][17] (Instituto Nacional de Salud, Lima, Peru) and a lateral flow assay (LFA) for detection of specific immunoglobulin M (IgM) and IgG antibodies 18 were used for screening; a positive result on either test was sufficient to be considered positive. Comprehensive clinical and demographic data including personal history of brucellosis and white cheese consumption were recorded.…”

Section: Methodsmentioning

confidence: 99%

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A Foodborne Outbreak of Brucellosis at a Police Station Cafeteria, Lima, Peru

Román¹,

Castillo²,

Gilman³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. Brucella melitensis is highly infectious for humans and can be transmitted to humans in a number of epidemiological contexts. Within the context of an ongoing brucellosis surveillance project, an outbreak at a Peruvian police officer cafeteria was discovered, which led to active surveillance (serology, blood culture) for additional cases among 49 police officers who had also eaten there. The cohort was followed up to 18 months regardless of treatment or symptoms. Active surveillance estimated the attack rate at 26.5% (13 of 49). Blood cultures from four cases were positive; these isolates were indistinguishable using multiple locus variable number tandem repeat analysis. This investigation indicates the importance of case tracking and active surveillance for brucellosis in the context of potential common source exposure. These results provide rationale for public health investigations of brucellosis index cases including the bioterrorism-related dissemination of Brucella.

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Development and evaluation of a rapid immunochromatographic test for mumps‐specific IgM in oral fluid specimens and use as a matrix for preserving viral nucleic acid for RT‐PCR

Warrener¹,

Slibinskas²,

Brown³

et al. 2010

Journal of Medical Virology

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The use of oral fluid specimens for diagnosis of mumps by detection of virus-specific IgM or nucleic acid is now well established. The utility of oral fluids would be improved further if testing could be performed closer to the patient. A near patient test (NPT) for the detection of mumps-specific IgM in oral fluid specimens was developed and evaluated using 196 oral fluid specimens from suspected cases of mumps and measles. Compared to enzyme immunoassay (EIA), the sensitivity, specificity, positive and negative predictive value of the mumps IgM NPT were 79.5%, 100%, 100%, and 72.6%, respectively. On oral fluid specimens with a test to negative control optical density ratio (T/N) > or =10 in the EIA, sensitivity of the NPT increased to 95.2%. To determine whether viral nucleic acid from oral fluid was preserved on the NPT strips used for IgM detection, reverse transcription-polymerase chain reaction (RT-PCR) was performed on 58 oral fluids before and after testing on NPT strips. In RT-PCR-positive oral fluids, mumps virus was detected in 19/21 (90.5%) specimens extracted from NPT strips. Mumps virus RNA was not detected in 37/37 (100%) NPT strips from mumps RT-PCR-negative oral fluid specimens. Mumps IgM NPT is rapid and simple to perform, with an acceptable level of performance for confirmation of a clinical diagnosis outside of the laboratory. The NPT strip is also a suitable matrix for preserving nucleic acid, enabling virus-specific RT-PCR to be performed. J. Med. Virol. 82:485-493, 2010. (c) 2010 Wiley-Liss, Inc.

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ImmunochromatographicBrucella-Specific Immunoglobulin M and G Lateral Flow Assays for Rapid Serodiagnosis of Human Brucellosis

Cited by 100 publications

References 24 publications

Laboratory diagnosis of human brucellosis in Egypt and persistence of the pathogen following treatment

Laboratory diagnosis of human brucellosis in Egypt and persistence of the pathogen following treatment

A Foodborne Outbreak of Brucellosis at a Police Station Cafeteria, Lima, Peru

Development and evaluation of a rapid immunochromatographic test for mumps‐specific IgM in oral fluid specimens and use as a matrix for preserving viral nucleic acid for RT‐PCR

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